Strain number NIES-81  
Phylum Cyanophyta  
Class Cyanophyceae  
Scientific name Aphanizomenon flos-aquae Ralfs ex Bornet & Flahault f. gracile (Lemmermann) Elenkin  
Synonym Byssus flos-aquae Linnaeus 1753; Conferva flos-aquae (Linnaeus) Roth 1806; Oscillatoria flos-aquae (Linnaeus) C.Agardh 1812; Nostoc flos-aquae (Linnaeus) Lyngbye 1819; Nostoc papyraceum S.F.Gray 1821; Trichodesmium flos-aquae Ehrenberg 1830; Sphaerozyga flos-aquae (Linnaeus) Corda 1836; Limnochlide flos-aquae (Linnaeus) Kützing 1843; Micraloa flos-aquae (Linnaeus) Trevisan 1845; Trichormus flos-aquae (Linnaeus) Ralfs 1850; Aphanizomenon cyaneum Ralfs ex Bornet & Flahault 1888; Aphanizomenon holsaticum P.G.Richter 1891; Aphanizomenon americanum E.G.Reinhard 1896; Aphanizomenon flos-aquae f. holsaticum (P.G.Richter) Elenkin 1938; Aphanizomenon flos-aquae f. typicum Elenkin 1938  
Former name  
Common name Blue-green alga ; Cyanobacteria  
Locality (Date of collection) Lake Kasumigaura, Ibaraki, Japan (1978-01-24)  
Latitude / Longitude 36.0428477398 / 140.373233439 
Habitat (Isolation source) Freshwater (Lake water)  
History < TAC;  
Isolator (Date of isolation) Watanabe, Masayuki (1978-02-15)  
Identified by Watanabe, Masayuki  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  CB  
Temperature:  25 C
Light intensity:  20-25 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  20 D  
Gene information Whole-genome ( AZYY00000000 ) , 16S rRNA ( AF317632 AJ293131 AY038035 ) , rbcL, rbcX ( AJ293170 ) , ITS1 ( AJ293213 )  
Cell size (min - max) - 5 μm  
Organization Filamentous 
Characteristics Cyanobacterial water bloom (aoko) ; anatoxin (-) ; saxitoxin (-) ; Offensive odor ; Draft genome sequence (Cao et al. 2014)  
Other strain no. Other strain no. : TAC 1 (K-TAN-1)  
Remarks Cryopreserved; Axenic 
Movie  
Reference
Carrell, A. A., Lawrence, T. J., Cabugao, K. G. M., Carper, D. L., Pelletier, D. A., Lee, J. H., Jawdy, S., Grimwood, J., Schmutz, J., Hanson, P. J., Shaw, A. J., Weston, D. J. 2022 Habitat-adapted microbial communities mediate Sphagnum peatmoss resilience to warming. New Phytol., 234, 2111-2125.
Keywords: climate change; heat tolerance; microbiome transfer; moss; peatland; Sphagnum; symbiosis; synthetic communities
Strain(s): 2581209821002107 
DOI: 10.1111/nph.18072

Dreher, T. W., Davis, E. W., Mueller, R. S., Otten, T. G. 2021 Comparative genomics of the ADA clade within the Nostocales. Harmful Algae, 104, 102037 (article ID).
Keywords: Dolichospermum; Aphanizomenon; Anabaena; Mobilome; Genome synteny; Cyanotoxin genes
Strain(s): 8081806 
PubMed: 34023075
DOI: 10.1016/j.hal.2021.102037

Monteiro, P. R., do Amaral, S. C., Siqueira, A. S., Xavier, L. P., Santos, A. V. 2021 Anabaenopeptins: What We Know So Far. Toxins, 13, 522 (article ID).
Keywords: cyanobacteria; peptide; NRPS; anabaenopeptin
Strain(s): 81204595 
PubMed: 34437393
DOI: 10.3390/toxins13080522

Asukabe, H., Akahori, S., Ueno, E., Nakayama, T., Yamashita, R., Arii, S., Harada, K., Imanishi, S. Y. 2020 Cyanobacterial classification with the toxicity using MALDI Biotyper. J. Am. Soc. Mass Spectrom., 31, 1572-1578.
Keywords: cyanobacteria; MALDI Biotyper; microcystins; profiling
Strain(s): 27394273818789100101102103104105107111112207208230266298416433478510512515592593604611808809835843846847905928931932945981989102510431058106110641068106910711076108510991101110511131115112611331134113511441157121516421643165216681672167316771678169820872131241226653708373337343736375537563757 
PubMed: 32501712
DOI: 10.1021/jasms.0c00148

Pham, T.-L., Bui, M. H., Driscoll, M., Shimizu, K., Motoo, U. 2020 First report of geosmin and 2-methylisoborneol (2-MIB) in Dolichospermum and Oscillatoria from Vietnam. Limnology, 22, 43–56.
Keywords: Off-flavour compounds; Taste and odour; Tropical water; Cyanobacteria
Strain(s): 19338181416481879 
DOI: 10.1007/s10201-020-00630-2

