Strain number NIES-853  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Astrephomene gubernaculifera Pocock  
Synonym  
Former name  
Common name Green alga  
Locality (Date of collection) Emmet County, Michigan, USA (1961-07-**)  
Latitude / Longitude  
Habitat (Isolation source) (Soil)  
History < Nozaki, Hisayoshi < UTEX  
Isolator (Date of isolation) Brooks, A. E. (1962-06-**)  
Identified by Brooks, A. E.  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2017 Dec]  
Culture condition
(Preculture condition)
Medium:  CB-V  
Temperature:  20 C
Light intensity:  7-10 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  20 D  
Gene information ITS1-5.8S-ITS2 ( U66930 )  
Cell size (min - max)  
Organization Colonial; Flagellate 
Characteristics Mixotrophic ; Phototaxis ; Heterothallic  
Other strain no. Other collection strain no. : UTEX 1392
 
Remarks Cryopreserved; Axenic 
Movie  
Reference
Nozaki, H., Mori, F., Tanaka, Y., Matsuzaki, R., Yamashita, S., Yamaguchi, H., Kawachi, K. 2023 Cryopreservation of two species of the multicellular volvocine green algal genus Astrephomene. BMC Microbiol., 23, 16 (article ID).
Keywords: Asexual cycle; Astrephomene; Cryopreservation; Cryoprotectant; Culture collection; Volvocine green algae
Strain(s): 4184195645656288538548554017 
PubMed: 36650459
DOI: 10.1186/s12866-023-02767-3

Brooks, A. E. 1966 The sexual cycle and intercrossing in the genus Astrephomene. J. Protozool., 13, 367-375.
Strain(s): 853854855 
DOI: 10.1111/j.1550-7408.1966.tb01922.x

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