Strain number NIES-107  
Phylum Cyanophyta  
Class Cyanophyceae  
Scientific name Microcystis aeruginosa (Kützing) Lemmermann  
Synonym Microcystis ichthyoblabe Kützing; Microcystis novacekii (Komárek) Compère; Microcystis viridis (A.Brown) Lemmermann; Microcystis wesenbergii Komárek  
Former name Microcystis wesenbergii Komárek  
Common name Blue-green alga ; Cyanobacteria  
Locality (Date of collection) Lake Kawaguchi, Yamanashi, Japan (1981-06-**)  
Latitude / Longitude 35.5130910551 / 138.748515637 
Habitat (Isolation source) Freshwater (Lake water)  
History < Watanabe, Makoto M.  
Isolator (Date of isolation) Watanabe, Michiko H. (1981-06-**)  
Identified by Watanabe, Michiko H.  
State of strain Cryopreservation; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  CBMA  
Temperature:  25 C
Light intensity:  13-16 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 16S rRNA ( U40333 DQ648028 ) , cpcB, cpcA ( DQ648023 ) , ftsZ ( AB324862 ) , glnA ( AB324941 ) , gltX ( AB325020 ) , gyrB ( AB325099 ) , mcyA ( AB110108 ) , mcyD ( AB110119 ) , mcyE ( AY817158 ) , mcyG ( AB110130 ) , mcyJ ( AB110141 ) , pgi ( AB325178 ) , pilT ( AY973320 ) , recA ( AB325257 ) , tpi ( AB325336 ) , RPB ( HM991460 )  
Cell size (min - max)  
Organization  
Characteristics Cyanobacterial water bloom (aoko) ; Toxicity ; microcystin (+) (Tanabe et al. 2009b) ; ST13 (Tanabe et al. 2007)  
Other strain no. Other strain no. : KW-MW-7  
Remarks Cryopreserved; Toxicity 
Movie  
Reference
Dulić, T., Svirčev, Z., Palanački Malešević, T., Faassen, E. J., Savela, H., Hao, Q., Meriluoto, J. 2022 Assessment of common cyanotoxins in cyanobacteria of biological loess crusts. Toxins, 14, 215 (article ID).
Keywords: cyanotoxins; biocrusts; loess; terrestrial cyanobacteria; sedimentary biosignatures; land restoration
Strain(s): 107 
PubMed: 35324712
DOI: 10.3390/toxins14030215

Spoof, L., Jaakkola, S., Važić, T., Häggqvist, K., Kirkkala, T., Ventelä, A. M., Kirkkala, T., Svirčev, Z., Meriluoto, J. 2020 Elimination of cyanobacteria and microcystins in irrigation water—effects of hydrogen peroxide treatment. Environ. Sci. Pollut. Res., 27, 8638–8652.
Keywords: Cyanobacteria; Microcystins; Irrigation water; Hydrogen peroxide; Spinach
Strain(s): 107 
PubMed: 31907814
DOI: 10.1007/s11356-019-07476-x

Tokodi, N., Drobac Backović, D., Lujić, J., Šćekić, I., Simić, S., Dordević, N., Dulić, T., Miljanović, N., Marinović, Z., Savela, H., Meriluoto, J., Svirčev, Z. 2020 Protected freshwater ecosystem with incessant cyanobacterial blooming awaiting a resolution. Water, 12, 129 (article ID).
Keywords: cyanobacteria; blooms; microcystin; Lake Ludoš
Strain(s): 107 
DOI: 10.3390/w12010129

Asukabe, H., Akahori, S., Ueno, E., Nakayama, T., Yamashita, R., Arii, S., Harada, K., Imanishi, S. Y. 2020 Cyanobacterial classification with the toxicity using MALDI Biotyper. J. Am. Soc. Mass Spectrom., 31, 1572-1578.
Keywords: cyanobacteria; MALDI Biotyper; microcystins; profiling
Strain(s): 27394273818789100101102103104105107111112207208230266298416433478510512515592593604611808809835843846847905928931932945981989102510431058106110641068106910711076108510991101110511131115112611331134113511441157121516421643165216681672167316771678169820872131241226653708373337343736375537563757 
PubMed: 32501712
DOI: 10.1021/jasms.0c00148

