BESM | BESM 2 | ESM | ESM + CB–V | ESM2 | f/2 | f/2 + NH4Cl |
IMK | IMK + NaCl | IMK + Soil Extract | IMK/2 | K | K/2 | K/2ET |
M–ASP7 | MB | MF | MKM | MNK | Modified IMR ( mIMR ) | Modified SWM–3 (mSWM–3) |
PRO–99 | WESM |
B–I | B–II | B–III | B–IV | B–V | YT |
Bf/2 | MM 23 | STP |
Bold 3N |
mSWC–2 ( Modified SWC–2 ) | SWC–1 | SWCN–1 | SWCN–2 | SWCN–3 | SWCN–4 | 1/3 Herbst ASW |
1 Media for freshwater, terrestrial, hot spring and salt water algae
Prepare as for AF–6 medium but adjust to pH 5.5–5.8.
1 Media for freshwater, terrestrial, hot spring and salt water algae
Dilute CSi 1 ) medium with distilled
water to one–fifth. Adjust to pH 3 with sulfuric acid.
1 ) See CSi
1 Media for freshwater, terrestrial, hot spring and salt water algae
NaNO3 | 14 mg |
NH4NO3 | 2.2 mg |
MgSO4 · 7H2O | 3 mg |
KH2PO4 | 1 mg |
K2HPO4 | 0.5 mg |
CaCl2 · 2H2O | 1 mg |
CaCO3 1 ) | 1 mg |
Fe–citrate | 0.2 mg |
Citric acid | 0.2 mg |
Biotin | 0.2 μg |
Thiamine HCl | 1 μg |
Vitamin B6 | 0.1 μg |
Vitamin B12 | 0.1 μg |
Trace metals 1 ) | 0.5 mL |
Distilled water | 99.5 mL |
pH 6.6 2 ) |
1 ) In the NIES–Collection, CaCO3 is removed and PIV metals
are used instead of Trace metals.
2 ) In the NIES–Collection, 40 mg MES is added and pH is adjusted to 6.6.
Reference(s) |
---|
Kato, S. 1982 Laboratory culture and morphology of Colacium vesiculosum Ehrb. ( Euglenophyceae ). Jpn. J. Phycol., 30, 63-67 ( in Japanese with English summary ). |
1 Media for freshwater, terrestrial, hot spring and salt water algae
AF–6 1 )
medium is diluted with distilled water to one-half.
1 ) See AF–6
1 Media for freshwater, terrestrial, hot spring and salt water algae
Mix 1 volume of AF–6
1 ) medium and 2 volumes of distilled water.
1 ) See AF–6
1 Media for freshwater, terrestrial, hot spring and salt water algae
To 100 mL AF–6
1 ) medium add 20 mg sodium acetate.
1 ) See AF–6
1 Media for freshwater, terrestrial, hot spring and salt water algae
( NH4 )2SO4 | 132 mg |
KH2PO4 | 27.2 mg |
MgSO4 · 7H2O | 24.6 mg |
CaCl2 · 2H2O | 7.4 mg |
Allen metals 1 ) | 0.01 mL |
Distilled water | 99.9 mL |
pH 2.5 2 ) |
1 ) See Allen metals
2 ) pH is adjusted to 2.5 with 0.5 mol / L H2SO4.
Reference(s) |
---|
Allen, M. B. 1959 Studies with Cyanidium caldarium, an anomalously pigmented chlorophyte. Arch. Mikrobiol., 32, 270-277. |
Starr, R. C., Zeikus, J. A. 1987 UTEX - The culture collection of algae at the University of Texas at Austin. J. Phycol., 23, Suppl. to Sept ., 1-106. |
1 Media for freshwater, terrestrial, hot spring and salt water algae
NaNO3 | 25 mg |
KH2PO4 | 17.5 mg |
K2HPO4 | 10 mg |
MgSO4 · 7H2O | 7.5 mg |
CaCl2 · 2H2O | 2.5 mg |
NaCl | 2.5 mg |
KOH | 3.1 mg |
FeSO4 · 7H2O | 0.498 mg |
H3BO3 | 1.142 mg |
ZnSO4 · 7H2O | 0.882 mg |
MnCl2 · 4H2O | 0.144 mg |
MoO3 | 0.071 mg |
CuSO4 · 5H2O | 0.157 mg |
Co( NO3 )2 · 6H2O | 0.049 mg |
Na2EDTA | 5 mg |
Distilled water | 100 mL |
Add 1.2 g agar to 100 mL of medium to give a solid medium.
Reference(s) |
---|
Bischoff, H. W., Bold, H. C. 1963 Some soil algae from enchanted rock and related algal species. Phycological Studies IV.,Univ. No. 6318, Texas, p. 95. |
1 Media for freshwater, terrestrial, hot spring and salt water algae
NaNO3 | 150 mg |
K2HPO4 · 3H2O | 4 mg |
MgSO4 · 7H2O | 7.5 mg |
CaCl2 · 2H2O | 3.6 mg |
Citric acid | 0.6 mg |
Ferric ammonium citrate | 0.6 mg |
Na2EDTA – Mg | 0.1 mg |
Na2CO3 | 2 mg |
Trace metal mix A5 + Co 1 ) | 0.1 mL |
Distilled water | 99.9 mL |
pH 7.4 |
Add 1.5 g agar to 100 mL of medium to give a solid medium.
1 ) See Trace metal mix A5 + Co
Reference(s) |
---|
Waterbury, J. B., Sranier, R. Y. 1981 Isolation and growth of cyanobacteria from marine and hypersaline environments. In The Prokaryotes, Springer Berlin Heidelberg, New York, pp. 221-223. |
1 Media for freshwater, terrestrial, hot spring and salt water algae
Ca( NO3 )2 · 4H2O | 15 mg |
KNO3 | 10 mg |
β–Na2glycerophosphate · 5H2O | 5 mg |
MgSO4 · 7H2O | 4 mg |
Vitamin B12 | 0.01 μg |
Biotin | 0.01 μg |
Thiamine HCl | 1 μg |
PIV metals 1 ) | 0.3 mL |
Tris ( hydroxymethyl ) aminomethane | 50 mg |
Distilled water | 99.7 mL |
pH 7.5 |
Add 1.5 g agar to 100 mL of medium to give a solid medium.
1 ) See PIV metals
Reference(s) |
---|
Ichimura, T. 1971 Sexual cell division and conjugation-papilla formation in sexual reproduction of Closterium strigosum. In Proceedings of the Seventh International Seaweed Symposium, University of Tokyo Press, Tokyo, p. 208-214. |
1 Media for freshwater, terrestrial, hot spring and salt water algae
C 1 ) medium with 10% filtered seawater.
1 ) See C
1 Media for freshwater, terrestrial, hot spring and salt water algae
Mix 1 volume of C 1 ) medium and 5 volumes of
distilled water.
1 ) See C
1 Media for freshwater, terrestrial, hot spring and salt water algae
Mix 1 volume of C 1 ) medium and 5 volumes of
Lake Nojiri water ( sterilized through GF/F filter, and store at 5°C ).
1 ) See C
1 Media for freshwater, terrestrial, hot spring and salt water algae
Mix 1 volume of C 1 ) medium and 9 volumes of
distilled water.
1 ) See C
1 Media for freshwater, terrestrial, hot spring and salt water algae
Ca( NO3 )2 · 4H2O | 2 mg |
KNO3 | 10 mg |
NH4NO3 | 5 mg |
β–Na2glycerophosphate · 5H2O | 3 mg |
MgSO4 · 7H2O | 2 mg |
Vitamin B12 | 0.01 μg |
Biotin | 0.01 μg |
Thiamine HCl | 1 μg |
PIV metals 1 ) | 0.1 mL |
Fe ( as EDTA; 1:1 molar ) 2 ) | 0.1 mL |
HEPES | 40 mg |
Distilled water | 99.9 mL |
pH 7.2 |
1 ) See PIV metals
2 ) See Fe ( as EDTA; 1:1 molar )
Reference(s) |
---|
Ichimura, T., Watanabe, M. 1974 The Closterium calosporum complex from the Ryukyu Islands - Variation and taxonomical problems. Mem. Natn. Sci. Mus. Tokyo, 7, 89-102, pls. 13-14. |
1 Media for freshwater, terrestrial, hot spring and salt water algae
CA 1 ) medium with pH adjusted to 6.5 by buffering
with MES instead of HEPES.
1 ) See CA
1 Media for freshwater, terrestrial, hot spring and salt water algae
NaNO3 | 24.7 mg |
CaCl2 · 2H2O | 1.1 mg |
MgSO4 · 7H2O | 4.7 mg |
K2HPO4 | 0.9 mg |
KH2PO4 | 2.3 mg |
NaCl | 1.5 mg |
PIV metals 1 ) | 0.5 mL |
Distilled water | 99.5 mL |
pH 7.5 |
In the NIES–Collection, 0.02 μg vitamin B12, 0.02 μg biotin and
2 μg thiamine HCl are added to this medium.
1 ) See PIV metals
Reference(s) |
---|
Carefoot, J. R. 1968 Culture and heterotrophy of the freshwater dinoflagellate, Peridinium cinctum fa. ovoplanum Lindeman. J. Phycol., 4, 129-131. |
1 Media for freshwater, terrestrial, hot spring and salt water algae
To C 1 ) medium add Bicine instead of Tris (
hydroxymethyl ) aminomethane, and adjust pH to 9.0.
1 ) See C
1 Media for freshwater, terrestrial, hot spring and salt water algae
Make B–V 1 ) medium with C
2 ) medium.
1 ) See B–V
2 ) See C
1 Media for freshwater, terrestrial, hot spring and salt water algae
C 1 ) medium with pH adjusted to 3.0 by buffering
with 1,2,3,4–cyclopentane tetracarboxylic acid instead of Tris ( hydroxymethyl
) aminomethane.
1 ) See C
Reference(s) |
---|
Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae ( I ) [17. Bisaisôrui no hozonhô ( I )]. In Preservation methods of microorganisms [ Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 ( in Japanese without English title ). |
1 Media for freshwater, terrestrial, hot spring and salt water algae
C 1 ) medium with pH adjusted to 7.0 by buffering
with 50mg HEPES instead of Tris ( hydroxymethyl ) aminomethane. Thereafter,
10 mg Na2SiO3 · 9H2O is added.
1 ) See C
1 Media for freshwater, terrestrial, hot spring and salt water algae
To 100 mL CSi 1 ) medium add 0.25 mg CuSO4
· 5H2O and 100 mg agar.
1 ) See CSi
1 Media for freshwater, terrestrial, hot spring and salt water algae
Dilute CSi 1 ) medium with distilled water
to 1/5.
1 ) See CSi
1 Media for freshwater, terrestrial, hot spring and salt water algae
C 1 ) medium with pH adjusted to 8.2 by buffering
with 40mg TAPS instead of Tris ( hydroxymethyl ) aminomethane.
1 ) See C
Reference(s) |
---|
Watanabe, M. M., Ichimura, T. 1977 Fresh- and salt-water forms of Spirulina platensis in axenic cultures. Bull. Jpn. Soc. Phycol., 25, Suppl. ( Mem. Iss. Yamada ) , 371-377. |
1 Media for freshwater, terrestrial, hot spring and salt water algae
To 100 mL C 1 ) medium add 100 mg yeast extract
and 200 mg tryptone.
