The Microbial Culture Collection at the National Institute for Environmental Studies

Strain Data


Strain number NIES-2  
Phylum Heterokontophyta   Class Raphidophyceae 
Scientific name Chattonella marina (Subrahmanyan) Y.Hara et Chihara var. antiqua (Hada) Demura et Kawachi  
Synonym -----  
Former name Chattonella antiqua (Hada) Ono  
Common name  
Locality (Date of collection) Osaka Bay Osaka Japan (1982-09-**)  
Habitat (Isolation source) Marine  
History < Watanabe, Makoto M.  
Isolator (Date of isolation) Yamochi, Susumu (-----)  
Identified by Yamochi, Susumu  
State of strain Unialgal; Clonal; Non-axenic[2013 Feb]  
Preservation state
(State of preservation)
Subculture  
Culture condition
( Preculture condition )
Medium:  f/2ESM  
Temperature: 20 C
Light intensity: 32-40 µmol photons/m2/sec     L/D cycle: 12L:12D
Duration: 1M  
Gene information ITS1-5.8S-ITS2 ( AB334308 ) , CO1 ( AB334445 ) , rbcL ( AB334372 )
Cell size (min - max) 50 - 200 μm   Organization Unicellular; Flagellate 
Characteristics Red tide  
Other strain no. Other strain no. : OCH-a  
Remarks Fragile species to transportation stresses 

Reference
78)   Demura, M., Kawachi, M., Kunugi, M., Nishizawa, T., Kasai, F., Watanabe, M. M. 2007 Development of microsatellite markers for the red tide-forming harmful species Chattonella antiqua, C. marina, and C. ovata (Raphidophyceae). Mol. Ecol. Notes, 7, 315-317. , Keywords: Chattonella; harmful algal bloom; microsatellite; phytoplankton; Raphidophyceae; red tide , Strain(s): 2 

79)   Demura, M., Noël, M.-H., Kasai, F., Watanabe, M. M., Kawachi, M. 2009 Taxonomic revision of Chattonella antiqua, C. marina and C. ovata (Raphidophyceae) based on their morphological characteristics and genetic diversity. Phycologia, 48, 518-535. , Keywords: Chattonella antiqua; Chattonella marina; Chattonella ovata; COI; COX1; Genetic diversity; ITS; Microsatellite; Morphology; Phylogeny; Population; Raphidophyceae; rbcL; Speciation; Taxonomy , Strain(s): 12356910148384858611311411511611812114514616129355755855956160367171684918721873 

226)   Hiroishi, S., Uchida, A., Nagasaki, K., Ishida, Y. 1988 A new method for identification of inter- and intra-species of the red tide algae Chattonella antiqua and Chattonella marina (Raphidophyceae) by means of monoclonal antibodies. J. Phycol., 24, 442-444. , Keywords: Chattonella; identification; immunofluorescence; inter-species; intra-species; method; monoclonal antibody; red tide , Strain(s): 121483848586113114115116118121