Strain number NIES-4336  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Volvox rousseletii G.S.West  
Synonym  
Former name  
Common name Green alga ; Volvocine alga  
Locality (Date of collection) Lake Sagami, Kanagawa, Japan (2015-06-10)  
Latitude / Longitude 35.61041 / 139.18583 
Habitat (Isolation source) Freshwater (Reservoir water)  
History < Nozaki, Hisayoshi  
Isolator (Date of isolation) Nozaki, Hisayoshi (2015-05-10)  
Identified by Kinbara, Ryosuke; Nozaki, Hisayoshi  
State of strain Subculture; Unialgal; Clonal; Axenic[2022 Mar]  
Culture condition
(Preculture condition)
Medium:  AF-6/3AF-6  
Temperature:  20 C
Light intensity:  24-26 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S-ITS1-5.8S-ITS2-28S ( LC493797 ) , rbcL ( LC493808 ) , psbC ( LC493810 )  
Cell size (min - max)  
Organization Colonial; Flagellate 
Characteristics Phototaxis ; Dioecious ; Heterothallic ; Male  
Other strain no. Other strain no. : 2015-0610-3v6  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1"; Axenic 
Movie  
Reference
Yamagishi, S., Yamamoto, K., Takahashi, K., Kawai-Toyooka, H., Suzuki, S., Matsuzaki, R., Yamaguchi, H., Kawachi, M., Higashiyama, T., Nozaki, H. 2023 Evolutionary analysis of MID homologs during the transition from homothallic species to heterothallic species in Volvox sect. Volvox (Chlorophyceae). Phycol. Res., 72, 46-55.
Keywords: male gamete; mating system; minus dominance gene (MID); molecular evolution; sex; sperm packet; volvocine green algae
Strain(s): 28945646054154454556957257458265265365473285185285787289189213621363170817101859245725692735273627403382338337803782378638743984398639884021402543364432453545364537 
DOI: 10.1111/pre.12538

Nozaki, H., Mahakham, W., Heman, W., Matsuzaki, R., Kawachi, M. 2020 A new preferentially outcrossing monoicous species of Volvox sect. Volvox (Chlorophyta) from Thailand. PLoS One, 15, e0235622 (article ID).
Strain(s): 273627403382338338744021433644324433443444354436 
PubMed: 32614898
DOI: 10.1371/journal.pone.0235622

Kimbara, R., Isaka, N., Matsuzaki, R., Kawai-Toyooka, H., Kawachi, M., Nozaki, H. 2019 Morphological and molecular identification of the dioecious “African species Volvox rousseletii (Chlorophyceae) in the water column of a Japanese lake based on field-collected and cultured materials. PLoS One, 14, e0221632 (article ID).
Strain(s): 4336433743384339434043414342 
PubMed: 31465476
DOI: 10.1371/journal.pone.0221632

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