Strain number NIES-122  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Chlamydomonas pulsatilla Wollenweber  
Synonym  
Former name  
Common name Green alga  
Locality (Date of collection) Muroran Hokkaido Japan (1966-05-12)  
Latitude / Longitude 42.36656 / 140.98844 
Habitat (Isolation source) Freshwater (Lake water)  
History < IAM (1983);  
Isolator (Date of isolation) Ichimura, Terunobu (1966-05-12)  
Identified by Ichimura, Terunobu  
State of strain Subculture; Unialgal; Clonal; Axenic[2017 Dec]  
Culture condition
(Preculture condition)
Medium:  P35  
Temperature:  20 (25) C
Light intensity:  2 (14-16) µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M (14 D)  
Gene information 18S rRNA ( AB001039 ) , psaB ( AB451200 ) , rbcL ( AB003427 )  
Cell size (min - max)  
Organization Unicellular; Flagellate 
Characteristics Phototaxis  
Other strain no. Other collection strain no. : IAM C-385 (=C-561)
Other strain no. : MKF-50  
Remarks Axenic 
Movie  
Reference
Nakada, T., Nozaki, H. 2009 Taxonomic study of two new genera of fusiform green flagellates, Tabris gen. nov. and Hamakko gen. nov. (Volvocales, Chlorophyceae). J. Phycol., 45, 482-492.
Keywords: Chlorogonium; Hamakko gen. nov.; molecular phylogeny; Tabris gen. nov.; taxonomy; ultrastructure; Volvocales
Strain(s): 122637748752760968135813591849185018692293229422954125 
PubMed: 27033826
DOI: 10.1111/j.1529-8817.2009.00652.x

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Iwahori, K., Miyata, N., Umeda, Y., Aoyama, K., Shimoi, K., Kinae, N. 1999 Applicability of environmental microorganisms to alkaline single cell gel electrophoresis (comet) assay. Jpn. J. Water Treat. Biol., 35, 261-270 (in Japanese with English summary).
Keywords: comet assay; DNA damage; environmental microorganisms; Euglena gracilis; cell lysis; unwinding; electrophoresis; SDS; γ-irradiation
Strain(s): 49122403 

Ichimura, T. 1997 Chlamydomonas pulsatilla Wollenweber from a supra-littoral rockpool on a small island offshore the city of Muroran, Hokkaido, Japan. Algol. Stud., 85, 23-29.
Keywords: Chlamydomonas pulsatilla; euryhaline species; acetate; nutrition; supra-littoral rockpool
Strain(s): 122 

Watanabe, Y., Ohmura, N., Saiki, H. 1992 Microbial CO2 fixation. 2. Isolation and determination of cultural characteristics of Chlorella strains which function under CO2 enriched atmosphere. CRIEPI Kenkyû-Hôkoku, U92014, 1-21 (in Japanese with English summary).
Keywords: climate change; CO2; CO2 fixation; microalgae; Chlorella
Strain(s): 27459294122158204226227 

Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title).
Strain(s): 1819212426293139404144454650737475767780818788899091939598991001011201221311351371441512042072132572633794152219223522392579 

Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 (in Japanese without English title).
Strain(s): 19212223242728303132333436373839404144454647484950122123127131144152173175179180181182183185198200203206217226227241266267268271295309310334338379436448450455527 

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