Dziga, D., Tokodi, N., Backović, D. D., Kokociński, M., Antosiak, A., Puchalski, J., Strzałka, W., Madej, M., Meriluoto, J., Svirčev, Z. 2019 The effect of a combined hydrogen peroxide-MlrA treatment on the phytoplankton community and microcystin concentrations in a mesocosm experiment in Lake Ludoš. Toxins, 11, 725 (article ID).
Keywords: microcystin; biodegradation; hydrogen peroxide; treatment; cyanobacteria; bloom
Strain(s): 107 
PubMed: 31835838
DOI: 10.3390/toxins11120725

González-Pleitera, M., Cirésa, S., Hurtado-Gallego, J., Leganés, F., Fernández-Piñas, F., Velázquez, D. 2019 Chapter 20 - Ecotoxicological assessment of antibiotics in freshwater using cyanobacteria. In Cyanobacteria: From Basic Science to Applications, Eds. by Mishra, A. K., Tiwari, D. N., Rai, A. N., Academic Press, pp. 339-417.
Keywords: Anabaena; Antibiotics; Biomarker; Bioreporter; Cyanobacteria; Ecotoxicology; Freshwater; Oxidative stress; Risk assessment
Strain(s): 192344107843 
DOI: 10.1016/B978-0-12-814667-5.00020-9

Tanabe, Y., Hodoki, Y., Sano, T., Tada, K., Watanabe, MM. 2018a Adaptation of the freshwater bloom-forming cyanobacterium Microcystis aeruginosa to brackish water is driven by recent horizontal transfer of sucrose genes. Front. Microbiol., 9, 1150 (article ID).
Keywords: Microcystis; bloom; salt tolerance; sucrose; genomics; brackish water; horizontal gene trasnfer; ecotype
Strain(s): 4487888990919899100101102103104105107108109112298299478604843901105010521059106310671069107010751076108510931094109811001101110211041105110611071108111311141115112211281130113111321134113911411142114311441150117011741175117611771179118111831185121112131214121512321234123812391252208820902091246524662467246824692470247124722473247524762477247824792480248124822483248424852486248724882489249024912492249424952546254725482549255025512552255325542555255625572558255925602561259225942595259625972598259926002601260226032604260526062607260826102611261226132617261826192620262126222623262426252626262826294234 
PubMed: 29922255
DOI: 10.3389/fmicb.2018.01150

Schlüter, L., David, G. S., Jørgensen, N. O. G., Podduturi, R., Tucci, A., Dias, A. S., da Silva, R. J. 2018 Characterization of phytoplankton by pigment analysis and the detection of toxic cyanobacteria in reservoirs with aquaculture production. Aquac. Environ. Interact., 10, 35-48.
Keywords: Microcystins; Pigment analysis; Cyanobacteria; Phytoplankton composition; HPLC; Fish farm
Strain(s): 107 
DOI: 10.3354/aei00256

Tokodi, N., Drobac, D., Meriluoto, J., Lujić, J., Marinović, Z., Važić, T., Nybom, S., Simeunović, J., Dulić, T., Lazić, G., Petrović, T., Vuković-Gačić, B., Sunjog, K., Kolarević, S., Kračun-Kolarević, M., Subakov-Simić, G., Miljanović, B., Codd, GA., Svirčev, Z. 2018 Cyanobacterial effects in Lake Ludoš, Serbia - Is preservation of adegraded aquatic ecosystem justified? Sci. Total Environ., 635, 635,
Keywords: Wetland; Cyanobacteria; Cyanotoxin; Eutrophication; Serbia
Strain(s): 107 
PubMed: 29710560
DOI: 10.1016/j.scitotenv.2018.04.177

Imanishi, S. Y., Nakayama, T., Asukabe, H., Harada, K. 2017 Application of MALDI Biotyper to cyanobacterial profiling. Rapid Commun. Mass Spectrom., 31, 325-332.
Strain(s): 738910210310711229861180880983584398910251043105810711099164216521672 
PubMed: 27862451
DOI: 10.1002/rcm.7793

Sun, L.W., Jiang, W.J., Sato, H., Kawachi, M., Lu, X.W. 2016 Rapid classification and identification of Microcystis aeruginosa strains using MALDI-TOF MS and polygenetic analysis. PLoS One, 11, e0156275 (article ID).
Strain(s): 44878889901001011041051071091111124786048439011043105310581061106810691070107210761077108510941099110111041105111311151126113411351144115111571170121112131215121612171218122212241230123212331239 
PubMed: 27227555
DOI: 10.1371/journal.pone.0156275