1 ) See C
1 Media for freshwater, terrestrial, hot spring and salt water algae
D stock medium 1 ) | 5 mL |
HEPES | 0.12 mg |
FeCl3 · 6H2O | 1.14 mg |
Distilled water | 95 mL |
pH 8.24 – 8.26 |
After autoclaving, keep in room temperature overnight. Next day, adjust
pH to 7.5–7.6 and add 1.5 g agar.
1 ) See D stock medium
1 Media for freshwater, terrestrial, hot spring and salt water algae
MES | 20 mg |
MgSO4 · 7H2O | 5 mg |
KCl | 0.3 mg |
NH4Cl | 0.27 mg |
NaNO3 | 2 mg |
β–Na2glycerophosphate · 5H2O | 0.22 mg |
H3BO3 | 0.08 mg |
Na2EDTA · 2H2O | 0.8 mg |
Na2SiO3 · 9H2O | 1.4 mg |
FeCl3 · 6H2O | 0.1 mg |
CaCl2 · 2H2O | 7.5 mg |
Vitamin B12 | 0.05 μg |
Biotin | 0.05 μg |
Thiamine HCl | 10 μg |
DY trace metal solution 1 ) | 0.1 mL |
Distilled water | 99.9 mL |
pH 6.8 |
1 ) See DY trace metal solution
1 Media for freshwater, terrestrial, hot spring and salt water algae
KH2PO4 | 2 mg |
MgSO4 · 7H2O | 2.5 mg |
Sodium acetate | 40 mg |
Potassium citrate | 4 mg |
Polypeptone | 60 mg |
Yeast extract | 40 mg |
Vitamin B12 | 0.05 μg |
Thiamine HCl | 0.04 mg |
Distilled water | 100 mL |
pH 6.4 |
Add 150 mg agar to 100 mL of medium to give a semi-solid medium.
Reference(s) |
---|
Ichimura, T. 1979 2. Isolation and culture methods of algae. 2.5.B. Freshwater algae [2. Sôrui no bunri to baiyôhô. 2.5.B. Tansui sôrui]. In Methods in Phycological Studies [Sôrui Kenkyûhô], Eds. by Nishizawa, K. & Chihara, M., Kyoritsu Shuppan, Tokyo, p. 301 (in Japanese without English title). |
1 Media for freshwater, terrestrial, hot spring and salt water algae
NaNO3 | 10 mg |
K2HPO4 | 1 mg |
MgSO4 · 7H2O | 7.5 mg |
CaCl2 · 2H2O | 4 mg |
Na2CO3 | 3 mg |
FeSO4 · 7H2O | 0.1 mg |
Na2EDTA · 2H2O | 0.1 mg |
Distilled water | 100 mL |
pH 8.0 |
Reference(s) |
---|
Hagiwara, T., Yagi, O., Takamura, Y., Sudo, R. 1984 Isolation of bacteria-free Microcystis aeruginosa from Lake Kasumigaura. Jpn. J. Water Poll. Res., 7, 437-442 ( in Japanese with English summary ). |
Yagi, O., Okada, M., Sudo, R. 1979 Cultivation of Microcystis and red-tide-organisms. Res. Rep. Natl. Inst. Environ. Stud., No. 6, 223-229 ( in Japanese with English summary ). |
1 Media for freshwater, terrestrial, hot spring and salt water algae
Ca( NO3 )2 · 4H2O | 5 mg |
KNO3 | 10 mg |
NaNO3 | 5 mg |
Na2SO4 | 4 mg |
MgCl2 · 6H2O | 5 mg |
β–Na2glycerophosphate · 5H2O | 10 mg |
Na2EDTA · 2H2O | 0.5 mg |
FeCl3 · 6H2O | 0.05 mg |
MnCl2 · 4H2O | 0.5 mg |
ZnCl2 | 0.05 mg |
CoCl2 · 6H2O | 0.5 mg |
Na2MoO4 · 2H2O | 0.08 mg |
H3BO3 | 2 mg |
Bicine | 50 mg |
Distilled water | 100 mL |
pH 8.6 |
Reference(s) |
---|
Ichimura, T. 1979 2. Isolation and culture methods of algae. 2.5.B. Freshwater algae [2. Sôrui no bunri to baiyôhô. 2.5.B. Tansui sôrui]. In Methods in Phycological Studies [Sôrui Kenkyûhô], Eds. by Nishizawa, K. & Chihara, M., Kyoritsu Shuppan, Tokyo, p. 294-305 ( in Japanese without English title ). |
1 Media for freshwater, terrestrial, hot spring and salt water algae
To 100 mL of AF–6 1 ) medium add 10 mg glucose
and 10 mg sodium acetate.
1 ) See AF–6
1 Media for freshwater, terrestrial, hot spring and salt water algae
( NH4 )2SO4 | 262 mg |
KH2PO4 | 54 mg |
MgSO4 · 7H2O | 50 mg |
CaCl2 · 2H2O | 14 mg |
A2 trace element stock solution 1 ) | 0.2 mL |
Distilled water | 99.4 mL |
pH 2.5 2 ) |
After autoclaving, add 0.4 mL of A2 Fe stock solution
3 ) ( filter-sterilized ).
1 ) See A2 trace element stock solution
2 ) pH is adjusted to 2.5 with 0.5 mol / L H2SO4.
3 ) See A2 Fe stock solution
Indicated as “MA” medium in reference.
Reference(s) |
---|
Minoda, A., Sakagami, R., Yagisawa, F., Kuroiwa, T., Tanaka, K. 2004 Improvement of culture conditions and evidence for nuclear transformation by homologous recombination in a red alga, Cyanidioschyzon merolae 10D. Plant Cell Physiol., 45, 667-671. |
1 Media for freshwater, terrestrial, hot spring and salt water algae
To 100 mL M–Allen 1 ) medium add 0.5 –1 g Glucose.
1 ) See M–Allen
1 Media for freshwater, terrestrial, hot spring and salt water algae
To 100 mL M–Allen 1 ) medium add 50 mg
uracil.
1 ) See M–Allen
1 Media for freshwater, terrestrial, hot spring and salt water algae
KNO3 | 25 mg |
MgSO4 · 7H2O | 7.5 mg |
K2HPO4 | 7.5 mg |
KH2PO4 | 17.5 mg |
NaCl | 2.5 mg |
CaCl2 · 2H2O | 1 mg |
Fe solution 1 ) | 0.1 mL |
A5 solution 2 ) | 0.1 mL |
Agar | 1.5 g |
Distilled water | 99.8 mL |
pH 6.0 |
1 ) See Fe solution.
2 ) See A5 solution .
Indicated as “Modified Bristol medium” in reference.
Reference(s) |
---|
Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae ( I ) [17. Bisaisôrui no hozonhô ( I )]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 ( in Japanese without English title ). |
1 Media for freshwater, terrestrial, hot spring and salt water algae
Ca( NO3 )2 · 4H2O | 2.0 mg |
KH2PO4 | 0.62 mg |
MgSO4 · 7H2O | 2.5 mg |
Na2CO3 | 2 mg |
Na2SiO3 · 9H2O | 2.5 mg |
HCl ( 1 mol / L ) 1 ) | 0.025 mL |
Na2EDTA · 2H2O | 0.2 mg |
FeCl3 · 6H2O | 0.1 mg |
H3BO3 | 0.248 mg |
MnCl2 · 4H2O | 0.139 mg |
( NH4 )6Mo7O24 · 4H2O | 0.1 mg |
Vitamin B12 | 1 μg |
Thiamine HCl | 0.1 μg |
Biotin | 0.1 μg |
Distilled water | 100 mL |
1 ) In the NIES–Collection, pH is adjusted to 7.6 with 1 mol / L HCl.
Reference(s) |
---|
Chu, S. P. 1942 The influence of the mineral composition of the medium on the growth of planktonic algae. Part I. Methods and culture media. J. Ecol., 30, 284-325. |
1 Media for freshwater, terrestrial, hot spring and salt water algae
KNO3 | 100 mg |
MgSO4 · 7H2O | 25 mg |
K2HPO4 | 25 mg |
NaCl | 10 mg |
CaCl2 · 2H2O | 1 mg |
Fe solution 1 ) | 0.1 mL |
A5 solution 2 ) | 0.1 mL |
Agar | 1.5 g |
Distilled water | 99.8 mL |
pH 8.0 |
1 ) See Fe solution
2 ) See A5 solution
Reference(s) |
---|
Watanabe, A. 1960 List of algal strains in collection at the Institute of Applied Microbiology, University of Tokyo. J. Gen. Appl. Microbiol., 6, 283-292. |
1 Media for freshwater, terrestrial, hot spring and salt water algae
Ca ( NO3 )2·4H2O | 2 mg |
KNO3 | 10 mg |
β–Na2 glycerophosphate·5H2O | 3 mg |
MgSO4 · 7H2O | 2 mg |
Vitamin B12 | 0.01 µg |
Biotin | 0.01 µg |
Thiamine HCl | 1 µg |
PIV metals 1 ) | 0.1 mL |
Fe ( as EDTA; 1:1 molar ) 2 ) | 0.1 mL |
HEPES | 40 mg |
Distilled water | 99.8 mL |
pH 7.2 |
1 ) See PIV metals
2 ) See Fe ( as EDTA; 1:1 molar )
Reference(s) |
---|
Ichimura, T. 1973 The life cycle and its control in some species of Closterium, with special reference to the biological species problems. Thesis D. Sci., University of Tokyo, 69 pp., 11 tables, 40 figs. |
1 Media for freshwater, terrestrial, hot spring and salt water algae
MG 1 ) medium with
pH adjusted to 6.5 by buffering with MES instead of HEPES.
1 ) See MG
1 Media for freshwater, terrestrial, hot spring and salt water algae
To 100 mL AF–6 1 ) medium, add 40mg glucose,
yeast extract, tryptone, and sodium acetate.
1 ) See AF–6
Reference(s) |
---|
Nozaki, H., Watanabe, M. M. & Aizawa, K. 1995 Morphology and paedogamous sexual reproduction in Chlorogonium capillatum sp. nov. ( Volvocales, Chlorophyta ). J. Phycol., 31, 655-663. |
1 Media for freshwater, terrestrial, hot spring and salt water algae
1M HEPES–NaOH 1 ) | 2 mL |
K2HPO4 · 3H2O | 4 mg |
MgSO4 · 7H2O | 7.5 mg |
CaCl2 · 2H2O | 3.6 mg |
Citric acid | 0.6 mg |
Ferric ammonium citrate | 0.6 mg |
Na2EDTA – Mg | 0.1 mg |
Na2CO3 | 2 mg |
Trace metal mix A5 + Co 2 ) | 0.1 mL |
Vitamin B12 | 0.1 µg |
Biotin | 0.1 µg |
Thiamine HCl | 200µg |
Agar | 1.2 g |
Distilled water | 97.9 mL |
1 ) To 60 mL of Distilled water add 23.8 g HEPES, and pH is adjusted to
7.5. Bring to 100 mL with distilled water.v
2 ) See Trace metal mix A5 + Co
Reference(s) |
---|
Castenholz, R. W. 1988 Cultureing methods for cyanobacteria. Methods Enzymol., 167, 68-93. |
1 Media for freshwater, terrestrial, hot spring and salt water algae
NaCl | 3 mg |
MgCl2 · 6H2O | 150 mg |
KNO3 | 100 mg |
MgSO4 · 7H2O | 50 mg |
CaCl2 · 2H2O 1) | 26.5 mg |
KCl | 20 mg |
NaHCO3 | 4.3 mg |
KH2PO4 | 3.5 mg |
Tris ( hydroxymethyl ) aminomethane | 245 mg |
Distilled water | 100 mL |
pH 8.5 |
1 ) In the NIES-Collection, 20 mg CaCl2 is replaced by 26.5 mg CaCl2 · 2H2O.
