Strain number NIES-2242  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Chlamydomonas sphaeroides Gerloff  
Synonym  
Former name Chlamydomonas iyengari Mitra  
Common name Green alga  
Locality (Date of collection) India  
Latitude / Longitude  
Habitat (Isolation source)  
History < IAM (2007) < BIU (UTEX; 1967);  
Isolator (Date of isolation) Pringsheim, E. G.  
Identified by  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 C
Light intensity:  2-3 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  6 M  
Gene information Whole-genome ( BDDC00000000 ) , 18S rRNA ( AB701556 ) , EPT ( LC068986 ) , ECT ( LC068985 ) , EKI ( LC068805 )  
Cell size (min - max) 8 - 12 μm  
Organization Unicellular; Flagellate 
Characteristics Phototaxis ; Genome decoded strain (Hirashima et al. 2016)  
Other strain no. Other collection strain no. : IAM C-255; CCAP 11/14; UTEX 221; SAG 25.72; CGC CC-1811
 
Remarks Cryopreserved; Axenic 
Movie  
Reference
Matsuzaki, R., Suzuki, S., Yamaguchi, H., Kawachi, M., Kanesaki, Y., Yoshikawa, H., Mori, T., Nozaki, H. 2021 The Rubisco small subunits in the green algal genus Chloromonas provide insights into evolutionary loss of the eukaryotic carbon-concentrating organelle, the pyrenoid. BMC Ecol. Evol., 21, 11 (article ID).
Keywords: Chloromonas; Evolution; Green algae; Hydrophobicity of RBCS helices; Pyrenoid; Pyrenoid loss; Rubisco small subunit (RBCS)
Strain(s): 14442442522072212221522422243249937534284 
PubMed: 33514317
DOI: 10.1186/s12862-020-01733-1

Hirashima, T., Toyoshima, M., Moriyama, T., Sato, N. 2018 Evolution of the phosphatidylcholine biosynthesis pathways in green algae: Combinatorial diversity of methyltransferases. J. Mol. Evol., 86, 68-76.
Keywords: Chlamydomonas; Metabolic diversity; Phosphatidylcholine biosynthesis; Phosphoethanolamine-N-methyltransferase; Phospholipid-N-methyltransferase
Strain(s): 2202220722122242 
PubMed: 29330556
DOI: 10.1007/s00239-017-9826-4

Hirashima, T., Toyoshima, M., Moriyama, T., Nakamura, Y., Sato, N. 2017 Characterization of phosphoethanolamine-N-methyltransferases in green algae. Biochem. Biophys. Res. Commun., 488, 141-146.
Keywords: Green algal evolution; Chlamydomonas; Phosphatidylcholine biosynthesis; Phosphoethanolamine-N-methyltransferase
Strain(s): 22022207221222352242 
PubMed: 2848352
DOI: 10.1016/j.bbrc.2017.05.026

Hirashima, T., Tajima, N., Sato, N. 2016 Draft genome sequences of four species of Chlamydomonas containing phosphatidylcholine. GenomeA, 4, e01070-16 (article ID).
Strain(s): 2202220722122242 
PubMed: 27688324
DOI: 10.1128/genomeA.01070-16

Sato, N., Mori, N., Hirashima, T., Moriyama, T. 2016 Diverse pathways of phosphatidylcholine biosynthesis in algae as estimated by labeling studies and genomic sequence analysis. Plant J., 87, 281–292.
Keywords: Chlamydomonas; Cyanidioschyzon merolae; phosphatidylcholine biosynthesis; phosphatidylethanolamine methyltransferase; phosphoethanolamine methyltransferase
Strain(s): 22072242 
PubMed: 27133435
DOI: 10.1111/tpj.13199

Nakada, T., Ito, T., Tomita, M. 2016 18S ribosomal RNA gene phylogeny of a colonial volvocalean lineage (Tetrabanaceae-Goniaceae-Volvocaceae, Volvocales, Chlorophyceae) and its close relatives. J. Jpn. Bot., 91, 345-354.
Keywords: 18S rRNA; colonial; green algae; molecular phylogeny; unicellular; Volvocales
Strain(s): 4184564604745415445455645665716546667217257287357378678778798808818828831362224222462266256733884126 

Sakurai, K., Mori, N., Sato, N. 2014 Detection and characterization of phosphatidylcholine in various strains of the genus Chlamydomonas (Volvocales, Chlorophyceae). J. Plant Res., 127, 641-650.
Keywords: Chlamydomonas; Delta 5 desaturase; Diacylglyceryl-N,N,N-trimethylhomoserine; Fatty acid; Phosphatidylcholine
Strain(s): 22022207221222152219222522302242231624622499 
PubMed: 24947506
DOI: 10.1007/s10265-014-0644-0

Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12.
Strain(s): 158437438440884968102110221048173318481849220122022203220422062207220822092210221122122213221422152216221822192220222222232224222522282229223022312233223422352236223722382239224022412242224322462247224823142315231623172318231923202321232223232324246224632499257525762577257825793284 

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