Strain number NIES-564  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Astrephomene perforata Nozaki  
Synonym  
Former name  
Common name Green alga  
Locality (Date of collection) Hayama, Kanagawa, Japan (1980-12-04)  
Latitude / Longitude 35.271286 / 139.600715 
Habitat (Isolation source) Freshwater (Paddy soil)  
History < Nozaki, Hisayoshi  
Isolator (Date of isolation) Nozaki, Hisayoshi (1981-06-20)  
Identified by Nozaki, Hisayoshi  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  VTAC  
Temperature:  20 C
Light intensity:  3-5 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S rRNA ( LC086348 ) , atpB ( AB014024 ) , rbcL ( D63429 ) , psaA ( AB044238 ) , psaB ( AB044460 ) , psbC ( AB044518 AB044519 )  
Cell size (min - max)  
Organization Colonial; Flagellate 
Characteristics Phototaxis ; Authentic strain ; Heterothallic ; Isogamous ; Mating type (+) ; Crosses with NIES-565  
Other strain no. Other collection strain no. : UTEX 2474
Other strain no. : 1620-3-2  
Remarks Cryopreserved; Axenic 
Movie  
Reference
Nozaki, H., Mori, F., Tanaka, Y., Matsuzaki, R., Yamashita, S., Yamaguchi, H., Kawachi, K. 2023 Cryopreservation of two species of the multicellular volvocine green algal genus Astrephomene. BMC Microbiol., 23, 16 (article ID).
Keywords: Asexual cycle; Astrephomene; Cryopreservation; Cryoprotectant; Culture collection; Volvocine green algae
Strain(s): 4184195645656288538548554017 
PubMed: 36650459
DOI: 10.1186/s12866-023-02767-3

Lindsey, C. R., Rosenzweing, F., Herron, M. D. 2021 Phylotranscriptomics points to multiple independent origins of multicellularity and cellular differentiation in the volvocine algae. BMC Biol., 19, 182 (article ID).
Keywords: Phylotranscriptomics; Volvocine algae; Green algae; Phylogeny; Multicellularity
Strain(s): 41845645846056456656857265365471771972072573673785185689028633383 
DOI: 10.1101/2021.03.16.435725

Nakada, T., Tsuchida, Y., Tomita, M. 2019 Improved taxon sampling and multigene phylogeny of unicellular chlamydomonads closely related to the colonial volvocalean lineage Tetrabaenaceae-Goniaceae-Volvocaceae (Volvocales, Chlorophyceae). Mol. Phylogenet. Evol., 130 1-8.
Keywords: Alga; Chlamydomonas; Colony evolution; Multigene phylogeny; Vitreochlamys; Zoosporangium
Strain(s): 4184374564604745415445455645665695715776546667217257287357378678798808828838871362185824622567338333884241424242434244424542464247424842494250425142524253425442554256 
PubMed: 30266459
DOI: 10.1016/j.ympev.2018.09.013

Hanschen, E. R., Herron, M. D., Wiens, J. J., Nozaki, H., Michod, R. E. 2018 Repeated evolution and reversibilityof self-fertilization in the volvocinegreen algae. Evolution, 72, 386-398.
Keywords: Haploid; phylogenetics; self-fertilization; sex; sexual reproduction; volvocine green algae
Strain(s): 28941845645846054154354454556456656856957157257457858265373285185685787287587687787888088288389189210381362171117132457256927383382338837803782 
PubMed: 29134623
DOI: 10.1111/evo.13394

Hanschen, E. R., Herron, M. D., Wiens, J. J., Nozaki, H., Michod, R. E. 2018 Multicellularity drives the evolution of sexual traits. Am. Nat., 192, E93-E10.
Keywords: sex; volvocine green algae; multicellularity; ancestral state; reconstruction; anisogamy; sexual dimorphism
Strain(s): 28941842145645846052254154354454556456656856957157257457858265373275185185685787287587687787888088288389189210381362171117132457256927383382338837803782 
PubMed: 30125231
DOI: 10.1086/698301