Reference(s) |
---|
Johnson, M. K., Johnson, E. J., MacElroy, R. D., Speer, H. L., Bruff, B. S. 1968 Effects of salts on the halophilic alga Dunaliella viridis. J. Bacteriol., 95, 1461-1468. |
Utsunomiya, A., Watanuki, T., Matsushita, K., Tomita, I. 1997 Toxic effects of linear alkylbenzene sulfonate, quaternaryalkylammonium chloride and their complexes on Dunaliellasp. and Chlorella pyrenoidosa. Environ. Toxicol. Chem., 16, 1247-1254. |
Mihirogi, M., Kikuchi, M., Sawai, J. 2012 Development of screening algal growth inhibition test with Dunaliella sp. Jpn. J. Environ. Toxicol., 15, 11-16 (in Japanese with English summary). |
1 Media for freshwater, terrestrial, hot spring and salt water algae
CaCl2 · 2H2O | 0.5 mg |
NaNO3 | 2.5 mg |
NH4Cl | 0.5 mg |
CaSO4 · 2H2O | 0.4 mg |
MgSO4 · 7H2O | 0.5 mg |
Na2SiO3 · 9H2O | 0.2 mg |
Fe ( as EDTA; 1:1 molar ) 1 ) | 25 mL |
mM–1 Trace elements 2 ) | 0.1 mL |
K2HPO4 | 6.96 mg |
KH2PO4 | 266.5 mg |
Distilled water | 74.9 mL |
pH 5.1 – 5.3 |
1 ) See Fe ( as EDTA; 1:1 molar )
2 ) See mM–1 Trace elements
Reference(s) |
---|
Hoham, R. W., Berman, J. D., Rogers, H. S., Felio, J. H., Ryba, J. B., Miller, P. R. 2006 Two new species of green snow algae from Upstate New York, Chloromonas chenangoensis sp. nov. and Chloromonas tughillensis sp. nov. ( Volvocales, Chlorophyceae ) and the effects of light on their life cycle development. Phycologia, 45, 319-330. |
1 Media for freshwater, terrestrial, hot spring and salt water algae
Urea | 0.85 mg |
NaNO3 | 0.17 mg |
NH4Cl | 0.042 mg |
Ca( NO3 )2 · 4H2O | 10 mg |
CaCO3 | 1 mg |
CaCl2 · 2H2O | 1.4 mg |
KNO3 | 1 mg |
KHCO3 | 0.9 mg |
β–Na2glycerophosphate · 5H2O | 2 mg |
MgSO4 · 7H2O | 1.5 mg |
PIV metals 1 ) | 0.05 mL |
Vitamin B12 | 0.02 μg |
Thiamine HCl | 2 μg |
Biotin | 0.02 μg |
Glycylglycine | 10 mg |
Distilled water | 100 mL |
pH 7.2 |
1 ) See PIV metals
Reference(s) |
---|
Sako, Y., Ishida, Y., Kadota, H., Hata, Y. 1984 Sexual reproduction and cyst formation in the freshwater dinoflagellate Peridinium cunningtonii. Bull. Jpn. Soc. Sci. Fish., 50, 743-750. |
1 Media for freshwater, terrestrial, hot spring and salt water algae
MW 1 ) medium is diluted with distilled water
to 1/5.
1 ) See MW
1 Media for freshwater, terrestrial, hot spring and salt water algae
NaCl | 7 mg |
MgSO4 · 7H2O | 38 mg |
CaCl2 · 2H2O | 10.6 mg |
K2HPO4 | 60 mg |
Fe2( SO4 )3 · 6H2O | 1 mg |
Na2EDTA · 2H2O | 2.7 mg |
H3BO3 | 0.3 mg |
MnSO4 · 4H2O 1 ) | 0.2 mg |
Na2MoO4 · 2H2O | 0.8 mg |
ZnSO4 · 7H2O | 0.03 mg |
CuSO4 · 5H2O | 8 μg |
CoCl2 · 6H2O | 3.7 μg |
Agar | 1.5 g |
Distilled water | 100 mL |
pH 7.5 |
1 ) In the NIES–Colletion, 0.2 mg MnSO4 · 4H2O is replaced by 0.22 mg MnSO4 · 5H2O.
1 Media for freshwater, terrestrial, hot spring and salt water algae
Glucose | 100 mg |
Tryptone | 100 mg |
Yeast extract | 100 mg |
Beef extract 1 ) | 50 mg |
Agar | 150 mg |
Distilled water | 100 ml |
1 ) In the NIES-Collection, beef extract is removed.
Indicated as “Ochomonas medium” in reference.
Reference(s) |
---|
Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae ( I ) [17. Bisaisôrui no hozonhô ( I )]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 ( in Japanese without English title ). |
Starr, R. C. 1964 The culture collection of algae at Indiana University. Amer. J. Bot., 51, 1013-1044. |
1 Media for freshwater, terrestrial, hot spring and salt water algae
NH4NO3 | 10 mg |
MgSO4 · 7H2O | 4 mg |
KCl | 5 mg |
CaCl2 · 2H2O | 7.4 mg |
β–Na2glycerophosphate · 5H2O | 5 mg |
Sodium acetate | 100 mg |
Vitamin B12 | 0.01 μg |
Biotin | 0.01 μg |
Thiamine HCl | 1 μg |
PIV metals 1 ) | 0.3 mL |
Tris ( hydroxymethyl ) aminomethane | 50 mg |
Distilled water | 99.7 mL |
pH 8.0 |
1 ) See PIV metals
Reference(s) |
---|
Ichimura, T. 1979 2. Isolation and culture methods of algae. 2.5.B. Freshwater algae [2. Sôrui no bunri to baiyôhô. 2.5.B. Tansui sôrui]. In Methods in Phycological Studies [Sôrui Kenkyûhô ], Eds. by Nishizawa, K. & Chihara, M., Kyoritsu Shuppan, Tokyo, p. 294-305 ( in Japanese without English title ). |
1 Media for freshwater, terrestrial, hot spring and salt water algae
To 100 mL MBM 1 ) medium add 100 mg proteose
peptone.
1 ) See MBM
Indicated as “Proteose medium” in reference.
Reference(s) |
---|
Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae ( I ) [17. Bisaisôrui no hozonhô ( I )]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 ( in Japanese without English title ). |
Starr, R. C. 1964 The culture collection of algae at Indiana University. Amer. J. Bot., 51, 1013-1044. |
1 Media for freshwater, terrestrial, hot spring and salt water algae
NaHCO3 | 1.68 g |
K2HPO4 | 50 mg |
NaNO3 | 250 mg |
K2SO4 | 100 mg |
NaCl | 100 mg |
MgSO4 · 7H2O | 20 mg |
CaCl2 · 2H2O | 4 mg |
FeSO4 · 7H2O | 1 mg |
Na2EDTA · 2H2O | 8 mg |
A5 solution 1 ) | 0.1 mL |
Distilled water | 99.9 mL |
1 ) See A5 solution
Reference(s) |
---|
Ogawa, T., Terui, G. 1970 Studies on the growth of Spirulina platensis. ( I ) On the pure culture of Spirulina platensis. J. Ferment. Technol., 48, 361-367. |
1 Media for freshwater, terrestrial, hot spring and salt water algae
Put a small amount of dried soil into a test tube and add 20mL distilled water.
Reference(s) |
---|
Pringsheim, E. G. 1946 The biphasic or soil-water culture method for growing algae and flagellata. J. Ecol., 33, 193-204. |
1 Media for freshwater, terrestrial, hot spring and salt water algae
NH4Cl | 40 mg |
CaCl2 · 2H2O | 5.1 mg |
MgSO4 · 7H2O | 10 mg |
K2HPO4 | 11.9 mg |
KH2PO4 | 6.03 mg |
Hutner ’s trace elements 1 ) | 0.1 mL |
Acetic acid | 0.1 mL |
Tris ( hydroxymethyl ) aminomethane | 242 mg |
Agar | 1.5 g |
Distilled water | 99.8 mL |
1 ) See Hutner ’s trace elements
1 Media for freshwater, terrestrial, hot spring and salt water algae
To 100 mL MBM 1 ) medium add 1 g proteose
peptone and 2 g glucose.
1 ) See MBM
Indicated as “Trebouxia medium” in reference.
Reference(s) |
---|
Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae ( I ) [17. Bisaisôrui no hozonhô ( I )]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 ( in Japanese without English title ). |
Starr, R. C. 1964 The culture collection of algae at Indiana University. Amer. J. Bot., 51, 1013-1044. |
1 Media for freshwater, terrestrial, hot spring and salt water algae
NH4NO3 | 0.5 mg |
β–Na2glycerophosphate · 5H2O | 0.4 mg |
MgSO4 · 7H2O | 1 mg |
CaCl2 · 2H2O | 1 mg |
KCl | 0.1 mg |
Thiamine HCl | 1 μg |
Vitamin B12 | 0.01 μg |
Biotin | 0.01 μg |
Fe-EDTA | 0.05 mg |
PIV metals 1 ) | 0.1 mL |
Distilled water | 99.9 mL |
pH 7.5 2 ) |
1 ) See PIV metals
2 ) pH is adjusted to 7.5 with 0.1 mol / L HCl.
Reference(s) |
---|
Kimura, B., Ishida, Y. 1985 Photophagotrophy in Uroglena americana, Chrysophyceae. Jpn. J. Limnol., 46, 315-318. |
Nakahara, H., Sako, Y. 1987 2. Life history of freshwater phytoplankton [2. Tansui syokubutsu purankuton no seikatsushi]. In Freshwater red tide [Tansui Akashio ], Ed. by Kadota, H., Kôseisya-Kôseikaku, Tokyo, p. 21-77 ( in Japanese without English title ). |
1 Media for freshwater, terrestrial, hot spring and salt water algae
To 100 mL URO 1 ) medium add 40 mg HEPES.
1 ) See URO
1 Media for freshwater, terrestrial, hot spring and salt water algae
Prepare as for 100 mL URO 1 ) medium with
89.9 mL instead of 99.9 mL distilled water, add 10 mL Soil extract 2 ) and
adjust pH 7.5.
1 ) See URO
2 ) See Soil extract
1 Media for freshwater, terrestrial, hot spring and salt water algae
To 100 mL URO 1 ) medium add 50 mg Tris (
hydroxymethyl ) aminomethane.
