Strain number NIES-2242  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Chlamydomonas sphaeroides Gerloff  
Synonym  
Former name Chlamydomonas iyengari Mitra  
Common name Green alga  
Locality (Date of collection) India  
Latitude / Longitude  
Habitat (Isolation source)  
History < IAM (2007) < BIU (UTEX; 1967);  
Isolator (Date of isolation) Pringsheim, E. G.  
Identified by  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 C
Light intensity:  2-3 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  6 M  
Gene information Whole-genome ( BDDC00000000 ) , 18S rRNA ( AB701556 ) , EPT ( LC068986 ) , ECT ( LC068985 ) , EKI ( LC068805 )  
Cell size (min - max) 8 - 12 μm  
Organization Unicellular; Flagellate 
Characteristics Phototaxis ; Genome decoded strain (Hirashima et al. 2016)  
Other strain no. Other collection strain no. : IAM C-255; CCAP 11/14; UTEX 221; SAG 25.72; CGC CC-1811
 
Remarks Cryopreserved; Axenic 
Movie  
Reference
Flores, D. C., Fricke, M., Wesp, V., Desirò, D., Kniewasser, A., Hölzer, M., Marz, M., Mittag, M. 2021 A marine Chlamydomonas sp. emerging as an algal model. J. Phycol., 57, 54-69.
Strain(s): 8822202220422112218222022232236224122422248 
PubMed: 33043442
DOI: 10.1111/jpy.13083

Hirashima, T., Toyoshima, M., Moriyama, T., Sato, N. 2018 Evolution of the phosphatidylcholine biosynthesis pathways in green algae: Combinatorial diversity of methyltransferases. J. Mol. Evol., 86, 68-76.
Keywords: Chlamydomonas; Metabolic diversity; Phosphatidylcholine biosynthesis; Phosphoethanolamine-N-methyltransferase; Phospholipid-N-methyltransferase
Strain(s): 2242 
PubMed: 29330556
DOI: 10.1007/s00239-017-9826-4

Hirashima, T., Toyoshima, M., Moriyama, T., Nakamura, Y., Sato, N. 2017 Characterization of phosphoethanolamine-N-methyltransferases in green algae. Biochem. Biophys. Res. Commun., 488, 141-146.
Keywords: Green algal evolution; Chlamydomonas; Phosphatidylcholine biosynthesis; Phosphoethanolamine-N-methyltransferase
Strain(s): 2242 
PubMed: 2848352
DOI: 10.1016/j.bbrc.2017.05.026

Hirashima, T., Tajima, N., Sato, N. 2016 Draft genome sequences of four species of Chlamydomonas containing phosphatidylcholine. GenomeA, 4, e01070-16 (article ID).
Strain(s): 2242 
PubMed: 27688324
DOI: 10.1128/genomeA.01070-16

Sato, N., Mori, N., Hirashima, T., Moriyama, T. 2016 Diverse pathways of phosphatidylcholine biosynthesis in algae as estimated by labeling studies and genomic sequence analysis. Plant J., 87, 281–292.
Keywords: Chlamydomonas; Cyanidioschyzon merolae; phosphatidylcholine biosynthesis; phosphatidylethanolamine methyltransferase; phosphoethanolamine methyltransferase
Strain(s): 2242 
PubMed: 27133435
DOI: 10.1111/tpj.13199

Nakada, T., Ito, T., Tomita, M. 2016 18S ribosomal RNA gene phylogeny of a colonial volvocalean lineage (Tetrabanaceae-Goniaceae-Volvocaceae, Volvocales, Chlorophyceae) and its close relatives. J. Jpn. Bot., 91, 345-354.
Keywords: 18S rRNA; colonial; green algae; molecular phylogeny; unicellular; Volvocales
Strain(s): 4568832242 

Sakurai, K., Mori, N., Sato, N. 2014 Detection and characterization of phosphatidylcholine in various strains of the genus Chlamydomonas (Volvocales, Chlorophyceae). J. Plant Res., 127, 641-650.
Keywords: Chlamydomonas; Delta 5 desaturase; Diacylglyceryl-N,N,N-trimethylhomoserine; Fatty acid; Phosphatidylcholine
Strain(s): 224223162499 
PubMed: 24947506
DOI: 10.1007/s10265-014-0644-0

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