Strain number | NIES-2237 | |||
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Phylum | Chlorophyta | |||
Class | Chlorophyceae | |||
Scientific name | Chlamydomonas reinhardtii P.A.Dangeard | |||
Synonym | Chlamydomonas morieri Dangeard 1888; Chlamydomonas pseudodebaryana Brabez 1941 | |||
Former name | ||||
Common name | Green alga | |||
Locality (Date of collection) | near Amherst, Massachusetts, USA (locality of wild type strain (NIES-2238)) | |||
Latitude / Longitude | ||||
Habitat (Isolation source) | ||||
History | < IAM (2007) < Mihara, S. (1991) | |||
Isolator (Date of isolation) | ||||
Identified by | ||||
State of strain | Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb] | |||
Culture condition (Preculture condition) |
Medium:
TAP (agar)
Temperature: 20 C Light intensity: 2-3 µmol photons/m2/sec, L/D cycle: 10L:14D Duration: 6 M |
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Gene information | 18S rRNA ( AB701552 ) | |||
Cell size (min - max) | 5 - 18 μm | |||
Organization | Unicellular; Flagellate | |||
Characteristics | Phototaxis ; D-Threonine aldolase (Hirato et al. 2007) ; D-amino acids ; Cell wall less mutant of NIES-2238 ; Loss of nitrate reductase ; Unable to use nitrate as nitrogen source (Pröschold et al. 2005) | |||
Other strain no. |
Other collection strain no. : IAM C-540
Other strain no. : cw-15 strain |
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Remarks | Cryopreserved; Axenic | |||
Movie |
Reference |
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Tokunaga, S., Morimoto, D., Koyama, T., Kubo, Y., Shiroi, M., Ohara, K., Tokuhiro, H., Mori, Y., Nakagawa, S., Sawayama, S.
2021
Enhanced lutein production in Chlamydomonas reinhardtii by overexpression of the lycopene epsilon cyclase gene.
Appl. Biochem. Biotechnol.,
193,
1967–1978.
Keywords: Lutein; Lycopene epsilon cyclase; Carotenoid; Chlamydomonas reinhardtii; Transgene Strain(s): 2237 PubMed: 33528746 DOI: 10.1007/s12010-021-03524-w Hirato, Y., Goto, M., Tokuhisa, M., Tanigawa, M., Nishimura, K. 2017 Crystallization and X-ray analysis of D-threonine aldolase from Chlamydomonas reinhardtii. Acta Crystallogr. F-Struct. Biol., F73, 86-89. Keywords: D-threonine aldolase; Chlamydomonas reinhardtii; D-amino acids; crystallization Strain(s): 2237 PubMed: 28177318 DOI: 10.1107/S2053230X1602063X Hirato, Y., Tokuhisa, M., Tanigawa, M., Ashida, H., Tanaka, H., Nishimura, K. 2017 Cloning and characterization of d-threonine aldolase from the green alga Chlamydomonas reinhardtii. Phytochemistry, 135, 18-23. Keywords: Chlamydomonas reinhardtii; Chlamydomonadaceae; Green alga; Enzyme characterization; d-Amino acid; d-Threonine aldolase; Pyridoxal 5’-phosphate Strain(s): 2237 PubMed: 28038776 DOI: 10.1016/j.phytochem.2016.12.012 Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12. Strain(s): 158, 437, 438, 440, 884, 968, 1021, 1022, 1048, 1733, 1848, 1849, 2201, 2202, 2203, 2204, 2206, 2207, 2208, 2209, 2210, 2211, 2212, 2213, 2214, 2215, 2216, 2218, 2219, 2220, 2222, 2223, 2224, 2225, 2228, 2229, 2230, 2231, 2233, 2234, 2235, 2236, 2237, 2238, 2239, 2240, 2241, 2242, 2243, 2246, 2247, 2248, 2314, 2315, 2316, 2317, 2318, 2319, 2320, 2321, 2322, 2323, 2324, 2462, 2463, 2499, 2575, 2576, 2577, 2578, 2579, 3284 Pröschold, T., Harris, E. H., Coleman, A. W. 2005 Portrait of a species: Chlamydomonas reinhardtii. Genetics, 170, 1601-1610. Keywords: Chlamydomonas; Internal Transcribed Spacer; phylogenetics; population genetics; ribosomal cistrons Strain(s): 2237, 2238 PubMed: 15956662 DOI: 10.1534/genetics.105.044503 Kimpel, D. L., Togasaki, R. K., Miyachi, S. 1983 Carbonic anhydrase in Chlamydomonas reinhardtii I. Localization. Plant Cell Physiol., 24, 255-259. Keywords: carbonic anhydrase; cell wall-less mutant; Chyamydomonas reinhardtii; periplasm; wall lysing enzyme Strain(s): 2237, 2238 DOI: 10.1093/pcp/24.2.255 |
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