1 ) See URO
1 Media for freshwater, terrestrial, hot spring and salt water algae
Ca( NO3 )2 · 4H2O | 11.78 mg |
β-Na2glycerophosphate · 5H2O | 5 mg |
MgSO4 · 7H2O | 4 mg |
KCl | 5 mg |
Vitamin B12 | 0.01 μg |
Biotin | 0.01 μg |
Thiamine HCl | 1 μg |
PIV metals 1 ) | 0.3 mL |
Glycylglycine | 50 mg |
Distilled water | 99.7 mL |
pH 7.5 |
1 ) See PIV metals
Reference(s) |
---|
Provasoli, L., Pintner, I. J. 1959 Artificial media for fresh-water algae: problems and suggestions. In The Ecology of Algae. Spec. Pub. No. 2,, Eds. by Tryon, C. A., Jr. & Hartmann, R. T., Pymatuning Laboratory of Field Biology, University of Pittsburgh, Pittsburgh, p. 84-96. |
Starr, R. C. 1973 Special methods-dry soil samples. In Handbook of Phycological Methods. Culture Methods and Growth Measurements, Ed. by Stein, J. R., Cambridge University Press, Cambridge, p. 159-167. |
1 Media for freshwater, terrestrial, hot spring and salt water algae
To 100 mL VT 1 ) medium add 20 mg sodium acetate.
1 ) See VT
Reference(s) |
---|
Nozaki, H., Kuroiwa, H., Mita, T., Kuroiwa, T. 1989 Pleodorina japonica sp. nov. ( Volvocales, Chlorophyta ) with bacteria-like endosymbionts. Phycologia, 28, 252-267. |
1 Media for freshwater, terrestrial, hot spring and salt water algae
To 100 mL VT 1 ) medium add 10 mg yeast extract
and 20 mg tryptone.
1 ) See VT
Reference(s) |
---|
Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae ( I ) [17. Bisaisôrui no hozonhô ( I ) ]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 ( in Japanese without English title ). |
1 Media for freshwater, terrestrial, hot spring and salt water algae
Ca( NO3 )2 · 4H2O | 10 mg |
KNO3 | 1 mg |
MgSO4 · 7H2O | 1.5 mg |
β–Na2glycerophosphate · 5H2O | 2 mg |
Urea | 1.7 mg |
Thiamine HCl | 0.2 μg |
Vitamin B12 | 0.002 μg |
Biotin | 0.002 μg |
PIV metals 1 ) | 0.05 mL |
Glycylglycine | 10 mg |
Distilled water | 99.95 mL |
pH 7.5 |
1 ) See PIV metals
Reference(s) |
---|
Watanabe, M. M. 1983 Growth characteristics of freshwater red tide alga, Peridinium based on axenic culture. Establishment of synthetic culture medium [Junsuibaiyôhô niyoru tansuiakashio Peridinium no zôsyokutokusei no kaiseki-gôseibaichi no kakuritsu ]. Res. Data Natl. Inst. Environ. Stud., No. 24, 111-121 ( in Japanese without English title ). |
1 Media for freshwater, terrestrial, hot spring and salt water algae
NaNO3 | 8.5 mg |
CaCl2 · 2H2O | 3.67 mg |
MgSO4 · 7H2O | 3.69 mg |
NaHCO3 | 1.26 mg |
Na2SiO3 · 9H2O | 2.84 mg |
K2HPO4 | 0.87 mg |
WC trace metals 1 ) | 0.1 mL |
Vitamin B12 | 0.05 μg |
Biotin | 0.05 μg |
Thiamine HCl | 10 μg |
Distilled water | 99.9 mL |
pH 7.5 |
1 ) See WC trace metals
Reference(s) |
---|
Guillard, R. R. L., Lorenzen, C. J. 1972 Yellow-green algae with chlorohyllide C. J. Phycol., 8, 10-14. |
Andersen, R. A. E, Berges, J. A., Harrison, P. J., Watanabe, M. M. 2005 Appendix A―Recipes for freshwater and seawater media. In Algal culturing techniques, Ed. by Andersen, R. A. E, Elsevier Academic Press, p. 474. |
1 Media for freshwater, terrestrial, hot spring and salt water algae
NaNO3 | 25 mg |
KH2PO4 | 17.5 mg |
K2HPO4 | 10 mg |
MgSO4 · 7H2O | 7.5 mg |
CaCl2 · 2H2O | 2.5 mg |
NaCl | 2.5 mg |
KOH | 3.1 mg |
Na2EDTA· 2H2O | 5 mg |
FeSO4 · 7H2O | 0.498 mg |
H3BO3 | 1.142 mg |
ZnSO4 · 7H2O | 0.882 mg |
MnCl2 · 4H2O | 0.144 mg |
MoO3 | 0.071 mg |
CuSO4 · 5H2O | 0.157 mg |
Co( NO3 )2 · 6H2O | 0.049 mg |
Vitamin B12 | 0.01 μg |
Biotin | 0.01 μg |
Thiamine HCl | 1 μg |
Soil extract 1 ) | 10 mL |
Distilled water | 100 mL |
Add 1.5 g agar to 100 mL of medium to give a solid medium.
1 ) See Soil extract
Reference(s) |
---|
Bischoff, H. W., Bold, H. C. 1963 Some soil algae from enchanted rock and related algal species. Phycological Studies IV.,Univ. No. 6318, Texas, p. 95. |
Watanabe, S., Fučíková, K., Lewis, L. A., Lewis P.O. 2016 Hiding in plain sight: Koshicola spirodelophila gen. et sp. nov. (Chaetopeltidales, Chlorophyceae), a novel green alga associated with the aquatic angiosperm Spirodela polyrhiza. Am. J. Bot., 103, 865-875. |
2 Media for marine and brackish water microalgae
Make diluted seawater by mixing 27.5 mL seawater and 70 mL distilled water.
Make ESM 1 ) medium by using this diluted seawater
instead of original seawater.
1 ) See ESM
2 Media for marine and brackish water microalgae
Make diluted seawater by mixing 47.5 mL seawater and 50 mL distilled water.
Make ESM 1 ) medium by using this diluted seawater
instead of original seawater.
1 ) See ESM
2 Media for marine and brackish water microalgae
NaNO3 | 12 mg |
K2HPO4 | 0.5 mg |
Vitamin B12 | 0.1 μg |
Biotin | 0.1 μg |
Thiamine HCl | 10 μg |
Fe – EDTA | 25.9 μg |
Mn – EDTA | 33.2 μg |
Tris ( hydroxymethyl ) aminomethane | 100 mg |
Soil extract 1 ) | 2.5 mL |
Seawater | 97.5 mL |
pH 8.0 |
Add 1.5 g agar to 100 mL of medium to give a solid medium.
1 ) The amount of Soil extract depends on the
quality of the soil. In the NIES–Collection, Soil extract was reduced from
5 mL to 2.5 mL after 2002. See Soil extract.
Reference(s) |
---|
Okaichi, T., Nishio, S., Imatomi, Y. 1982 Collection and mass culture [Shiryô no saisyu to baiyô]. In Toxic phytoplankton - Occurrence, mode of action, and toxins [Yûdoku Purankuton -Hassei, Sayôkikô, Dokuseibun ], Ed. by Jpn. Fish. Soc., Kôseisya-Kôseikaku, Tokyo, p. 22-34 ( in Japanese without English title ). |
2 Media for marine and brackish water microalgae
Mix 100 mL of ESM 1 ) medium and 0.25 mL of
CB–V 2 ) medium.
1 ) See ESM
2 ) See CB–V
2 Media for marine and brackish water microalgae
Prepare as for 100 mL ESM 1 ) medium with
95.5mL instead of 97.5 mL seawater and with 5 mL instead of 2.5 mL Soil
extract 2 ).
1 ) See ESM
2 ) See Soil extract
2 Media for marine and brackish water microalgae
NaNO3 | 7.5 mg |
NaH2PO4 · 2H2O | 0.6 mg |
Vitamin B12 | 0.05 μg |
Biotin | 0.05 μg |
Thiamine HCl | 10 μg |
Na2SiO3 · 9H2O | 1 mg |
f/2 metals 1 ) | 0.1 mL |
Seawater | 99.9 mL |
1 ) See f/2 metals
Reference(s) |
---|
Guillard, R. R. L., Ryther, J. H. 1962 Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt, and Detonula confervacea ( Cleve ) Gran. Can. J. Microbiol., 8, 229-239. |
2 Media for marine and brackish water microalgae
To 100 mL f/2 1 ) medium add 2.67 mg NH4Cl.
1 ) See f/2
2 Media for marine and brackish water microalgae
Into 100 mL seawater dissolve 25.2 mg powder medium of Daigo IMK ( Nihon
Pharmaceutical Co., Ltd. ). In the NIES–Collection, IMK medium is used after
autoclaving ( 121°C, 20 min ).
2 Media for marine and brackish water microalgae
Into 100 mL seawater dissolve 25.2 mg powder medium of Daigo IMK ( Nihon Pharmaceutical Co., Ltd. ). Adjust to salinity 10 percent with NaCl. In the NIES-Collection, IMK + NaCl medium is used after autoclaving ( 121°C, 20 min ).
2 Media for marine and brackish water microalgae
Into 100 mL seawater dissolve 25.2 mg powder medium of Daigo IMK ( Nihon
Pharmaceutical Co., Ltd. ). Thereafter, 2 mL Soil
extract 1 ) is added. In the NIES–Collection, IMK + Soil Extract medium
is used after autoclaving ( 121°C, 20 min ).
1 ) See Soil extract
2 Media for marine and brackish water microalgae
Into 100 mL seawater dissolve 12.6 mg powder medium of Daigo IMK ( Nihon
Pharmaceutical Co., Ltd. ). In the NIES–Collection, IMK/2 medium is used after
autoclaving ( 121°C, 20 min ).
Reference(s) |
---|
Takahashi, K., Lum, W. M., Benico, G., Uchida, H., Ozawa, M., Oikawa, H., Suzuki, T., Nguyen, N. V., Ha, D. V., Iwataki, M. 2021 Harmful algal bloomsToxigenic strains of Azadinium poporum (Amphidomataceae, Dinophyceae) from Japan and Vietnam, with first reports of A. poporum (ribotype A) and A. trinitatum in Asian Pacific. J. Phycol., 69, 175-187. |
2 Media for marine and brackish water microalgae
Tris-base ( pH 7.2) 1 ) | 0.1 mL |
NaNO3 | 7.5 mg |
NH4Cl | 0.267 mg |
β–Na2glycerophosphate · 5H2O | 0.216 mg |
H2SeO3 | 0.129 μg |
Vitamin B12 | 0.05 μg |
Biotin | 0.05 μg |
Thiamine HCl | 0.01 mg |
Na2SiO3 · 9H2O2 ) | 1.535 mg |
K metals 3 ) | 0.1 mL |
Seawater | 99.8 mL |
1 ) To 100 mL of Distilled water add 12.11 g Tris-base, and pH is adjusted
to 7.2.
2 ) In the NIES-Collection, Na2SiO3 · 9H2O
is removed.
3 ) See K metals
Reference(s) |
---|
Keller, M. D., Guillard, R.R.L. 1985 Factors significant to marine diatom culture. pp. 113-6. In Anderson, D.M., White, A.W., Baden, D.G. (eds.) Toxic Dinoflagellates. Elsevier, New York. |
Keller, M.D., Selvin, R.C., Claus, W., Guillard, R.R.L. 1987. Media for the culture of oceanic ultraphytoplankton. J. Phycol., 23: 633-638. |
2 Media for marine and brackish water microalgae
NaNO3 | 2.44 mg |
NH4Cl | 0.0267 mg |
KH2PO4 | 0.244 mg |
Fe-EDTA | 0.215 mg |
Vitamin B12 | 0.05 μg |
Biotin | 0.0005 μg |
Thiamine HCl | 0.01 mg |
K/2 metals 1 ) | 0.05 mL |
Seawater | 99.95 mL |
pH 8.2 |
K medium is diluted half strength and modified by Ian. This medium should
not be autoclaved but filter-sterilized.