Cao, H., Shimura, Y., Steffen, M. M., Yang, Z., Lu, J., Joel, A., Jenkins, L., Kawachi, M., Yin, Y., Garcia-Pichel, F. 2020 The trait repertoire enabling cyanobacteria to bloom assessed through comparative genomic complexity and metatranscriptomics. mBio, 11, e01155-20 (article ID).
Keywords: Symbiodiniaceae; Durusdinium trenchii; WGS; MAAs; GMC oxidoreductase
Strain(s): 81843 
PubMed: 32605986
DOI: 10.1128/mBio.01155-20

Lin, W., Zhao, D., Luo, J. 2018 Distribution of alkaline phosphatase genes in cyanobacteria and the role of alkaline phosphatase on the acquisition of phosphorus from dissolved organic phosphorus for cyanobacterial growth. J. Appl. Phycol., 30, 839-850.
Keywords: Alkaline; phosphatase; Cyanobacteria; Dissolved organic phosphorus; Distribution
Strain(s): 303981213025493756 
DOI: 10.1007/s10811-017-1267-3

Driscoll, C. B., Meyer, K. A., Šulčius, S., Brown, N. M., Dick, G. J., Cao, H., Gasiūnas, G., Timinskas, A., Yin, Y., Landry, Z. C., Otten, T. G., Davis, T. W., Watson, S. B., Dreher, T. W. 2018 A closely-related clade of globally distributed bloom-forming cyanobacteria within the Nostocales. Harmful Algae, 77, 93-107.
Keywords: Genomics; Metagenomics; Filamentous cyanobacteria
Strain(s): 81 
PubMed: 30005805
DOI: 10.1016/j.hal.2018.05.009

Kerfeld, CA., Melnicki, MR., Sutter M., Dominguez-Martin, MA. 2017 Structure, function and evolution of the cyanobacterial orange carotenoid protein and its homologs. New Phytol., 215, 937-951.
Keywords: carotenoids; carotenoproteins; cyanobacteria; nonphotochemical quenching; photoprotection; phylogenomics; protein structure; singlet oxygen (1O2) quenching
Strain(s): 3981843 
PubMed: 28675536
DOI: 10.1111/nph.14670

Chiu, Y.-T., Yen, H.-K., Lin, T.-F. 2016 An alternativemethodtoquantify2-MIB producing cyanobacteriain in drinking water reservoirs: Method development and field applications. Environ. Res., 151, 618-627.
Keywords: Cyanobacteria; 2-methylisoborneol(2-MIB); 2-MIB-synthesis gene(mibC gene); Tasteandodor; Real-timePCR
Strain(s): 8151282490521042116 
PubMed: 27607443
DOI: 10.1016/j.envres.2016.08.034

Cao, H., Shimura, Y., Kawachi, M., Yin, Y. 2014 Draft genome sequence of the toxic bloom-forming cyanobacterium Aphanizomenon flos-aquae NIES-81. GenomeA, 2, e00044-14 (article ID).
Strain(s): 81 
PubMed: 24526634
DOI: 10.1128/genomeA.00044-14

Wacklin, P., Hoffmann, L., Komárek, J. 2009 Nomenclatural validation of the genetically revised cyanobacterial genus Dolichospermum (Ralfs ex Bornet et Flahault) comb. nova. Fottea, 9, 59-64.
Keywords: Cyanobacteria; Anabaena; Dolichospermum; taxonomy; nomenclatoric validation
Strain(s): 81 

Koskenniemi, K., Lyra, C., Rajaniemi-Wacklin, P., Jokela, J., Sivonen, K. 2007 Quantitative real-time PCR detection of toxic Nodularia cyanobacteria in the Baltic Sea. Appl. Environ. Microbiol., 73, 2173-2179.
Strain(s): 8189102 
PubMed: 17277219
DOI: 10.1128/AEM.02746-06

Hosoya, R., Hamana, K., Isobe, M., Yokota, A. 2005 Polyamine distribution profiles within Cyanobacteria. Microbiol. Cult. Coll., 21, 3-8.
Keywords: cyanobacteria; homospermidine; polyamine; spermidine
Strain(s): 19212228303437414445507378798081204208510512515592593610843846847209320962098210021022107210821182119212321242125212621282129213121323259327532763277 

Hirabayashi, R. & Imamura, N. 2003 Action mechanism of a selective anti-cyanobacterial compound, argimicin A. J. Antibiotics, 56, 154-159.
Strain(s): 414581102204230298592 
PubMed: 12715875
DOI: 10.7164/antibiotics.56.154

Yamaguchi, T., Kobayashi, Y., Adachi, K., Imamura, N. 2003 Argimicins B and C, new anti-cyanobacterial compounds produced by Sphingomonas sp. M-17. J. Antibiot., 56, 655-657.
Strain(s): 4581102230298592 
PubMed: 14513910
DOI: 10.7164/antibiotics.56.655

