Strain number NIES-4320  
Phylum Streptophyta  
Class Zygnematophyceae  
Scientific name Closterium peracerosum-strigosum-littorale complex  
Synonym  
Former name  
Common name Desmid  
Locality (Date of collection) Asahata retarding basin, Ushida, Aoi-ku, Shizuoka, Shizuoka, Japan (2013-07-20)  
Latitude / Longitude 35.015750 / 138.402806 
Habitat (Isolation source) Freshwater (Pond water)  
History < Tsuchikane, Yuki  
Isolator (Date of isolation) Tsuchikane, Yuki (2013-07-20)  
Identified by Tsuchikane, Yuki  
State of strain Subculture; Unialgal; Clonal; Axenic[2022 Mar]  
Culture condition
(Preculture condition)
Medium:  CA  
Temperature:  20 C
Light intensity:  17 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S rRNA ( LC361440 )  
Cell size (min - max) 139 - 143 μm  
Organization Unicellular 
Characteristics Heterothallic ; Isogamou ; Mating type (+) ; Mating group G (Tsuchikane et al. 2018)  
Other strain no. Other strain no. : Asa13-21  
Remarks Axenic 
Movie  
Reference
Kawaguchi, Y. W., Tsuchikane, Y., Tanaka, K., Taji, T., Suzuki, Y., Toyoda, A., Ito, M., Watano, Y., Nishiyama, T., Sekimoto, H., Tsuchimatsu, T. 2023 Extensive copy number variation explains genome size variation in the unicellular Zygnematophycean alga, Closterium peracerosum–strigosum–littorale complex. Genome Biol. Evol., 15, evad115 (article ID).
Keywords: gene duplication; dosage compensation; copy number variation; green algae
Strain(s): 53545859626364656826126664319432043214548455045514552 
PubMed: 37348049
DOI: 10.1093/gbe/evad115

Tsuchikane, Y., Kobayashi, H., Kato, M., Watanabe, J., Wu, J. T., Sekimoto, H. 2018 Identification of a new mating group and reproductive isolation in the Closterium peracerosum–strigosum–littorale complex. J. Plant Res., 131, 735–746.
Keywords: Alga; Closterium; Mating group; Reproductive isolation; Sex pheromone; Speciation
Strain(s): 535455646566676826126243194320 
PubMed: 29948484
DOI: 10.1007/s10265-018-1043-8

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