1 ) See K/2 metals
Reference(s) |
---|
Keller, M. D., Guillard, R.R.L. 1985 Factors significant to marine diatom culture. pp. 113-6. In Anderson, D.M., White, A.W., Baden, D.G. (eds.) Toxic Dinoflagellates. Elsevier, New York. |
Keller, M.D., Selvin, R.C., Claus, W., Guillard, R.R.L. 1987. Media for the culture of oceanic ultraphytoplankton. J. Phycol., 23: 633-638. |
2 Media for marine and brackish water microalgae
NaNO3 | 2.44 mg |
NH4Cl | 0.0267 mg |
KH2PO4 | 0.244 mg |
Fe-EDTA | 0.215 mg |
Vitamin B12 | 0.05 μg |
Biotin | 0.0005 μg |
Thiamine HCl | 0.01 mg |
K/2 metals 1 ) | 0.05 mL |
Soil extract 2 ) | 1.5 mL |
Seawater | 98.45 mL |
pH 8.2 |
K medium is diluted half strength and modified by Ian. This medium should
not be autoclaved but filter-sterilized.
1 ) See K/2 metals
2 ) See Soil extract
Reference(s) |
---|
Keller, M. D., Guillard, R.R.L. 1985 Factors significant to marine diatom culture. pp. 113-6. In Anderson, D.M., White, A.W., Baden, D.G. (eds.) Toxic Dinoflagellates. Elsevier, New York. |
Keller, M.D., Selvin, R.C., Claus, W., Guillard, R.R.L. 1987. Media for the culture of oceanic ultraphytoplankton. J. Phycol., 23: 633-638. |
2 Media for marine and brackish water microalgae
NTA | 7 mg |
NaCl | 2.5 g |
MgSO4 · 7H2O | 900 mg |
KCl | 70 mg |
CaCl2 · 2H2O | 30 mg |
NaNO3 | 5 mg |
NaH2PO4 · 2H2O | 2 mg |
Vitamin B12 | 0.1 μg |
Vitamin mix S3 1 ) | 1 mL |
Na2SiO3 · 9H2O | 1 mg |
PN metals 2 ) | 3 mL |
Tris ( hydroxymethyl ) aminomethane | 100 mg |
Distilled water | 96 mL |
pH 8.0 |
1 ) See Vitamin mix S3
2 ) See PN metals
Reference(s) |
---|
Watanabe, M. M., Satake, K. N. ( Eds. ) 1991 NIES-Collection. List of Strains, Third Edition, 1991, Microalgae and Protozoa. Microbial Culture Collection, National Institute for Environmental Studies, Tsukuba, 163 pp.k.pdf |
2 Media for marine and brackish water microalgae
Into 100 mL distilled water dissolve 3.74 g powder medium of DifcoTM Marine broth 2216 (Becton, Dickinson and Company) and 25.2 mg powder medium of Daigo IMK (Nihon Pharmaceutical Co., Ltd.). In the NIES-Collection, MB medium is used after autoclaving (121°C, 20 min).
Reference(s) |
---|
Ishii, Y., Maruyama, S., Fujimura-Kamada, K., Kutsuna, N., Takahashi, S., Kawata, M., Minagawa, J. 2018 Isolation of uracil auxotroph mutants of coral symbiont alga for symbiosis studies. Sci Rep, 8, 3237. |
2 Media for marine and brackish water microalgae
f/2 1 ) medium with Na2SiO3
· 9H2O replaced by 1 mL Soil extract
2 ) and adjusted to pH 8.0 by buffering with 100 mg Tris ( hydroxymethyl
) aminomethane.
1 ) See f/2
2 ) See Soil extract
2 Media for marine and brackish water microalgae
KNO3 | 75 mg |
KH2PO4 | 2.5 mg |
MgSO4 · 7H2O | 2 mg |
Fe–citrate | 250 μg |
Agar | 1.5 g |
Seawater | 50 mL |
Distilled water | 50 mL |
Reference(s) |
---|
Watanabe, A. 1960 List of algal strains in collection at the Institute of Applied Micro-biology, University of Tokyo. J. Gen. Appl. Microbiol., 6, 283-292. |
2 Media for marine and brackish water microalgae
NaNO3 | 2 mg |
K2HPO4 | 0.1 mg |
Na2HPO4 · 12H2O | 0.028 mg |
Vitamin B12 | 0.015 μg |
Biotin | 0.015 μg |
Thiamine HCl | 2 μg |
CoSO4 · 7H2O | 0.12 μg |
ZnSO4 · 7H2O | 0.24 μg |
MnCl2 · 4H2O | 0.9 μg |
CuSO4 · 5H2O | 0.006 μg |
Na2SeO3 | 0.003 μg |
Na2MoO4 · 2H2O | 0.07 μg |
Na2EDTA · 2H2O | 0.37 μg |
Fe–EDTA | 2.6 μg |
Mn–EDTA | 3.3 μg |
Seawater | 100 mL |
Vitamins should be added at the end of the preparation. This medium should not be autoclaved but filter–sterilized.
Reference(s) |
---|
Noël, M.-H., Kawachi, M., Inouye, I. 2004 Induced dimorphic life cycle of a coccolithophorid, Calyptrosphaera sphaeroidea ( Prymnesiophyceae, Haptophyta ). J. Phycol., 40, 112-129. |
2 Media for marine and brackish water microalgae
KNO3 | 1.26 mg |
K2HPO4 | 0.22 mg |
Na2SiO3 1 ) | 0.61 mg |
mIMR trace metals 2 ) | 0.1 mL |
Thiamine | 20 μg |
Vitamin B12 | 1 μg |
Biotin | 0.1 μg |
Seawater | 100 mL |
1 ) In the NIES–Collection, Na2SiO3 is replaced by
Na2SiO3·9H2O.
2 ) See mIMR trace metals
Reference(s) |
---|
Paasche, E., Bruback, S., Skattebol, S., Young, J. R., Green, J. C 1996 Growth and calcification in the coccolithophorid Emiliania huxleyi ( Haptophyceae ) at low salinities. Phycologia, 35, 394-403. |
2 Media for marine and brackish water microalgae
NaNO3 | 17 mg |
NaH2PO4· 2H2O | 1.56 mg |
Na2SiO3· 9H2O | 5.68 mg |
Na2EDTA· 2H2O | 1.12 mg |
Fe-EDTA | 0.084 mg |
Na2SeO3 | 0.0346 μg |
P–1 metals | 1 mL |
Vitamin B12 | 0.2 μg |
Vitamin mix S3 | 1 mL |
Tris ( hydroxymethyl ) aminomethane | 50 mg |
Seawater | 98.8 mL |
pH 7.7 – 7.8 |
1 ) See P–1 metals
2 ) See Vitamin mix S3
Reference(s) |
---|
Imai, I. 2012 High growth rates of the red tide flagellate Chattonella marina (Raphidophyceae) observed in culture. Hokudai Suisan Ihou, 62, 71-74 ( in Japanese with English summary ). |
2 Media for marine and brackish water microalgae
NaH2PO4 · 2H2O | 0.779 mg |
NH4Cl | 4.28 mg |
PRO–99 trace metals 1 ) | 0.01 mL |
Seawater | 100 mL |
1 ) See PRO–99 trace metals
2 Media for marine and brackish water microalgae
Make diluted seawater by mixing 87.5 mL seawater and 10mL distilled water.
Make ESM 1 ) medium by using this diluted seawater
instead of seawater.
1 ) See ESM
3 Bacteria-free check media for freshwater algae
Appropriate medium | 100 mL |
Proteose peptone | 100 mg |
Reference(s) |
---|
Ichimura, T., Watanabe, M. M. 1977 An axenic clone of Microcystis aeruginosa Kutz. emend. Elenkin from Lake Kasumigaura. Bull. Jpn. Soc. Phycol., 25, 177-181. |
3 Bacteria-free check media for freshwater algae
Appropriate medium | 100 mL |
Yeast extract | 500 mg |
Reference(s) |
---|
Ichimura, T., Watanabe, M. M. 1977 An axenic clone of Microcystis aeruginosa Kutz. emend. Elenkin from Lake Kasumigaura. Bull. Jpn. Soc. Phycol., 25, 177-181. |
3 Bacteria-free check media for freshwater algae
Appropriate medium | 100 mL |
Peptone | 500 mg |
Beef extract | 300 mg |
Reference(s) |
---|
Ichimura, T., Watanabe, M. M. 1977 An axenic clone of Microcystis aeruginosa Kutz. emend. Elenkin from Lake Kasumigaura. Bull. Jpn. Soc. Phycol., 25, 177-181. |
3 Bacteria-free check media for freshwater algae
Appropriate medium | 100 mL |
Glucose | 100 mg |
Peptone | 100 mg |
Reference(s) |
---|
Ichimura, T., Watanabe, M. M. 1977 An axenic clone of Microcystis aeruginosa Kutz. emend. Elenkin from Lake Kasumigaura. Bull. Jpn. Soc. Phycol., 25, 177-181. |
3 Bacteria-free check media for freshwater algae
Appropriate medium | 100 mL |
Sodium acetate | 50 mg |
Glucose | 50 mg |
Tryptone | 50 mg |
Yeast extract | 30 mg |
Reference(s) |
---|
Ichimura, T., Watanabe, M. M. 1977 An axenic clone of Microcystis aeruginosa Kutz. emend. Elenkin from Lake Kasumigaura. Bull. Jpn. Soc. Phycol., 25, 177-181. |
3 Bacteria-free check media for freshwater algae
Appropriate medium | 100 mL |
Yeast extract | 100 mg |
Tryptone | 200 mg |
Reference(s) |
---|
Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae ( I ) [17. Bisaisôrui no hozonhô ( I ) ]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 ( in Japanese without English title ). |
4 Bacteria-free check media for marine algae
ASP 7 1 ) | 100 mL |
Trypticase | 50 mg |
Yeast extract | 5 mg |
1 ) In the NIES-Collection, ASP 7 is replaced by f/2 medium. See f/2.
Reference(s) |
---|
Yoshida, Y., Kawaguchi, K. 1983 Buoyancy and phototaxis of Chattonella antiqua ( Hada ) Ono. Bull. Plankton Soc. Jpn., 30, 11-19 ( in Japanese with English summary ). |
4 Bacteria-free check media for marine algae
NaCl | 1.8 g |
MgSO4 · 7H2O | 500 mg |
KCl | 60 mg |
NaNO3 | 100 mg |
CaCl2 · 2H2O | 36.7 mg |
K2HPO4 | 6 mg |
Sucrose | 400 mg |
PII metals 1 ) | 2 mL |
FeCl3 · 6H2O | 48 μg |
Thiamine HCl | 10 μg |
Biotin | 0.1 μg |
Vitamin B12 | 0.2 μg |
C–Source Mix II 2 ) | 1 mL |
Tris ( hydroxymethyl ) aminomethane | 100 mg |
Distilled water | 97 mL |
pH 8.0 |
1 ) See PII metals
2 ) See C–Source Mix II
4 Bacteria-free check media for marine algae
NaNO3 | 20 mg |
K2HPO4 | 1 mg |
Sodium glutamate | 50 mg |
Glucose | 20 mg |
Glycine | 10 mg |
D,L–Alanine | 10 mg |
Vitamin mix 8 1 ) | 0.1 mL |
Trypticase | 20 mg |
Yeast autolysate 2 ) | 20 mg |
Sucrose | 100 mg |
Soil extract 3 ) | 5 mL |
Seawater | 80 mL |
Distilled water | 15 mL |
pH 7.5 |
1 ) In the NIES–Collection, Vitamin mix 8 is replaced by
Vitamin mix S3. See Vitamin mix
S3 .