Anas, A. R. J., Nakajima, A., Naruse, C., Tone, M., Asukabe, H., Harada, K. 2016 Determination of fVIIa-sTF inhibitors in toxic Microcystis cyanobacteria by LC-MS technique. Mar. Drugs, 14, 7 (article ID).
Keywords: cyanobacteria; toxic Microcystis; anticoagulant; fVIIa-sTF inhibitors; peptides; aeruginosins; blood coagulation cascade
Strain(s): 891021031072981025104310581071108510991133 
PubMed: 26729138
DOI: 10.3390/md14010007

Hautala, H., Lamminmäki, U., Spoof, L., Nybom, S., Meriluoto, J., Vehniäinen, M. 2013 Quantitative PCR detection and improved sample preparation of microcystin-producing Anabaena, Microcystis and Planktothrix. Ecotox. Environ. Safe., 87, 49-56.
Keywords: Microcystin; Cyanobacteria; Quantitative PCR; mcyB; Sample prepararion
Strain(s): 107 
DOI: 10.1016/j.ecoenv.2012.10.008

Kataoka, T., Homma, T., Nakano, S., Hodoki, Y., Ohbayashi, K., Kondo, R. 2013 PCR primers for selective detection of intra-species variations in the bloom-forming cyanobacterium, Microcystis. Harmful Algae, 23, 46-54.
Keywords: Microcystis aeruginosa; Quantitative PCR; 16S-23S rDNA ITS fegion; Selective detection; Intra-species variation
Strain(s): 4487909810110210310711111229860410621070 
DOI: 10.1016/j.hal.2012.12.003

Tanabe, Y. & Watanabe, M. M. 2011 Local expansion of a panmictic lineage of water bloom-forming cyanobacterium Microcystis aeruginosa. PLoS One, 6, e17085 (article ID).
Strain(s): 4487888990919899100101102103104105106107108109110111112298299478604843901105010511052105310541055105610571058105910601061106210631064106510661067106810691070107210751076107710851090109110921093109410951096109710981100110111021103110411051106110711081113111411151122112311241125112611271128112911301131113211331134113511361137113811391140114111421143114411451146114711481149115011511152115311551156115711591160116111621163116411701171117211731174117511761177117911801181118311841185118612091211121312141215121612171218121912201221122212231224122512261227122812291230123112321233123412351236123712381239124012411242124312441245125112521253125420882089209020912465246624672468246924702471247224732474247524762477247824792480248124822483248424852486248724882489249024912492249324942495249625422543254425452546254725482549255025512552255325542555255625572558255925602561259125922593259425952596259725982599260026012602260326042605260626072608260926102611261226132617261826192620262126222623262426252626262726282629 
PubMed: 21390221
DOI: 10.1371/journal.pone.0017085

Sano, T., Takagi, H., Nagano, K., Nishikawa, M., Kaya, K. 2011 Accurate LC-MS analyses for microcystions using per-15N-labeled microcystins. Anal. Bioanal. Chem., 399, 2511-2516.
Keywords: Microcystin; Stable isotope; Surrogate; Liquid chromatography-mass spectrometry; Accurate analysis
Strain(s): 107 
PubMed: 21221535
DOI: 10.1007/s00216-010-4639-y

Spoof, L., Neffling, M.-R., Meriluoto, J. 2010 Fast separation of microcystins and nodularins on narrow-bore reversed-phase columns coupled to a conventional HPLC system. Toxicon, 55, 954-964.
Keywords: Microcystins; Nodularins; Fast high-performance liquid; chromatography
Strain(s): 107 
PubMed: 19540867
DOI: 10.1016/j.toxicon.2009.06.015

