Strain number NIES-2  
Phylum Heterokontophyta  
Class Raphidophyceae  
Scientific name Chattonella marina (Subrahmanyan) Y.Hara & Chihara var. antiqua (Hada) Demura & Kawachi  
Synonym Hemieutreptia antiqua Hada; Chattonella antiqua (Hada) Ono  
Former name Chattonella antiqua (Hada) Ono  
Common name  
Locality (Date of collection) Osaka Bay Osaka Japan (1982-09-**)  
Latitude / Longitude  
Habitat (Isolation source) Marine  
History < Watanabe, Makoto M.  
Isolator (Date of isolation) Yamochi, Susumu  
Identified by Yamochi, Susumu  
State of strain Subculture; Unialgal; Clonal; Non-axenic[2013 Feb]  
Culture condition
(Preculture condition)
Medium:  f/2ESM  
Temperature:  20 C
Light intensity:  21 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S-ITS1-5.8S-ITS2-28S ( AB286908 ) , ITS1-5.8S-ITS2 ( AB334308 ) , CO1 ( AB334445 AB445405 ) , CO2, CO1 ( AB286900 ) , rbcL ( AB334372 )  
Cell size (min - max) 50 - 200 μm  
Organization Unicellular; Flagellate 
Characteristics Red tide  
Other strain no. Other strain no. : OCH-a  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1" 
Movie  
Reference
Demura, M., Nakayama, T., Kasai, F., Kawachi, M. 2014 Genetic structure of Japanese Chattonella marina (Raphidophyceae) populations revealed using microsatellite markers. Phycol. Res., 62, 102-108.
Keywords: Chattonella marina var. antiqua; Chattonella marina var. marina; genetic structure; isolation by distance; microsatellite marker; population genetics
Strain(s): 123148384858611311411511611812116155723832384238523862387238823892390239123922393239423952396239723982399240024012402240324042405240624072509251025112512251325142515251625172518 
DOI: 10.1111/pre.12047

Kamikawa, R., Masuda, I., Demura, M., et al. 2009 Mitochondrial group II introns in the Raphidophycean flagellate Chattonella spp. suggest a diatom-to-Chattonella lateral group II intron transfer. Protist, 160, 364-375.
Keywords: Chattonella; cytochrome c oxidase subunit 1 (cox1); diatoms; group II intron; lateral transfer; Raphidophyceae
Strain(s): 123148586115118121145161462557558603671716849130323912401240224032404 
PubMed: 19346162
DOI: 10.1016/j.protis.2009.02.003

Demura, M., Noël, M.-H., Kasai, F., Watanabe, M. M., Kawachi, M. 2009 Taxonomic revision of Chattonella antiqua, C. marina and C. ovata (Raphidophyceae) based on their morphological characteristics and genetic diversity. Phycologia, 48, 518-535.
Keywords: Chattonella antiqua; Chattonella marina; Chattonella ovata; COI; COX1; Genetic diversity; ITS; Microsatellite; Morphology; Phylogeny; Population; Raphidophyceae; rbcL; Speciation; Taxonomy
Strain(s): 1235691014838485113114116118145146161293557561603671716849238323842385238623872388238923912392239323942395239623972398239924002401240224032404240524062407 
DOI: 10.2216/08-98.1

Demura, M., Kawachi, M., Kunugi, M., Nishizawa, T., Kasai, F., Watanabe, M. M. 2007 Development of microsatellite markers for the red tide-forming harmful species Chattonella antiqua, C. marina, and C. ovata (Raphidophyceae). Mol. Ecol. Notes, 7, 315-317.
Keywords: Chattonella; harmful algal bloom; microsatellite; phytoplankton; Raphidophyceae; red tide
Strain(s): 2 
DOI: 10.1111/j.1471-8286.2006.01592.x

Kamikawa, R., Masuda, I., Oyama, K., Yoshimatsu, S., Sako, Y. 2007 Genetic variation in mitochondrial genes and intergenic spacer region in harmful algae Chattonella species. Fish. Sci., 73, 871-880.
Keywords: Chattonella sp.; cytochrome c oxidase subunit 1 (cox1); cytochrome c oxidase subunit 2 (cox2); genetic diversity; harmful algae; mitochondria
Strain(s): 123148586115118121161557558603671849 
DOI: 10.1111/j.1444-2906.2007.01408.x

Nakamura, Y. 1998 Growth and grazing of a large heterotrophic dinoflagellate, Noctiluca scintillans, in laboratory cultures. J. Plankton Res., 20, 1711-1720
Strain(s): 27440 
DOI: 10.1093/plankt/20.9.1711

Hiroishi, S., Uchida, A., Nagasaki, K., Ishida, Y. 1988 A new method for identification of inter- and intra-species of the red tide algae Chattonella antiqua and Chattonella marina (Raphidophyceae) by means of monoclonal antibodies. J. Phycol., 24, 442-444.
Keywords: Chattonella; identification; immunofluorescence; inter-species; intra-species; method; monoclonal antibody; red tide
Strain(s): 121483848586113114115116118121 
DOI: 10.1111/j.1529-8817.1988.tb04488.x

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