2 ) In the NIES–Collection, yeast autolysate is replaced by yeast extract.
3 ) See Soil extract
Reference(s) |
---|
Provasoli, L., McLaughlin, J. J. A., Droop, M. R. 1957 The development of artificial media for marine algae. Arch. Mikrobiol., 25, 392-428. |
5 Trace metals, vitamin mixtures and soil extracts
EDTA · 2Na | 700 mg |
FeCl3 · 6H2O | 400 mg |
Distilled water | 100 mL |
Sterilize by passing through a Millipore filter ( 0.22 μm ).
5 Trace metals, vitamin mixtures and soil extracts
H3BO3 | 285 mg |
MnCl2 · 4H2O | 180 mg |
ZnCl2 | 10.5 mg |
Na2MoO4 · 2H2O | 39 mg |
CoCl2 · 6H2O | 4 mg |
CuCl2 · 2H2O | 4.3 mg |
Distilled water | 100 mL |
5 Trace metals, vitamin mixtures and soil extracts
H3BO3 | 286 mg |
MnSO4 · 7H2O 1 ) | 250 mg |
ZnSO4 · 7H2O | 22.2 mg |
CuSO4 · 5H2O | 7.9 mg |
Na2MoO4 · 2H2O | 2.1 mg |
Distilled water | 100 mL |
1 ) In the NIES–Collection, 250 mg MnSO4 · 7H2O is replaced by 217 mg MnSO4 · 5H2O.
Reference(s) |
---|
Holm-Hansen, O., Gerloff, G. C., Skoog, F. 1954 Cobalt as an essential element for blue-green algae. Physiol. Planta., 7, 665-675. |
5 Trace metals, vitamin mixtures and soil extracts
Fe–EDTA | 30.16 g |
MnCl2 · 4H2O | 1.79 g |
H3BO3 | 2.86 g |
ZnSO4 · 7H2O | 220 mg |
CuSO4 · 5H2O | 79 mg |
( NH4 )6Mo7O24 · 4H2O | 130 mg |
NH4VO3 | 23 mg |
Distilled water | 100 mL |
In the NIES–Collection, Allen metals are used after dilution with distilled water to 1/1,000.
Reference(s) |
---|
Allen, M. B. 1959 Studies with Cyanidium caldarium, an anomalously pigmented chlorophyte. Arch. Mikrobiol., 32, 270-277. |
5 Trace metals, vitamin mixtures and soil extracts
Glycine | 100 mg |
D,L-Alanine | 100 mg |
L-Asparagine | 100 mg |
Sodium acetate · 3H2O 1 ) | 200 mg |
Glucose | 200 mg |
L-Glutamic acid | 200 mg |
Distilled water | 100 mL |
1 ) In the NIES–Collection, 200 mg sodium acetate · 3H2O is replaced by 120 mg sodium acetate, anhydrous.
5 Trace metals, vitamin mixtures and soil extracts
NTA | 0.2 g |
D trace mix 1 ) | 1 mL |
FeCl3 · 6H2O | 0.58 mg |
CaSO4 · 2H2O | 120 mg |
MgSO4 · 7H2O | 200 mg |
NaCl | 16 mg |
KNO3 | 200 mg |
NaNO3 | 1.4 g |
Na2HPO4 2 ) | 220 mg |
Distilled water | 99 mL |
1 ) See D trace mix
2 ) In the NIES-Collection, 220 mg Na2HPO4 is replaced
by 550 mg Na2HPO4· 12H2O.
Reference(s) |
---|
Castenholz, R. W. 1969 Thermophilic blue-green algae and the thermal environment. Bacteriol. Rev., 33, 476-504. |
5 Trace metals, vitamin mixtures and soil extracts
conc · H2SO4 | 0.05 mL |
MnSO4 · H2O 1 ) | 228 mg |
ZnSO4 · 7H2O | 50 mg |
H3BO3 | 50 mg |
CuSO4 · 5H2O | 2.5 mg |
Na2MoO4 · 2H2O | 2.5 mg |
CoCl2 · 6H2O | 4.5 mg |
Distilled water | 100 mL |
1 ) In the NIES–Collection, 228 mg MnSO4 · H2O is replaced by 349 mg MnSO4 · 5H2O.
Reference(s) |
---|
Castenholz, R. W. 1969 Thermophilic blue-green algae and the thermal environment. Bacteriol. Rev., 33, 476-504. |
5 Trace metals, vitamin mixtures and soil extracts
MnCl2 · 4H2O | 20 mg |
ZnSO4 · 7H2O | 4 mg |
CoCl2 · 6H2O | 0.8 mg |
Na2MoO4 · 6H2O 1 ) | 2 mg |
Na3VO4 · 10H2O 2 ) | 0.2 mg |
H2SeO3 | 0.2 mg |
Distilled water | 100 mL |
1 ) In the NIES–Collection, Na2MoO4 · 6H2O
is replaced by Na2MoO4 · 2H2O.
2 ) In the NIES-Collection, 0.2 mg Na3VO4 · 10H2O
is replaced by 0.1 mg Na3VO4.
5 Trace metals, vitamin mixtures and soil extracts
Fe( NH4 )2( SO4 )2 · 6H2O | 70.2 mg |
Na2EDTA · 2H2O | 66 mg |
Distilled water | 100 mL |
1 mL of this solution contains 0.1 mg Fe.
Reference(s) |
---|
Provasoli, L. 1966 Media and prospects for the cultivation of marine algae. In Cultures and Collections of Algae, Eds. by Watanabe, A. & Hattori, A., Proc. U.S.-Japan Conf., Hakone, Sept. 1966., Jpn. Soc. Plant Physiol., p. 63-75. |
5 Trace metals, vitamin mixtures and soil extracts
FeSO4 · 7H2O | 200 mg |
Distilled water | 100 mL |
Conc · H2SO4 | 0.026 mL 1 ) |
1 ) 2 drops/500 mL
Reference(s) |
---|
Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae ( I ) [17. Bisaisôrui no hozonhô ( I )]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 ( in Japanese without English title ). |
5 Trace metals, vitamin mixtures and soil extracts
Na2EDTA · 2H2O | 440 mg |
FeCl3 · 6H2O | 316 mg |
CoSO4 · 7H2O | 1.2 mg |
ZnSO4 · 7H2O | 2.1 mg |
MnCl2 · 4H2O | 18 mg |
CuSO4 · 5H2O | 0.7 mg |
Na2MoO4 · 2H2O | 0.7 mg |
Distilled water | 100 mL |
Reference(s) |
---|
Guillard, R. R. L., Ryther, J. H. 1962 Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt, and Detonula confervacea ( Cleve ) Gran. Can. J. Microbiol., 8, 229-239. |
5 Trace metals, vitamin mixtures and soil extracts
Na2EDTA · 2H2O | 5 g |
ZnSO4 · 7H2O | 2.2 g |
H3BO3 | 1.14 g |
MnCl2 · 4H2O | 506 mg |
FeSO4 · 7H2O | 499 mg |
CoCl2 · 6H2O | 161 mg |
CuSO4· 5H2O | 157 mg |
( NH4 )6Mo7O24 · 4H2O | 110 mg |
Distilled water | 100 mL |
Adjust the pH to 6.5-6.8 with KOH ( ~1.6 g ). Store the solution in a refrigerator ( 5°C ). The solution should have turned to violet color before use. This process takes a while and is necessary.
5 Trace metals, vitamin mixtures and soil extracts
Na2EDTA · 2H2O | 3.722 g |
Fe-EDTA | 0.493 g |
FeCl3 · 6H2O | 0.315 g |
MnCl2 · 4H2O | 17.8 mg |
ZnSO4 · 7H2O | 2.3 mg |
CoSO4 · 7H2O | 1.405 mg |
Na2MoO4 · 2H2O | 0.726 mg |
CuSO4 · 5H2O | 0.25 mg |
Distilled water | 100 mL |
Reference(s) |
---|
Keller, M. D., Guillard, R.R.L. 1985 Factors significant to marine diatom culture. pp. 113-6. In Anderson, D.M., White, A.W., Baden, D.G. (eds.) Toxic Dinoflagellates. Elsevier, New York. |
Keller, M.D., Selvin, R.C., Claus, W., Guillard, R.R.L. 1987. Media for the culture of oceanic ultraphytoplankton. J. Phycol., 23: 633-638. |
5 Trace metals, vitamin mixtures and soil extracts
1 ) In the NIES–Collection, 0.149 mg NiCl2 · 6H2O is replaced by 0.812 mg NiCl2.
Reference(s) |
---|
Guillard, R. R. L., Ryther, J. H. 1962 Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt, and Detonula confervacea ( Cleve ) Gran. Can. J. Microbiol., 8, 229-239. |
5 Trace metals, vitamin mixtures and soil extracts
FeCl3 · 6H2O | 100 mg |
MnSO4 · 4H2O | 85.5 mg |
ZnSO4 · 7H2O | 25 mg |
Na2MoO4 · 2H2O | 13 mg |
CoCl2 · 6H2O | 0.4 mg |
CuSO4 · 5H2O | 0.4 mg |
Na2SeO3 | 0.173 mg |
Na2EDTA 1 ) | 600 mg |
Distilled water | 100 mL |
1 ) In the NIES–Collection, Na2EDTA is replaced by Na2EDTA · 2H2O.
5 Trace metals, vitamin mixtures and soil extracts
CuSO4 · 5H2O | 10 mg |
MnCl2 · 4H2O | 10 mg |
Br ( 1mol / L solution ) | 0.01 mL |
ZnSO4 · 7H2O | 10 mg |
CoCl2 · 6H2O | 5 mg |
BaCl2 · 2H2O | 1 mg |
H3BO3 | 10 mg |
FeCl3 · 6H2O | 10 mg |
Na2MoO4 · 2H2O | 5 mg |
Distilled water | 100 mL |
Indicated as “Trace elements” in reference.
Reference(s) |
---|
Hoham, R. W., Berman, J. D., Rogers, H. S., Felio, J. H., Ryba, J. B., Miller, P. R. 2006 Two new species of green snow algae from Upstate New York, Chloromonas chenangoensis sp. nov. and Chloromonas tughillensis sp. nov. ( Volvocales, Chlorophyceae ) and the effects of light on their life cycle development. Phycologia, 45, 319-330. |
5 Trace metals, vitamin mixtures and soil extracts
K2HPO4 | 0.28 g |
KH2PO4 | 40 mg |
Casein Digest | 0.2 g |
Yeast extract | 0.1 g |
NaCl | 0.75 g |
Mucin, gastric | 0.2 g |
Distilled water | 97 mL |
Sterilize Pre–solution by autoclaving ( 121°C, 15 min ) , add aseptically 3 mL horse serum and 50 μL 10% Tween 80 dissolved in absolute ethanol ( both filter–sterilized ). Keep in a cool place.
