Strain number NIES-2237  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Chlamydomonas reinhardtii Dangeard  
Synonym Chlamydomonas morieri Dangeard 1888; Chlamydomonas pseudodebaryana Brabez 1941  
Former name  
Common name Green alga  
Locality (Date of collection) near Amherst Massachusetts USA  
Latitude / Longitude  
Habitat (Isolation source)  
History < IAM (2007) < Mihara, S. (1991)  
Isolator (Date of isolation)  
Identified by  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  TAP (agar)  
Temperature:  20 C
Light intensity:  2-3 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  6 M  
Gene information 18S rRNA ( AB701552 )  
Cell size (min - max) 5 - 18 μm  
Organization Unicellular; Flagellate 
Characteristics Phototaxis ; D-Threonine aldolase (Hirato et al. 2007) ; D-amino acids ; Cell wall less mutant of NIES-2238 (IAM C-541) ; Loss of nitrate reductase ; Unable to use nitrate as nitrogen source (Pröschold et al. 2005)  
Other strain no. Other collection strain no. : IAM C-540
Other strain no. : cw-15 strain  
Remarks Cryopreserved; Axenic 
Movie  
Reference
Tokunaga, S., Morimoto, D., Koyama, T., Kubo, Y., Shiroi, M., Ohara, K., Tokuhiro, H., Mori, Y., Nakagawa, S., Sawayama, S. 2021 Enhanced lutein production in Chlamydomonas reinhardtii by overexpression of the lycopene epsilon cyclase gene. Appl. Biochem. Biotechnol., ,
Keywords: Lutein; Lycopene epsilon cyclase; Carotenoid; Chlamydomonas reinhardtii; Transgene
Strain(s): 2237 
PubMed: 33528746
DOI: 10.1007/s12010-021-03524-w

Hirato, Y., Goto, M., Tokuhisa, M., Tanigawa, M., Nishimura, K. 2017 Crystallization and X-ray analysis of D-threonine aldolase from Chlamydomonas reinhardtii. Acta Crystallogr. F-Struct. Biol., F73, 86-89.
Keywords: D-threonine aldolase; Chlamydomonas reinhardtii; D-amino acids; crystallization
Strain(s): 2237 
PubMed: 28177318
DOI: 10.1107/S2053230X1602063X

Hirato, Y., Tokuhisa, M., Tanigawa, M., Ashida, H., Tanaka, H., Nishimura, K. 2017 Cloning and characterization of d-threonine aldolase from the green alga Chlamydomonas reinhardtii. Phytochemistry, 135, 18-23.
Keywords: Chlamydomonas reinhardtii; Chlamydomonadaceae; Green alga; Enzyme characterization; d-Amino acid; d-Threonine aldolase; Pyridoxal 5’-phosphate
Strain(s): 2237 
PubMed: 28038776
DOI: 10.1016/j.phytochem.2016.12.012

Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12.
Strain(s): 158437438440884968102110221048173318481849220122022203220422062207220822092210221122122213221422152216221822192220222222232224222522282229223022312233223422352236223722382239224022412242224322462247224823142315231623172318231923202321232223232324246224632499257525762577257825793284 

Pröschold, T., Harris, E. H., Coleman, A. W. 2005 Portrait of a species: Chlamydomonas reinhardtii. Genetics, 170, 1601-1610.
Keywords: Chlamydomonas; Internal Transcribed Spacer; phylogenetics; population genetics; ribosomal cistrons
Strain(s): 22372238 
PubMed: 15956662
DOI: 10.1534/genetics.105.044503

Kimpel, D. L., Togasaki, R. K., Miyachi, S. 1983 Carbonic anhydrase in Chlamydomonas reinhardtii I. Localization. Plant Cell Physiol., 24, 255-259.
Keywords: carbonic anhydrase; cell wall-less mutant; Chyamydomonas reinhardtii; periplasm; wall lysing enzyme
Strain(s): 22372238 
DOI: 10.1093/pcp/24.2.255

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