A big image opens when the thumbnail is clicked.(nies-0446.jpg)
Strain number NIES-446  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Chlamydomonas tetragama (Bohlin) Wille  
Synonym Chlorogonium tetragamum Bohlin  
Former name Chlorogonium metamorphum Skuja  
Common name Green alga  
Locality (Date of collection) Tsuchiura, Ibaraki, Japan (1985-04-13)  
Latitude / Longitude 36.081096 / 140.16337 
Habitat (Isolation source) Freshwater (Pond water)  
History < Suda, Shoichiro  
Isolator (Date of isolation) Suda, Shoichiro (1985-04-13)  
Identified by Suda, Shoichiro  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2017 Dec]  
Culture condition
(Preculture condition)
Medium:  C  
Temperature:  20 C
Light intensity:  10-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M  
Gene information 5.8S rRNA ( AB004866 ) , 18S rRNA ( AB007370 ) , atpB ( AB084319 ) , psaB ( AB084357 ) , rbcL ( AJ001880 AB007835 )  
Cell size (min - max) - 19 μm  
Organization Unicellular; Flagellate 
Characteristics Phototaxis ; Authentic strain, Neotype (Nozaki and Watanabe 1996)  
Other strain no. Other strain no. : 413D4-4  
Remarks Cryopreserved; Axenic 
Movie  
Reference
Jeong, S.-Y., Son, H.-J. 2021 Effects of mycosubtilin homolog algicides from a marine bacterium, Bacillus sp. SY-1, against the harmful algal bloom species Cochlodinium polykrikoides. J. Microbiol., 59, 389-400.
Keywords: algicide; Bacillus sp. SY-1; Cochlodinium polykrikoides; harmful algal bloom; mycosubtilin
Strain(s): 23227238298316446682 
PubMed: 33779952
DOI: 10.1007/s12275-021-1086-8

Munakata, H., Nakada, T., Nakahigashi, K., Nozaki, H., Tomita, M. 2016 Phylogenetic position and molecular chronology of a colonial green flagellate, Stephanosphaera pluvialis (Volvocales, Chlorophyceae), among unicellular algae. J. Eucaryot. Microbiol., 63, 340-348.
Keywords: Balticola; coloniality; divergence time; evolution; molecular phylogeny
Strain(s): 12314442142843243944656756957165686188796810482293256441244125 
PubMed: 26595722
DOI: 10.1111/jeu.12283

Luo, W., Kotut, K., Krienits, L. 2013 Hidden diversity of eukaryotic plankton in the soda lake Nakuru, Kenya, during a phase of low salinity revealed by a SSU rRNA gene clone library. Hydrobiologia, 702, 95-103.
Keywords: Chlorophyta; clone library; lake Nakuru; lesser flamingo; phytoflagellates; Soda lakes; SSU rRNA
Strain(s): 4461360 
DOI: 10.1007/s10750-012-1310-y

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Nozaki, H., Onishi, K., Morita, E. 2002 Differences in pyrenoid morphology are correlated with differences in the rbcL genes of members of the Chloromonas lineage (Volvocales, Chlorophyceae). J. Mol. Evol., 55, 414-430.
Keywords: ATP synthase β-subunit (atpB) gene; Chloromonas; gene evolution; large subunit of Rubisco (rbcL) gene; morphological evolution; P700 chlorophyll a-apoprotein A2 (psaB) gene; pyrenoid; Volvocales
Strain(s): 96123144213418421425428432439446447456474522564566567569571574582653732851858861875882883968 
PubMed: 12355262
DOI: 10.1007/s00239-002-2338-9

Kodani, S., Imoto, A., Mitsutani, A., Murakami, M. 2002 Isolation and identification of the antialgal compound, harmane (1-methyl-B-carboline), produced by the algicidal bacterium, Pseudomonas sp. K44-1. J. Appl. Phycol., 14, 109-114.
Keywords: Algicidal bacteria; Antialgal compound; Cyanobacteria; Harmane; Pseudomonas sp.; Water bloom
Strain(s): 1923102227299446506 
DOI: 10.1023/A:1019533414018

Hepperle, D., Nozaki, H., Hohenberger, S., Huss, V. A. R., Morita, E., Krienitz, L. 1998 Phylogenetic position of the Phacotaceae within the Chlamydophyceae as revealed by analysis of 18S rDNA and rbcL sequences. J. Mol. Evol., 47, 420-430.
Keywords: Phacotaceae; Volvocales; 18S rDNA phylogeny; lorica-bearing flagellates; green algae
Strain(s): 446858859861862 
PubMed: 9767687
DOI: 10.1007/PL00006399

Nozaki, H., Ohta, N., Morita, E., Watanabe, M. M. 1998 Toward a natural system of species in Chlorogonium (Volvocales, Chlorophyta): a combined analysis of morphological and rbcL gene sequence data. J. Phycol., 34, 1024-1037.
Keywords: Chlorogonium; Chlorogonium kasakii sp. nov.; Chlorophyta; molecular phylogeny; monophyletic species; morphology; natural systematics; rbcL gene; species taxonomy; Volvocales
Strain(s): 123439446692742743744745746747748749750751752753754755756757758759760761 
DOI: 10.1046/j.1529-8817.1998.341024.x

Uchida, H., Kitae, K., Tomizawa, K., Yokota, A. 1998 Comparison of the nucleotide sequence and secondary structure of the 5.8S ribosomal RNA gene of Chlamydomonas tetragama with those of green algae. DNA Seq., 8, 403-408.
Keywords: 5.8S ribosomal RNA gene; Chlamydomonas
Strain(s): 446 
PubMed: 10728826
DOI: 10.3109/10425179809020902

Nozaki, H., Aizawa, K., Watanabe, M. M. 1996 Re-examination of two NIES strains labeled Chlorogonium metamorphum (Volvocales, Chlorophyta) from Japan. Nova Hedwigia Beiheft, 112, 483-490.
Keywords: Chlorogonium; Chlorophyta; morphology; neotype strain; species identification; Volvocales
Strain(s): 123446 

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