Hanschen, E. R., Davison, D. R., Ferris, P. J., Michod, R. E. 2018 On the rediscovery of Volvox perglobator (Volvocales, Chlorophyceae) and the evolution of outcrossing from self-fertilization. Evol. Ecol. Res., 19, 299-318.
Keywords: homothallism; phylogenetics; reversion; self-fertilization; sexual reproduction; volvocine green algae
Strain(s): 28942145645846047452254154354454556456656856957157257457858265373275185185687287687787888088210381122114011481156115713621708171117131859245725692738338233883780378239864145425842594260 

Nakada, T., Ito, T., Tomita, M. 2016 18S ribosomal RNA gene phylogeny of a colonial volvocalean lineage (Tetrabanaceae-Goniaceae-Volvocaceae, Volvocales, Chlorophyceae) and its close relatives. J. Jpn. Bot., 91, 345-354.
Keywords: 18S rRNA; colonial; green algae; molecular phylogeny; unicellular; Volvocales
Strain(s): 4184564604745415445455645665716546667217257287357378678778798808818828831362224222462266256733884126 

Nakazawa, A. & Nishii, I. 2012 Amidic and acetonic cryoprotectants improve cryopreservation of volvocine green algae. CryoLetters, 33, 201-212.
Keywords: cryopreservation; dimethylformamide; hydroxyacetone; Volvocaceae; Volvox; Chlamydomonas
Strain(s): 4184685455645747177217287297307311331136117102236 
PubMed: 22825787

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 192122232425262728293031323334353637383940414445465051525354555658596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123125127128129130131132133134135137138139144147151152153154155156157158159160162163164165166167168170171172173174175176177178179180181182183185186187188189191192193194197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268287288289290294295297298299300301302303305306308309310312313329334337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Nozaki, H., Onishi, K., Morita, E. 2002 Differences in pyrenoid morphology are correlated with differences in the rbcL genes of members of the Chloromonas lineage (Volvocales, Chlorophyceae). J. Mol. Evol., 55, 414-430.
Keywords: ATP synthase β-subunit (atpB) gene; Chloromonas; gene evolution; large subunit of Rubisco (rbcL) gene; morphological evolution; P700 chlorophyll a-apoprotein A2 (psaB) gene; pyrenoid; Volvocales
Strain(s): 96123144213418421425428432439446447456474522564566567569571574582653732851858861875882883968 
PubMed: 12355262
DOI: 10.1007/s00239-002-2338-9

Nozaki, H., Misawa, K., Kajita, T., Kato, M., Nohara, S., Watanabe, M. M. 2000 Origin and evolution of the colonial Volvocales (Chlorophyceae) as inferred from multiple, chloroplast gene sequences. Mol. Phylogenet. Evol., 17, 256-268.
Strain(s): 418456460474541545564566569571572574577582653654666720721722724725727728730731732734735736737851854872882884886887888889890893895896898 
PubMed: 11083939
DOI: 10.1006/mpev.2000.0831

Nozaki, H., Ohta, N., Takano, H., Watanabe, M. M. 1999 Reexamination of phylogenetic relationships within the colonial Volvocales (Chlorophyta): an analysis of atpB and rbcL gene sequences. J. Phycol., 35, 104-112.
Keywords: atpB gene; Chlorophyta; Gonium; molecular phylogeny; rbcL gene; Volvocaceae; volvocine series; Volvox
Strain(s): 418456460541564566568569571572574577582653654666720721722724725727728730731732734735736737851852858861882884895 
DOI: 10.1046/j.1529-8817.1999.3510104.x

Nozaki, H., Ito, M., Sano, R., Uchida, H., Watanabe, M. M., Kuroiwa, T. 1995 Phylogenetic relationships within the colonial Volvocales (Chlorophyta) inferred from rbcL gene sequence data. J. Phycol., 31, 970-979.
Keywords: Chlorophyta; Goniaceae; large subunit of ribulose-1,5-bisphosphate carboxylase / oxygenase (rbcL) gene; molecular phylogeny; Tetrabaenaceae; Volvocaceae
Strain(s): 418421456460541564566569571572574577653725731732734735737895 
DOI: 10.1111/j.0022-3646.1995.00970.x

Nozaki, H. 1983 Morphology and taxonomy of two species of Astrephomene (Chlorophyta, Volvocales) in Japan. J. Jpn. Bot., 58, 345-352, pls. 11-13.
Strain(s): 418419564565 

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