The Microbial Culture Collection at the National Institute for Environmental Studies

Strain Data


Strain number NIES-564  
Phylum Chlorophyta   Class Chlorophyceae 
Scientific name Astrephomene perforata Nozaki  
Synonym -----  
Former name  
Common name Green alga  
Locality (Date of collection) Hayama Hayama Kanagawa Japan (1980-12-04)  
Habitat (Isolation source) Freshwater (Paddy soil)  
History < Nozaki, Hisayoshi  
Isolator (Date of isolation) Nozaki, Hisayoshi (1981-06-20)  
Identified by Nozaki, Hisayoshi  
State of strain Unialgal; Clonal; Non-axenic  
Preservation state
(State of preservation)
Subculture  
Culture condition
( Preculture condition )
Medium:  VTAC  
Temperature: 20 C
Light intensity: 22-27 µmol photons/m2/sec     L/D cycle: 12L:12D
Duration: 1 M  
Gene information atpB ( AB014024 ) , psaA ( AB044236 AB044237 AB044238 ) , psaB ( AB044460 ) , psbC ( AB044518 AB044519 ) , rbcL ( D63429 )
Cell size (min - max)   Organization Colonial; Flagellate 
Characteristics Authentic strain; Heterothallic; Isogamy; Mating type(+); Crosses with NIES-565  
Other strain no. Other collection strain no. : UTEX 2474
Other strain no. : 1620-3-2  
Remarks  

Reference
653)   Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. , Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection , Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195196197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

756)   Nakazawa, A., Nishii, I. 2012 Amidic and acetonic cryoprotectants improve cryopreservation of volvocine green algae. CryoLetters, 33, 201-212. , Keywords: cryopreservation; dimethylformamide; hydroxyacetone; Volvocaceae; Volvox; Chlamydomonas , Strain(s): 418468545564574717721728729730731877133113611710 

786)   Nozaki, H. 1983 Morphology and taxonomy of two species of Astrephomene (Chlorophyta, Volvocales) in Japan. J. Jpn. Bot., 58, 345-352, pls. 11-13. , Strain(s): 418419564565 

811)   Nozaki, H., Ito, M., Sano, R., Uchida, H., Watanabe, M. M., Kuroiwa, T. 1995 Phylogenetic relationships within the colonial Volvocales (Chlorophyta) inferred from rbcL gene sequence data. J. Phycol., 31, 970-979. , Keywords: Chlorophyta; Goniaceae; large subunit of ribulose-1,5-bisphosphate carboxylase / oxygenase (rbcL) gene; molecular phylogeny; Tetrabaenaceae; Volvocaceae , Strain(s): 418421456460541564566569571572574577653725731732734735737895 

829)   Nozaki, H., Misawa, K., Kajita, T., Kato, M., Nohara, S., Watanabe, M. M. 2000 Origin and evolution of the colonial Volvocales (Chlorophyceae) as inferred from multiple, chloroplast gene sequences. Mol. Phylog. Evol., 17, 256-268. , Strain(s): 418456460474541545564566569571572574577582653654666720721722724725727728730731732734735736737851854872882884886887888889890893895896898 

834)   Nozaki, H., Ohta, N., Takano, H., Watanabe, M. M. 1999 Reexamination of phylogenetic relationships within the colonial Volvocales (Chlorophyta): an analysis of atpB and rbcL gene sequences. J. Phycol., 35, 104-112. , Keywords: atpB gene; Chlorophyta; Gonium; molecular phylogeny; rbcL gene; Volvocaceae; volvocine series; Volvox , Strain(s): 418456460541564566568569571572574577582653654666720721722724725727728730731732734735736737851852858861882884895 

836)   Nozaki, H., Onishi, K., Morita, E. 2002 Differences in pyrenoid morphology are correlated with differences in the rbcL genes of members of the Chloromonas lineage (Volvocales, Chlorophyceae). J. Mol. Evol., 55, 414-430. , Keywords: ATP synthase β-subunit (atpB) gene; Chloromonas; gene evolution; large subunit of Rubisco (rbcL) gene; morphological evolution; P700 chlorophyll a-apoprotein A2 (psaB) gene; pyrenoid; Volvocales , Strain(s): 96123144213418421425428432439446447456474522564566567569571574582653732851858861875882883968