5 Trace metals, vitamin mixtures and soil extracts
Na2EDTA · 2H2O | 100 mg |
H3BO3 | 114 mg |
FeCl3 · 6H2O | 4.9 mg |
MnSO4 · 4H2O | 16.4 mg |
ZnSO4 · 7H2O | 2.2 mg |
CoSO4 · 7H2O | 480 μg |
Distilled water | 100 mL |
Reference(s) |
---|
Provasoli, L. 1963 Growing marine seaweeds. In Proceedings of the Fourth International Seaweed Symposium, University of Tokyo Press, Tokyo, p. 9-17. |
5 Trace metals, vitamin mixtures and soil extracts
H3BO3 | 618.3 mg |
MnCl2 · 4H2O | 69.25 mg |
ZnCl2 | 5.45 mg |
CoCl2 · 6H2O | 238 μg |
Distilled water | 100 mL |
1 ) 1) In the NIES-Collection, CuCl2 · 2H2O is removed.
Reference(s) |
---|
Imai, I. 2012 High growth rates of the red tide flagellate Chattonella marina (Raphidophyceae) observed in culture. Hokudai Suisan Ihou, 62, 71-74 (in Japanese with English summary). |
5 Trace metals, vitamin mixtures and soil extracts
Na2EDTA · 2H2O | 100 mg |
FeCl3 · 6H2O | 19.6 mg |
MnCl2 · 4H2O | 3.6 mg |
ZnCl2 1 ) | 1.04 mg |
CoCl2 · 6H2O | 0.4 mg |
Na2MoO4 · 2H2O | 0.25 mg |
Distilled water | 100 mL |
1 ) In the NIES–Collection, 1.04mg ZnCl2 is replaced by 2.2mg ZnSO4 · 7H2O.
Reference(s) |
---|
Provasoli, L., Pintner, I. J. 1959 Artificial media for fresh-water algae: problems and suggestions. In The Ecology of Algae. Spec. Pub. No. 2,, Eds. by Tryon, C. A., Jr. & Hartmann, R. T., Pymatuning Laboratory of Field Biology, University of Pittsburgh, Pittsburgh, p. 84-96. |
5 Trace metals, vitamin mixtures and soil extracts
Na2EDTA · 2H2O | 100 mg |
H3BO3 | 113 mg |
FeCl3 · 6H2O | 6.3 mg |
CoSO4 · 7H2O | 0.093 mg |
ZnSO4 · 7H2O | 4.66 mg |
MnCl2 · 4H2O | 3.2 mg |
Distilled water | 100 mL |
Reference(s) |
---|
Watanabe, M. M., Satake, K. N. ( Eds. ) 1991 NIES-Collection. List of Strains, Third Edition, 1991, Microalgae and Protozoa. Microbial Culture Collection, National Institute for Environmental Studies, Tsukuba, 163 pp. |
5 Trace metals, vitamin mixtures and soil extracts
Na2EDTA 1 ) | 0.58 g |
FeCl3 · 6H2O | 0.422 g |
ZnSO4 · 7H2O | 2.933 mg |
CoCl2 · 6H2O | 1.332 mg |
MnCl2 · 4H2O | 24 mg |
Na2MoO4 · 2H2O | 0.839 mg |
Na2SeO3 | 2.306 mg |
NiCl2 · 6H2O | 0.37 mg |
Distilled water | 100 mL |
1 ) In the NIES–Collection, 0.58 g Na2EDTA is replaced by 0.637 g Na2EDTA · 2H2O.
5 Trace metals, vitamin mixtures and soil extracts
To 1000 mL distilled water add 200 mL of soil ( soil from undisturbed deciduous woodland is best ) and heat by autoclaving for 1 h at 105°C. When cool, heat by autoclaving for 1 h at 105°C again. Pass the supernatant through a GF/C filter and Celite, and then pass the filtrate through a GF/F filter. Adjust to 1000 mL by adding distilled water. Dispense 10 mL of the final filtrate into each test tube and sterilize by autoclaving for 20min at 121°C. Keep in a cool place.
Reference(s) |
---|
Provasoli, L., McLaughlin, J. J. A., Droop, M. R. 1957 The development of artificial media for marine algae. Arch. Mikrobiol., 25, 392-428. |
5 Trace metals, vitamin mixtures and soil extracts
H3BO3 | 286 mg |
MnCl2 · 4H2O | 181 mg |
ZnSO4 · 7H2O | 22.2 mg |
Na2MoO4 · 2H2O | 39 mg |
CuSO4 · 5H2O | 7.9 mg |
Co( NO3 )2·6H2O | 4.9 mg |
Distilled water | 100 mL |
5 Trace metals, vitamin mixtures and soil extracts
Thiamine HCl | 5 mg |
Nicotinic acid | 1 mg |
Calcium pantothenate | 1 mg |
p–Aminobenzoic acid | 0.1 mg |
Biotin | 0.01 mg |
Inositol | 50 mg |
Folic acid | 0.02 mg |
Thymine | 30 mg |
Distilled water | 100 mL |
Reference(s) |
---|
Provasoli, L. 1963 Growing marine seaweeds. In Proceedings of the Fourth International Seaweed Symposium, University of Tokyo Press, Tokyo, p. 9-17. |
5 Trace metals, vitamin mixtures and soil extracts
EDTA | 0.522 g |
FeSO4 · 7H2O | 0.498 g |
KOH (1 mol/L solution) | 5.4 mL |
Distilled water | 94.6 mL |
EDTA and FeSO4 · 7H2O is heated for 30 min (100°C
); KOH is added to the cooled mixture.
Reference(s) |
---|
McFadden, G. I., Melkonian, M. 1986 Use of Hepes buffer for microalgael culture media and fixation for eledtron microscopy. Phycology, 25, 551-557. |
5 Trace metals, vitamin mixtures and soil extracts
Na2EDTA· 2H2O | 0.3 g |
H3BO3 | 0.114 g |
MnCl2· 4H2O | 14.4 mg |
ZnSO4·7H2O | 2.1 mg |
CoCl2·6H2O | 0.4 mg |
Distilled water | 100 mL |
Reference(s) |
---|
McFadden, G. I., Melkonian, M. 1986 Use of Hepes buffer for microalgael culture media and fixation for eledtron microscopy. Phycology, 25, 551-557. |
5 Trace metals, vitamin mixtures and soil extracts
Na2EDTA· 2H2O | 436 mg |
FeCl3 · 6H2O | 315 mg |
CuSO4 · 5H2O | 1 mg |
ZnSO4 · 7H2O | 2.2 mg |
CoCl2 · 6H2O | 1 mg |
MnCl2 · 4H2O | 18 mg |
Na2MoO4 · 2H2O | 0.6 mg |
H3BO3 | 100 mg |
Distilled water | 100 mL |
Reference(s) |
---|
Guillard, R. R. L., Lorenzen, C. J. 1972 Yellow-green algae with chlorohyllide C. J. Phycol., 8, 10-14. |
Andersen, R. A. E, Berges, J. A., Harrison, P. J., Watanabe, M. M. 2005 Appendix A — Recipes for freshwater and seawater media. In Algal culturing techniques, Ed. by Andersen, R. A. E, Elsevier Academic Press, p. 474. |
6 Media for protozoa
Beforehand, sterilize wheat grains by dry heating ( 150ºC, 30 min ). Keep
in a cool place. For use, add a grain of sterile wheat to 10 mL AF–6
1 ) medium.
1 ) See AF–6
6 Media for protozoa
BL solution: 1 pack ( 3 g ) of Barley leaf ( Trade name: Oomugi Wakaba Funmatu 100%, Yamamoto Kanpou Seiyaku Co., Ltd. ) is added to 1,000 mL distilled water and boiled for 10 minutes, while stirring. The solution is passed through the qualitative filter paper.
EY solution: 9 g of crushed yolk of hard-boiled egg is added to 1,000 mL distilled water and boiled for 10 minutes, while stirring. The solution is passed through the qualitative filter paper.
BL and EY Solution are mixed. The mixed solution is dilution with distilled water up to 6,000 mL, and then sterilized by autoclaving ( 121ºC, 15 min ).
6 Media for protozoa
Glucose | 0.2 g |
Tryptone | 0.1 g |
Yeast extract | 0.05 g |
Seawater | 100 mL |
6 Media for protozoa
Beforehand, sterilize polished rice by dry heating ( 150ºC, 30 min ). Keep
in a cool place. For use, add 500 μL mTYGM–9 1
) and a grain of sterile rice to 10 mL ESM 2
) medium.
1 ) See mTYGM–9
2 ) See ESM
6 Media for protozoa
Beforehand, sterilize polished rice by dry heating ( 150ºC, 30 min ). Keep
in a cool place. For use, add a grain of sterile rice to 10 mL ESM
1 ) medium.
1 ) See ESM
6 Media for protozoa
Beforehand, sterilize polished rice by dry heating ( 150ºC, 30 min ). Keep
in a cool place. For use, add 500 μL mTYGM–9 1
) and a grain of sterile rice to 10 mL f/2 2
) medium.
1 ) See mTYGM–9
2 ) See f/2
6 Media for protozoa
Beforehand, sterilize polished rice by dry heating ( 150ºC, 30 min ). Keep
in a cool place. For use, add a grain of sterile wheat to 10 mL f/2
1 ) medium.
1 ) See f/2
6 Media for protozoa
Beforehand, sterilize wheat grains by dry heating ( 150ºC, 30 min ). Keep
in a cool place. For use, add a grain of sterile wheat to 10 mL f/2
1 ) medium.
1 ) See f/2
6 Media for protozoa
Sodium acetate | 10 mg |
Polypeptone | 10 mg |
Tryptone | 20 mg |
Yeast extract | 20 mg |
CaCl2 · 2H2O | 0.1 mg |
Sea water | 10 mL |
Distilled water | 90 mL |
6 Media for protozoa
Into 98.9 mL distilled water 3.6 g powder medium of Daigo's Artificial Seawater SP ( Nihon Pharmaceutical Co., Ltd. ) and 0.1 mL LB broth medium ( BD ). After autoclaving, add aseptically 1 mL horse serum ( Sigma-Aldrich Co. LLC ).
Reference(s) |
---|
Tashyreva, D., Prokopchuk, G., Yabuki, A., Kaur, B., Faktorová, D., Votýpka, J., Kusaka, C., Fujikura, K., Shiratori, T., Ishida, K., Horák, A., Lukeš, J. 2018 Phylogeny and morphology of new diplonemids from Japan. Protist, 169, 158-179. |
6 Media for protozoa
L solution: White part of lettuce is dried at 90ºC for 16–18 h without
scorching; 300 mg of the dried lettuce is added to 100 mL boiling water (
9:1 distilled water to tap water ) and boiled for 30 min, while stirring.
The supernatant is passed through cotton wool.
E solution: 300 mg of crushed yolk of hardboiled egg is added to 100
mL water ( 9:1 distilled water to tap water ) and boiled for 30 min, while
stirring. The supernatant is passed through cotton wool.
