Strain number NIES-4033  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Carolibrandtia ciliaticola (Hoshina, Kobayashi, Suzaki & Kusuoka) Hoshina & Nakada  
Synonym Brandtia ciliaticola Hoshina, Kobayashi, Suzaki & Kusuoka  
Former name  
Common name Green alga  
Locality (Date of collection) Lake Biwa, Oroshimo, Kusatsu, Shiga, Japan (2015-12-22)  
Latitude / Longitude 35.0741068 / 135.9317646 
Habitat (Isolation source) Freshwater (Pelagodileptus trachelioides)  
History < Hoshina, Ryo  
Isolator (Date of isolation) Hoshina, Ryo (2016-05-30)  
Identified by Hoshina, Ryo  
State of strain Cryopreservation; Unialgal; Non-axenic[2017 May]  
Culture condition
(Preculture condition)
Medium:  CAFAC  
Temperature:  20 C
Light intensity:  14-18 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M  
Gene information 18S-ITS1-5.8S-ITS2 ( LC228604 )  
Cell size (min - max) 3 - 5 μm  
Organization  
Characteristics Symbiotic ; Authentic strain (Hoshina et al. 2018) ; Type specimen (NIES-50018, Holotype)  
Other strain no. Other strain no. : 1224PelagoG  
Remarks Cryopreserved 
Movie  
Reference
Pröschold, T., Pitsch, G., Darienko, T. 2020 Micractinium tetrahymenae (Trebouxiophyceae, Chlorophyta), a new endosymbiont isolated from ciliates. Diversity-Basel, 12, 200 (article ID).
Keywords: Micractinium tetrahymenae; Tetrahymena; Utricularia; facultative endosymbiosis; ciliate-algae symbiosis
Strain(s): 216721714033 
DOI: 10.3390/d12050200

Pröschold, T. & Darienko, T. 2020 Choricystis and Lewiniosphaera gen. nov. (Trebouxiophyceae Chlorophyta), two different green algal endosymbionts in freshwater sponges. Symbiosis, 82, 175-188.
Keywords: Choricystis; Lewiniosphaera; Freshwater sponges; Endosymbiosis
Strain(s): 21672171234240334292 
PubMed: 33328698
DOI: 10.1007/s13199-020-00711-x

Hoshina, R., Kobayashi, M., Suzaki, T., Kusuoka, Y. 2018 Brandtia ciliaticola gen. et sp. nov. (Chlorellaceae, Trebouxiophyceae) a common symbiotic green coccoid of various ciliate species. Phycol. Res., 66, 76-81.
Keywords: Chlorella; genus definition; rRNA structure
Strain(s): 403240334034 
DOI: 10.1111/pre.12194

Hoshina, R. & Nakada, T. 2018 Carolibrandtia nom. nov. as a replacement name for Brandtia Hoshina (Chlorellaceae, Trebouxiophyceae). Phycol. Res., 66, 82-83.
Keywords: Chlorella-like alga; ciliate; later homonym; nomenclature; photobiont; symbiosis
Strain(s): 403240334034 
DOI: 10.1111/pre.12208

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