Jung, J.-M., Lee, Y.-J., Park, H.-K., Jung, E.-Y., Joo, G.-J. 2003 The analysis of cyanobacterial neurotoxins by high-performance liquid chromatography-mass spectrometry. Algae, 18, 233-238.
Keywords: anatoxin-a; Aphanizomenon flos-aquae; cyanobacterial toxins; LC-MS; saxitoxin
Strain(s): 81 
DOI: 10.4490/ALGAE.2003.18.3.23

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 192122232425262728293031323334353637383940414445465051525354555658596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123125127128129130131132133134135137138139144147151152153154155156157158159160162163164165166167168170171172173174175176177178179180181182183185186187188189191192193194197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268287288289290294295297298299300301302303305306308309310312313329334337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Yuki, Y., Yoshida, T., Hiroishi, S. 2002 Molecular detection of the toxic cyanobacteria [Yûdoku aoko no bunshi-shikibetsu to yosatsu he-no ôyô]. In Series of Fisheries Science Vol. 143, Prevention and Extermination Strategies of Harmful Algal Blooms [Suisan-gaku shirîzu 134, Yûgai-yûdoku sôrui burûmu no yobô to kujo] , Eds. by Hiroishi, S., Imai, I. & Ishimaru, T., Koseisha-Koseikaku, Tokyo, pp. 43-53 (in Japanese).
Strain(s): 738187888990919899100101102104105106108109110111112204230298299604 

Gugger, M., Lyra, C., Henriksen, P., Coute, A., Humbert, J.-F., Sivonen, K. 2002 Phylogenetic comparison of the cyanobacterial genera Anabena and Aphanizomenon. Int. J. Syst. Evol. Microbiol., 52, 1867-1880.
Keywords: Anabaena; Aphanizomenon; 16S rDNA; 16S-23S rDNA; RubisCO spacer
Strain(s): 81 
PubMed: 12361299
DOI: 10.1099/00207713-52-5-1867

Lyra, C., Suomalainen, S., Gugger, M., Vezie, C., Sundman, P., Paulin, L., Sivonen, K. 2001 Molecular characterization of planktic cyanobacteria of Anabaena, Aphanizomenon, Microcystis and Planktothrix genera. Int. J. Syst. Evol. Microbiol., 51, 13-526.
Keywords: cyanobacteria; 16S rRNA gene; RFLP; sequencing; REP; ERIC
Strain(s): 41447379818998102104 
DOI: 10.1099/00207713-51-2-513

Murakami, M., Suzuki, S., Itou, Y., Kodani, S., Ishida, K. 2000 New anabaenopeptins, potent carboxypeptidase-A inhibitors from the cyanobacterium Aphanizomenon flos-aquae. J. Nat. Prod., 63, 1280-1282.
Strain(s): 81 
PubMed: 11000037
DOI: 10.1021/np000120k

Tillett, D., Neilan, B. A. 2000 Xanthogenate nucleic acid isolation from cultured and environmental cyanobacteria. J. Phycol., 36, 251-258.
Keywords: cyanobacteria; DNA extraction; nucleic acids; PCR; xanthogenate
Strain(s): 81102107 
DOI: 10.1046/j.1529-8817.2000.99079.x

Neilan, B. A., Dittmann, E., Rouhiainen, L., Bass, R. A., Schaub, V., Sivonen, K., Börner, T. 1999 Nonribosomal peptide synthesis and toxigenicity of Cyanobacteria. J. Bacteriol., 181, 4089-4097.
Strain(s): 19248199102107204 
PubMed: 10383979
DOI: 10.1128/JB.181.13.4089-4097.1999

Li, R., Yokota, A., Sugiyama, J., Watanabe, M., Hiroki, M., Watanabe, M. M. 1998 Chemotaxonomy of planktonic cyanobacteria based on non-polar and 3-hydroxy fatty acid composition. Phycol. Res., 46, 21-28.
Keywords: 3-hydroxy fatty acids; chemotaxonomy; fatty acid; non-polar fatty acids; planktonic cyanobacteria
Strain(s): 2740447678798081878998102104111112204205207298604810821825826 
DOI: 10.1111/j.1440-1835.1998.tb00093.x

Neilan, B. A., Jacobs, D., Goodman, A. E. 1995 Genetic diversity and phylogeny of toxic cyanobacteria determined by DNA polymorphisms within the phycocyanin locus. Appl. Environ. Microbiol., 61, 3875-3883.
Strain(s): 244041447376788081878998102107111112204207298 
PubMed: 8526499

Soma, Y., Imaizumi, T., Yagi, K., Kasuga, S. 1993 Estimation of algal succession in lake water using HPLC analysis of pigments. Can. J. Fish. Aquat. Sci., 50, 1142-1146.
Strain(s): 44738188104111233234333351 

Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title).
Strain(s): 1819212426293139404144454650737475767780818788899091939598991001011201221311351371441512042072132572633794152219223522392579 

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