Tanabe, Y., Sano, T., Kasai, F., Watanabe, M. M. 2009b Recombination, cryptic clades and neutral molecular divergence of the microcystin synthetase (mcy) genes of toxic cyanobacterium Microcystis aeruginosa. BMC Evol. Biol., 9, 115 (article ID).
Strain(s): 44878889909198991001011021031041051061071081091101111122982994786048439011050105110521053105410551056105710581059106010611062106310641065106610671068106910701072107510761077108510901091109210931094109510961097109811001101110211031104110511061107110811131114111511221123112411251126112711281129113011311132113311341135113611371138113911401141114211431144114511461147114811491150115111521153115511561157115911601161116211631164117011711172120912111213121412151216121712181219122012211222122312241225122612271228122912301231123212331234123512361237123812391240124112421243124412451251125212531254246524662467246924702471247224732474247524762477247824792561 
PubMed: 19463155
DOI: 10.1186/1471-2148-9-115

Ha, K., Takamura, N., Jang, M.-H. 2009 Microcystin production by Microcystis aeruginosa exposed to phytoplanktivorous and omnivorous fish at different kairomone concentrations. Bull. Environ. Contam. Toxicol., 83, 761-765.
Keywords: microcystin; kairomone; Hypophthalmichthys molitrix; Carassius gibelio langsdorfi
Strain(s): 89103107 
PubMed: 19669611
DOI: 10.1007/s00128-009-9841-2

Jang, M.-H., Ha, K., Takamura, N. 2008 Microcystin production by Microcystis aeruginosa exposed to different stages of herbivorous zooplankton. Toxicon, 51, 882-889.
Keywords: Microcystis aeruginosa; induced defense; different stages of zooplankton; infochemicals; intracellular microcystin; extracellular microcystin
Strain(s): 8898103107 
PubMed: 18243271
DOI: 10.1016/j.toxicon.2007.12.015

Nybom, S. M. K., Salminen, S. J., Meriluoto, J. A. O. 2008 Specific strains of probiotic bacteria are efficient in removal of several different cyanobacterial toxins from solution. Toxicon, 52, 214-220.
Keywords: Cyanotoxin; Microcystin; Cylindrospermopsin; Probiotic bacteria; Bifidobacterium; Lactobacillus; Removal
Strain(s): 107 
PubMed: 18639912
DOI: 10.1016/j.toxicon.2008.04.169

Jang, M.-H., Ha, K., Takamura, N. 2007 Reciprocal allelopathic responses between toxic cyanobacteria (Microcystis aeruginosa) and duckweed (Lemna japonica). Toxicon, 49, 727-733.
Keywords: Microcystis aeruginosa Kützing; Lemna japonica Landolg; microcystin; allelochemicals; reciprocal allelopathic responses; growth; eutrophic waters
Strain(s): 103107 
PubMed: 17207510
DOI: 10.1016/j.toxicon.2006.11.017

Jang, M.-H., Jung, J.-M., Takamura, N. 2007 Changes in microcystin production in cyanobacteria exposed to zooplankton at different population densities and infochemical concentrations. Limnol. Oceanogr., 52, 1454-1466.
Strain(s): 8898107 
DOI: 10.4319/lo.2007.52.4.1454

Tanabe, Y., Kasai, F., Watanabe, M. M. 2007 Multilocus sequence typing (MLST) reveals high genetic diversity and clonal population structure of the toxic cyanobacterium Microcystis aeruginosa. Microbiology, 153, 3695-3703.
Strain(s): 44878889909198991001011021031041051061071081091101111122982994786048439019331050105110521053105410551056105710581059106010611062106310641065106610671068106910701072107510761077108510901091109210931094109510961097109811001101110211031104110511061107110811131114111511221123112411251126112711281129113011311132113311341135113611371138113911401141114211431144114511461147114811491150115111521153115511561157115911601161116211631164117011711172120912111213121412151216121712181219122012211222122312241225122612271228122912301231123212331234123512361237123812391240124112421243124412451251125212531254 
PubMed: 17975077
DOI: 10.1099/mic.0.2007/010645-0

Ando, T., Nagase, H., Eguchi, K., Hirooka, T., Nakamura, T., Miyamoto, K., Hirata, K. 2007 A novel method using cyanobacteria for ecotoxicity test of veterinary antimicrobial agents. Environ. Toxicol. Chem., 26, 601-606.
Keywords: Antimicrobial agent; Cyanobacterium; Ecotoxicity test; Algal toxicity
Strain(s): 1923441073277 
PubMed: 17447543
DOI: 10.1897/06-195R.1

Meriluoto, J., Codd, G. A. (eds.) 2005 TOXIC: cyanobacterial monitoring and cyanotoxin analysis. Acta Acad. Aboensis, ser, B, Vol. 65(1), 149 pp.
Strain(s): 107 