Equal quantities of L and E solutions are mixed. The pH is adjusted to 6.8–7.0
with 1 mol / L NaOH, and 100 mL of the solution is dispensed into each 200–mL
Erlenmeyer flask and sterilized by autoclaving ( 121ºC, 15 min ).
6 Media for protozoa
Put 10 - 20 of dried water fleas commercially available for aquarium fish into a test tube and add 10mL distilled water.
Reference(s) |
---|
Takahashi, Y., Yoshida, M., Inouye, I., Watanabe, M. M. 2014 Diplophrys mutabilis sp. nov., a new member of Labyrinthulomycetes from freshwater habitats. Protist, 165, 50-65. |
6 Media for protozoa
HEPES | 11.915 mg |
KNO3 | 5 mg |
MgSO4 · 7H2O | 1 mg |
(NH4)2HPO4 | 1 mg |
Ca(NO3)2 · 4H2O | 5 mg |
Waris P-2 | 0.05 mL |
Waris EDTA | 0.05 mL |
Vitamin B12 | 0.01 μg |
Biotin | 0.05 μg |
Thiamine HCl | 5 μg |
Niacinamide | 0.005 μg |
Soil extract 3 ) | 0.5 mL |
Na2SiO3 · 9H2O | 2.842 mg |
Distilled water | 99.4 mL |
pH 7.0 | |
Waris H is diluted half strength and added Na2SiO3
· 9H2O.
1 ) See Waris P-2
2 ) See Waris EDTA
3 ) See Soil extract
Reference(s) |
---|
McFadden, G. I., Melkonian, M. 1986 Use of Hepes buffer for microalgael culture media and fixation for eledtron microscopy. Phycology, 25, 551-557. |
6 Media for protozoa
Proteose Peptone | 0.15 g |
Yeast extract | 0.1 g |
Glucose | 0.5 g |
Distilled water | 100 mL |
pH 7.0 |
PPYG medium is diluted half strength and reduced proteose peptone.
Reference(s) |
---|
Mori, K., Yomo, T., Kashiwagi, A. 2011 Single-cell isolation and cloning of tetrahymena thermophila cells with a fluorescence-activated cell sorter. J. Eukaryotic Microbiol., 58(1), 37-42. |
6 Media for protozoa
Prepare as for 100 mL URO 1 ) medium with
seawater instead of distilled water. Add 10 mg yeast extract and 20 mg tryptone.
Indicated as " URO–YT " in reference.
1 ) See URO
Reference(s) |
---|
Moriya, M., Nakayama, T., Inouye, I. 2000 Ultrastructure and 18S rDNA sequence analysis of Wobblia lunata gen. et sp. nov., a new heterotrophic flagellate ( Stramenopiles, incertae sedis ). Protist, 151, 41-55. |
6 Media for protozoa
Prepare as for 100 mL URO 1 ) medium with
seawater instead of distilled water. Add 1 mg yeast extract and 2 mg tryptone.
Indicated as " URO–1/10 YT " in reference.
1 ) See URO
Reference(s) |
---|
Moriya, M., Nakayama, T., Inouye, I. 2002 A new class of the Stramenopiles, Placididea Classis nova: description of Placidia cafeteriopsis gen. et sp. nov. Protist, 153, 143-156. |
6 Media for protozoa
Beforehand, sterilize polished rice by dry heating ( 150ºC, 30 min ). Keep
in a cool place. For use, add 500 μL mTYGM–9 1
) and a grain of sterile rice to 10 mL SUY 1/10
2 ) medium.
1 ) See mTYGM–9
2 ) See SUY 1/10
6 Media for protozoa
Beforehand, sterilize wheat grains by dry heating ( 150ºC, 30 min ). Keep
in a cool place. For use, add a grain of sterile wheat to 10 mL SUY
1/10 1 ) medium.
1 ) See SUY 1/10
6 Media for protozoa
Beforehand, sterilize wheat grains by dry heating ( 150ºC, 30 min ). Keep
in a cool place. For use, add a grain of sterile wheat to 10 mL URO
1 ) medium.
1 ) See URO
6 Media for protozoa
Beforehand, sterilize wheat grains by dry heating ( 150ºC, 30 min ). Keep
in a cool place. For use, add a grain of sterile wheat to 10 mL URO–H
1 ) medium.
1 ) See URO–H
6 Media for protozoa
Prepare as for 100 mL URO 1 ) medium. Adjust to pH 7.5 with 0.1 mol / L HCl, and add 10 mg yeast extract and 20 mg tryptone (URO + YT (1/10) medium).
Beforehand, sterilize wheat grains by dry heating ( 150ºC, 30 min ). Keep in a cool place. For use, add a grain of sterile wheat to 10 mL URO + YT (1/10) medium.
1 ) See URO
Reference(s) |
---|
Yabuki, A., Nakayama, T., Yubuki, N., Hashimoto, T., Ishida, K-I. Inagaki, Y. 2011 Tsukubamonas globosa n. gen., n. sp., a novel excavate flagellate possibly holding a key for the early evolution in "Discoba". J. Eukaryot. Microbiol., 58, 319-331. |
6 Media for protozoa
Prepare as for 100 mL URO 1 ) medium with
99.2 mL instead of 99.9 mL distilled water. Adjust to pH 7.5 with 0.1 mol
/ L HCl, and add 10 mg yeast extract, 20 mg tryptone and 0.3 ml horse serum
( UYTS medium ). Beforehand, sterilize polished rice by dry heating ( 150ºC,
30 min ). Keep in a cool place. For use, add a grain of sterile rice to 10
mL UYTS medium.
1 ) See URO
6 Media for protozoa
Into 100 mL distilled water 9 mg powder medium of Daigo's Artificial Seawater SP
( Nihon Pharmaceutical Co., Ltd. ) and 10 mg yeast extract.
7 Media for freshwater red algae
NaNO3 | 75 mg |
CaCl2 · 2H2O | 2.5 mg |
MgSO4 · 7H2O | 7.5 mg |
K2HPO4 | 7.5 mg |
KH2PO4 1 ) | 17.5 mg |
NaCl | 2.5 mg |
Vitamin B12 2 ) | 0. 015 μg |
PIV metals 3 ) | 0.6 mL |
Soil extract 4 ) | 4 mL |
Distilled water | 96.4 mL |
1 ) In the NIES–Collection, the amount of KH2PO4 is
reduced from 17.5 mg to 10.5 mg.
2 ) In the NIES–Collection, the amount of Vitamin B12 is increased
from 0.015 μg to 0.02 μg.
3 ) See PIV metals
4 ) See Soil extract
8 Media for Charales
Put leaf mould into a glass vessel to make a thin bottom layer, and add
river sand onto the bottom layer up to one–quarter to one–fifth from the bottom.
Add a pinch of garden lime to the river sand before use. Dampen the soil with
deionized water ( or distilled water ). Cover the glass vessel with a plastic
cap or aluminum foil, and autoclave it twice with overnight rest in between
( 121ºC, 20 min ). After cooling the mixture to room temperature, pour sterilized
deionized water ( or sterilized distilled water ) into it carefully ( so as
not to disturb the soil ). In the case of brackish water strains, deionized
water is replaced by about one–third–diluted Herbst artificial seawater (
1/3 Herbst ASW ).
Reference(s) |
---|
Sakayama, H., Hara Y., Nozaki, H. 2004 Taxonomic re-examination of six species of Nitella ( Charales, Charophyceae ) from Asia, and phylogenetic relationships within the genus based on rbcL and atpB gene sequences. Phycologia, 43, 91-104. |
8 Media for Charales
Put leaf mould into a glass vessel to make a thin bottom layer, and add black soil onto the bottom layer up to one-quarter to one–fifth from the bottom. Dampen the soil with deionized water ( or distilled water ). Cover the glass vessel with a plastic cap or aluminum foil, and autoclave it twice with overnight rest in between ( 121ºC, 20 min ). After cooling the mixture to room temperature, pour sterilized deionized water ( or sterilized distilled water ) into it carefully ( so as not to disturb the soil ).
Reference(s) |
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Sakayama, H., Hara Y., Nozaki, H. 2004 Taxonomic re-examination of six species of Nitella ( Charales, Charophyceae ) from Asia, and phylogenetic relationships within the genus based on rbcL and atpB gene sequences. Phycologia, 43, 91-104. |
8 Media for Charales
Put bottom mud from a paddy field into a glass vessel up to one–quarter
to one–fifth from the bottom. Dampen the mud with deionized water ( or distilled
water ). Cover the glass vessel or jar with a plastic cap or aluminum foil,
and autoclave it twice with overnight rest in between ( 121ºC, 20 min ). After
cooling the mixture to room temperature, pour sterilized deionized water (
or sterilized distilled water ) into it carefully ( so as not to disturb the
soil ).
8 Media for Charales
Put leaf mould into a glass vessel to make a thin bottom layer, and add a mixture of black soil and river sand onto the bottom layer up to one–quarter to one–fifth from the bottom. Dampen the soil with deionized water ( or distilled water ). Cover the glass vessel or jar with a plastic cap or aluminum foil, and autoclave it twice with overnight rest in between ( 121ºC, 20 min ). After cooling the mixture to room temperature, pour sterilized deionized water ( or sterilized distilled water ) into it carefully ( so as not to disturb the soil ). In the case of brackish water strains, deionized water is replaced by about one–third–diluted Herbst artificial seawater ( 1/3 Herbst ASW ).
8 Media for Charales
Put a mixture of black soil and bottom mud from a paddy field into a glass vessel up to one–quarter to one–fifth from the bottom. Dampen the soil with deionized water ( or distilled water ). Cover the glass vessel or jar with a plastic cap or aluminum foil, and autoclave it twice with overnight rest in between ( 121ºC, 20 min ). After cooling the mixture to room temperature, pour sterilized deionized water ( or sterilized distilled water ) into it carefully ( so as not to disturb the soil ).
8 Media for Charales
Put a mixture of river sand and bottom mud from a paddy field into a glass vessel up to one–quarter to one–fifth from the bottom. Dampen the soil with deionized water ( or distilled water ). Cover the glass vessel or jar with a plastic cap or aluminum foil, and autoclave it twice with overnight rest in between ( 121ºC, 20 min ). After cooling the mixture to room temperature, pour sterilized deionized water ( or sterilized distilled water ) into it carefully ( so as not to disturb the soil ).
8 Media for Charales
NaCl | 3.0 mg |
KCl 1 ) | 81.4 mg |
CaCl2 1 ) | 132 mg |
MgSO4 1 ) | 660 mg |
NaHCO3 1 ) | 504 mg |
Distilled water | 100 mL |
1 ) In the NIES–Collection, the amount of KCl is reduced from 81.4 mg to 80.0 mg, 132 mg CaCl2 is replaced by 172 mg CaCl2 · 2H2O, 660 mg MgSO4 is replaced by 1.35 g MgSO4 · 7H2O, and the amount of NaHCO3 is reduced from 504 mg to 49.5 mg.
Reference(s) |
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Okazaki, Y., Shimmen, T., Tazawa, M. 1984 Turgor regulation in a brackish charophyte, Lamprothamnium succinctum I. Artificial modification of intracellular osmotic pressure. Plant Cell Physiol., 25, 565-571. |