A big image opens when the thumbnail is clicked.(nies-0296.jpg)
Strain number NIES-296  
Phylum Streptophyta  
Class Mesostigmatophyceae  
Scientific name Mesostigma viride Lauterborn  
Former name  
Common name  
Locality (Date of collection) Yoshino Park, Mitsukaido, Ibaraki, Japan (1985-07-03)  
Latitude / Longitude 36.076428 / 140.001716 
Habitat (Isolation source) Freshwater (Lake water)  
History < Suda, Shoichiro  
Isolator (Date of isolation) Suda, Shoichiro (1985-07-08)  
Identified by Inouye, Isao  
State of strain Subculture; Unialgal; Clonal; Axenic[2018 Jan]  
Culture condition
(Preculture condition)
Medium:  C  
Temperature:  20 C
Light intensity:  24-39 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  25 D  
Gene information Whole-genome ( RPFO01000000 ) , Mitochondria DNA ( AF353999 ) , Plastid DNA ( AF166114 ) , Root cap protein 1-8 mRNAs ( DQ287936-DQ287943 ) , ftsZ ( AB673433 )  
Cell size (min - max) - 18 μm  
Organization Unicellular; Flagellate 
Characteristics Genome decoded strain (Lemieux, C. et al. 2000, Turmel, M. et al. 2002b, Liang et al. 2020)  
Other strain no. Other strain no. : KY-14  
Remarks Unstable; Fragile species to transportation stresses; Read and agree "How to order 4.1"; Axenic 
Movie https://www.youtube.com/watch?v=En3oZr0DE64&list=PLFTJlLsZcVbiJY3ygYAPKeVOfDdOh9UEW&index=11  
Aso, M., Matsumae, R., Tanaka, A., Tanaka, R., Takabayashi, A. 2021 Unique peripheral antennas in the photosystems of the streptophyte alga Mesostigma viride. Plant Cell Physiol., ,
Keywords: Green algae; Iso-seq; Mesostigma viride; Native-PAGE; Photosystem
Strain(s): 296 
PubMed: 33416834
DOI: 10.1093/pcp/pcaa172

Bock, N. A., Charvet, S., Burns, J., Gyaltshen, Y., Rozenberg, A., Duhamel ,S., Kim, E. 2021 Experimental identification and in silico prediction of bacterivory in green algae. ISME J., ,
Strain(s): 254296626995 
PubMed: 33649548
DOI: 10.1038/s41396-021-00899-w

Liang, Z., Geng, Y., Ji, C., Du, H., Wong, C. E., Zhang, Q., Zhang, Y., Zhang, P., Riaz, A., Chachar, S., Ding, Y., Wen, J., Wu, Y., Wang, M., Zheng, H., Wu, Y., Demko, V., Shen, L., Han, X., Zhang, P., Gu, X., Yu, H. 2020 Mesostigma viride genome and transcriptome provide insights into the origin and evolution of Streptophyta. Adv. Sci., 7, 1901850 (article ID).
Keywords: Mesostigma viride; evolution; green algae; multicellularity; Streptophyta
Strain(s): 296 
PubMed: 31921561
DOI: 10.1002/advs.201901850

Yokono, M., Takabayashi, A., Kishimoto, J., Fujita, T., Iwai, M., Murakami, A., Akimoto, S., Tanaka, A. 2019 The PSI-PSII megacomplex in green plants. Plant Cell Physiol., 60, 1098–1108.
Keywords: Energy transfer; Nonphotochemical quenching; Photosystem
Strain(s): 14429630322852541 
PubMed: 30753722
DOI: 10.1093/pcp/pcz026

Yang, A., Narechania, A., Kim, E. 2016 Rickettsial endosymbiont in the “early‐diverging” streptophyte green alga Mesostigma viride. J. Phycol., 52, 219-229.
Keywords: endosymbiosis; green algae; Mesostigma ; Rickettsiales; symbiosis; transcriptome
Strain(s): 296475995 
PubMed: 27037587
DOI: 10.1111/jpy.12385

Hamana, K., Niitsu, M., Hayashi, H. 2013 Occurrence of homospermidine and thermospermine as a cellular polyamine in unicellular chlorophyte and multicellular charophyte green algae J. Gen. Appl. Micobiol., 59, 313-319.
Keywords: green alga; homospermidine; polyamine; thermospermine
Strain(s): 1815116025529630636036364072283614091410141114191837216421993374 
PubMed: 24005181
DOI: 10.2323/jgam.59.313

Hua, JM., Smith, D. R., Borza, T., Lee, R. W. 2012 Similar relative mutation rates in the three genetic compartments of Mesostigma and Chlamydomonas. Protist, 163, 105-115.
Keywords: Chlamydomonas; genome architecture; Mesostigma; mutation rate; silent substitution rate
Strain(s): 296476 
PubMed: 21621456
DOI: 10.1016/j.protis.2011.04.003