Karlsson, K. 2005 Liquid chromatotraphy - mass spectrometry in distribution and bioaccumulation studies of toxic cyanopeptides. PhD thesis, Åbo Akademi University, Turku, Finland, 62 pp.
Strain(s): 107 

Tanabe, Y., Kaya, K., Watanabe, M. M. 2004 Evidence for recombination in the microcystin synthetase (mcy) genes of toxic cyanobacteria Microcystis spp. J. Mol. Evol., 58, 633-641.
Keywords: cyanobacteria; microcystin synthetase; Microcystis; NRPS; recombination; split decomposition
Strain(s): 888990102103107 
PubMed: 15461420
DOI: 10.1007/s00239-004-2583-1

Meriluoto, J., Karlsson, K., Spoof, L. 2004 High-throughput screening of ten microcystins and nodularins, cyanobacterial peptide hepatotoxins, by reversed-phase liquid chromatography-electrospray ionization mass spectrometry. Chromatographia, 59, 291-298.
Keywords: columun liquid chromatography; mass spectrometry detection; microcystins and nodularins; cyanobacteria; toxins
Strain(s): 107 
DOI: 10.1365/s10337-003-0163-y

Spoof, L. 2004 High-performance liquid chromatography of microcystins and nodularins, cyanobacterial peptide toxins. PhD thesis, Åbo Akademi University, Turku, Finland, 73 pp.
Strain(s): 107 

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 192122232425262728293031323334353637383940414445465051525354555658596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123125127128129130131132133134135137138139144147151152153154155156157158159160162163164165166167168170171172173174175176177178179180181182183185186187188189191192193194197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268287288289290294295297298299300301302303305306308309310312313329334337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Tillett, D., Neilan, B. A. 2000 Xanthogenate nucleic acid isolation from cultured and environmental cyanobacteria. J. Phycol., 36, 251-258.
Keywords: cyanobacteria; DNA extraction; nucleic acids; PCR; xanthogenate
Strain(s): 81102107 
DOI: 10.1046/j.1529-8817.2000.99079.x

Neilan, B. A., Dittmann, E., Rouhiainen, L., Bass, R. A., Schaub, V., Sivonen, K., Börner, T. 1999 Nonribosomal peptide synthesis and toxigenicity of Cyanobacteria. J. Bacteriol., 181, 4089-4097.
Strain(s): 19248199102107204 
PubMed: 10383979
DOI: 10.1128/JB.181.13.4089-4097.1999

Kondo, R., Kagiya, G., Yuki, Y., Hiroishi, S., Watanabe, M. 1998 Taxonomy of a bloom-forming cyanobacterial genus Microcystis. Nippon Suisan Gakkaishi, 64, 291-292 (in Japanese with English title).
Strain(s): 87888990919899100101102104105106107108109110111112298299604 

Kondo, R., Komura, M., Hiroishi, S., Hata, Y. 1998 Detection and 16S rDNA sequence analysis of a bloom-forming cyanobacterial genus Microcystis. Fish. Sci., 64, 840-841.
Keywords: Microcystis; 16S rDNA; PCR
Strain(s): 107112 
DOI: 10.2331/fishsci.64.840

Neilan, B. A., Jacobs, D., Goodman, A. E. 1995 Genetic diversity and phylogeny of toxic cyanobacteria determined by DNA polymorphisms within the phycocyanin locus. Appl. Environ. Microbiol., 61, 3875-3883.
Strain(s): 244041447376788081878998102107111112204207298 
PubMed: 8526499

Neilan, B. A., Hawkins, P. R., Cox, P. T., Goodman, A. E. 1994 Towards a molecular taxonomy for the bloom-forming cyanobacteria. Aust. J. Mar. Freshwater Res., 45, 869-873.
Keywords: 16S rRNA; PCR; Microcystis; Anabaena; DNA sequence
Strain(s): 417889102107111 
DOI: 10.1071/MF9940869

Watanabe, M. M., Suda, S., Kasai, F., Sawaguchi, T. 1985 Axenic cultures of the three species of Microcystis (Cyanophyta = Cyanobacteria). Bull. JFCC, 1, 57-63.
Strain(s): 89102107111298 

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