Strain number NIES-2215  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Chloromonas difformis Makino, Matsuzaki & Nozaki  
Synonym  
Former name Formerly identified as Chlamydomonas gerloffii, re-named based on Makino et al. 2019[2019 Oct]  
Common name Green alga  
Locality (Date of collection) Schonhengst, Czechoslovakia  
Latitude / Longitude 49.74617 / 16.574678 
Habitat (Isolation source) Freshwater (Pond water)  
History < IAM (2007) < UTEX (1996);  
Isolator (Date of isolation) Ettl, H. (1962-**-**)  
Identified by  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 C
Light intensity:  3-5 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  6 M  
Gene information 18S rRNA ( AB701536 ) , rbcS ( LC547876 LC547877 LC547878 )  
Cell size (min - max)  
Organization Unicellular; Flagellate 
Characteristics Phototaxis  
Other strain no. Other collection strain no. : IAM C-554 (=C-233); CCAP 11/72; SAG 20.72; UTEX 1348
Other strain no. : Ettl 136  
Remarks Cryopreserved; Axenic 
Movie  
Reference
Matsuzaki, R., Suzuki, S., Yamaguchi, H., Kawachi, M., Kanesaki, Y., Yoshikawa, H., Mori, T., Nozaki, H. 2021 The Rubisco small subunits in the green algal genus Chloromonas provide insights into evolutionary loss of the eukaryotic carbon-concentrating organelle, the pyrenoid BMC Ecol. Evol., 21, 11 (article ID).
Keywords: Chloromonas; Evolution; Green algae; Hydrophobicity of RBCS helices; Pyrenoid; Pyrenoid loss; Rubisco small subunit (RBCS)
Strain(s): 14442442522072212221522422243249937534284 
PubMed: 33514317
DOI: 10.1186/s12862-020-01733-1

Makino, T., Matsuzaki, R., Suzuki, S., Yamaguchi, H., Kawachi, M., Nozaki, H. 2019 Taxonomic re-examination of two NIES strains of“Chlamydomonas” within the Reticulata group of the genus Chloromonas (Volvocales, Chlorophyceae). Microb. Resour. Syst., 35, 13-23.
Keywords: Chlamydomonas gerloffii; Chlamydomonas subangulosa; Chloromonas difformis nom. nov.; Chloromonas typhlos; molecular phylogeny; morphology; taxonomy
Strain(s): 22152243 

Sakurai, K., Mori, N., Sato, N. 2014 Detection and characterization of phosphatidylcholine in various strains of the genus Chlamydomonas (Volvocales, Chlorophyceae). J. Plant Res., 127, 641-650.
Keywords: Chlamydomonas; Delta 5 desaturase; Diacylglyceryl-N,N,N-trimethylhomoserine; Fatty acid; Phosphatidylcholine
Strain(s): 22022207221222152219222522302242231624622499 
PubMed: 24947506
DOI: 10.1007/s10265-014-0644-0

Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12.
Strain(s): 158437438440884968102110221048173318481849220122022203220422062207220822092210221122122213221422152216221822192220222222232224222522282229223022312233223422352236223722382239224022412242224322462247224823142315231623172318231923202321232223232324246224632499257525762577257825793284 

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