The Microbial Culture Collection at the National Institute for Environmental Studies

Strain Data


Strain number NIES-83  
Phylum Heterokontophyta   Class Raphidophyceae 
Scientific name Chattonella marina (Subrahmanyan) Y.Hara et Chihara var. antiqua (Hada) Demura et Kawachi  
Synonym -----  
Former name Chattonella antiqua (Hada) Ono  
Common name  
Locality (Date of collection) off Hiketa Kagawa Japan (1977-08-**)  
Habitat (Isolation source) Marine  
History < KAGAWA  
Isolator (Date of isolation) Ono, Chitaru (-----)  
Identified by Ono, Chitaru  
State of strain Unialgal; Clonal; Axenic[2013 Jan]  
Preservation state
(State of preservation)
Subculture  
Culture condition
( Preculture condition )
Medium:  f/2  
Temperature: 20 C
Light intensity: 32-40 µmol photons/m2/sec     L/D cycle: 12L:12D
Duration: 1M  
Gene information ITS1-5.8S-ITS2 ( AB334309 ) , CO1 ( AB334446 ) , rbcL ( AB334373 )
Cell size (min - max)   Organization Unicellular; Flagellate 
Characteristics Red tide  
Other strain no. Other strain no. : KGW-2  
Remarks Fragile species to transportation stresses 

Reference
78)   Demura, M., Noël, M.-H., Kasai, F., Watanabe, M. M., Kawachi, M. 2009 Taxonomic revision of Chattonella antiqua, C. marina and C. ovata (Raphidophyceae) based on their morphological characteristics and genetic diversity. Phycologia, 48, 518-535. , Keywords: Chattonella antiqua; Chattonella marina; Chattonella ovata; COI; COX1; Genetic diversity; ITS; Microsatellite; Morphology; Phylogeny; Population; Raphidophyceae; rbcL; Speciation; Taxonomy , Strain(s): 12356910148384858611311411511611812114514616129355755855956160367171684918721873 

226)   Hiroishi, S., Uchida, A., Nagasaki, K., Ishida, Y. 1988 A new method for identification of inter- and intra-species of the red tide algae Chattonella antiqua and Chattonella marina (Raphidophyceae) by means of monoclonal antibodies. J. Phycol., 24, 442-444. , Keywords: Chattonella; identification; immunofluorescence; inter-species; intra-species; method; monoclonal antibody; red tide , Strain(s): 121483848586113114115116118121 

1232)   Uchida, A., Nagasaki, K., Hiroishi, S., Ishida, Y. 1989 The application of monoclonal antibodies to an identification of Chattonella marina and Chattonella antiqua. Bull. Jpn. Soc. Sci. Fish., 55, 721-725. , Strain(s): 38386118121