Miyagishima, S., Suzuki, K., Okazaki, K., Kabeya, Y. 2012 Expression of the nucleus-encoded chloroplast division genes and proteins regulated by the algal cell cycle. Mol. Biol. Evol., 29, 2957-2970.
Keywords: cell cycle; chloroplast division; endosymbiosis; endosymbiotic gene transfer
Strain(s): 296484547133221703377 
PubMed: 22490821
DOI: 10.1093/molbev/mss102

Hua, J., Lee, R.W. 2012 Factors affecting codon bias in the mitochondrial genomes of the Streptophyte Mesostigma viride and the Chlorophyte Chlamydomonas reinhardtii. J. Eucaryot. Microbiol., 59, 287-289.
Keywords: Green algae; mutation; selection; synonymous codon usage
Strain(s): 296 
PubMed: 22340021
DOI: 10.1111/j.1550-7408.2011.00613.x

Nedelcu, A. M., Borza, T., Lee, R. W. 2006 A land-plant specific multigene family in the unicellular Mesostigma argues for its close relationship to Streptophyta. Mol. Biol. Evol., 23, 1011-1015.
Keywords: Mesostigma viride; land plant evolution; Streptophyta; histogenetic meristem; gene family
Strain(s): 296 
PubMed: 16476689
DOI: 10.1093/molbev/msj108

Hamana, K., Aizaki T., Arai, E., Saito, A., Uchikata, K., Ohnishi, H. 2004 Distribution of norspermidine s a cellular polyamine within micro green algae including non-photosynthetic achlorophyllous Polytoma, Polytomella, Prototheca and Helicosporidium. J. Gen. Appl. Microbiol., 50, 289-295.
Keywords: Chlorophyta; green alga; Helicosporidium; norspermidine; polyamine; Polytoma; Polytomella; Prototheca
Strain(s): 18251254255296329483486536 

Yoshii, Y., Takaichi, S., Maoka, T., Inouye, I. 2003 Photosynthetic pigment composition in the primitive green alga Mesostigma viride (Prasinophyceae): phylogenetic and evolutionary implications. J. Phycol., 39, 570-576.
Keywords: abscisic acid; carotenoid; Mesostigma; neoxanthin; siphonaxanthin; siphonaxanthin ester
Strain(s): 296475476477 
DOI: 10.1046/j.1529-8817.2003.02098.x

Laflamme, M., Lee, R. W. 2003 Mitochondrial genome conformation among CW-group Chlorophycean algae. J. Phycol., 39, 213-220.
Keywords: Chlamydomonas; Chlorophyceae; gel electrophoresis; mitochondrial DNA conformation; phylogeny
Strain(s): 296484 
DOI: 10.1046/j.1529-8817.2003.02045.x

Turmel, M., Otis, C., Lemieux, C. 2002b The complete mitochondrial DNA sequence of Mesostigma viride identifies this green alga as the earliest green plant divergence and predicts a highly compact mitochondrial genome in the ancestor of all green plants. Mol. Biol. Evol., 19, 24-38.
Keywords: green aglae; Mesostigma viride;mitochondrial DNA; group I introns; group II introns; trans-splicing
Strain(s): 296 
PubMed: 11752187
DOI: 10.1093/oxfordjournals.molbev.a003979

Turmel, M., Ehara, M., Otis, C., Lemieux, C. 2002a Phylogenetic relationships among streptophytes as inferred from chloroplast small and large subunit rRNA gene sequences. J. Phycol., 38, 364-375.
Keywords: Charophyceae; chloroplast rRNA genes; introns; land plants; phylogeny; Streptophyta
Strain(s): 296484 
DOI: 10.1046/j.1529-8817.2002.01163.x

Schwender, J., Gemünden, C., Lichtenthaler, H. K. 2001 Chlorophyta exclusively use the 1-deoxyxylulose 5-phosphate/2-C-methylerythritol 4-phosphate pathway for the biosynthesis of isoprenoids. Planta, 212, 416-423.
Keywords: Charophyta; 1-Deoxy-D-xylulose; Green alga; Isopentenyl diphosphate; Isoprenoid biosynthesis; Mevalonate
Strain(s): 296 
PubMed: 11289606
DOI: 10.1007/s004250000409

Iwamoto, K., Ikawa, T. 2000 A novel glycolate oxidase requiring flavin mononucleotide as the cofactor in the prasinophycean alga Mesostigma viride. Plant Cell Physiol., 41, 988-991.
Keywords: flavin mononucleotide (FMN)-requiring enzyme; glycolate metabolism; glycolate oxidase; glycolate-oxidizing enzyme; Mesostigma viride; Prasinophyceae
Strain(s): 296 
PubMed: 11038060
DOI: 10.1093/pcp/pcd020

Lemieux, C., Otis, C., Turmel, M. 2000 Ancestral chloroplast in Mesostigma viride reveals an early branch of green plant evolution. Nature, 403, 649-652.
Strain(s): 296 
PubMed: 10688199
DOI: 10.1038/35001059

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