Strain number NIES-42  
Phylum Cyanophyta  
Class Cyanophyceae  
Scientific name Aphanothece elabens (Brébisson ex Meneghini) Elenkin  
Synonym  
Former name Microcystis elabens  
Common name Blue-green alga ; Cyanobacteria  
Locality (Date of collection) Lake Kasumigaura Ibaraki Japan (1974-08-**)  
Latitude / Longitude 36.0428477398 / 140.373233439 
Habitat (Isolation source) Freshwater (Lake water)  
History < IAM (1983);  
Isolator (Date of isolation) Watanabe, Makoto M. (1974-08-**)  
Identified by Otsuka, Shigeto  
State of strain Cryopreservation; Unialgal; Clonal; Axenic  
Culture condition
(Preculture condition)
Medium:  CT  
Temperature:  25 C
Light intensity:  55-70 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  5 D  
Gene information 16S rRNA ( AB001724 )  
Cell size (min - max)  
Organization  
Characteristics Cyanobacterial water bloom (aoko)  
Other strain no. Other collection strain no. : IAM M-177
 
Remarks Not available; Cryopreserved; Axenic 
Movie  
Reference
Curren, E., Teruaki, Y., Kuwahara, V. S., Leong, S. C. Y. 2019 Rapid profiling of tropical marine cyanobacterial communities. Reg. Stud. Mar. Sci., 25, 100485 (article ID).
Keywords: Cyanobacteria; MinION-Oxford Nanopore sequencer; Biodiversity; 16S rRNA amplicon sequencing; Long-read sequencing; Coastal waters; Southeast Asia; Monitoring
Strain(s): 22254284321352481 
DOI: 10.1016/j.rsma.2018.100485

Pan, H., Song, L., Liu, Y., Börner, T. 2002 Detection of hepatotoxic Microcystis strains by PCR with intact cells from both culture and environmental samples. Arch. Microbiol., 178, 421-427.
Keywords: Cyanobacteria; Microcystis; Microcystin; Toxin; Peptide synthetase; Whole-cell PCR
Strain(s): 429098101595610 
PubMed: 12420161
DOI: 10.1007/s00203-002-0464-9

Metcalf, J. S., Bell, S. G., Codd, G. A. 2000 Production of novel polyclonal antibodies against the cyanobacterial toxin microcystin-LR and their application for the detection and quantification of microcystins and nodularin. Water Res., 34, 2761-2769.
Keywords: cyanobacteria; blue-green algae; microcystin; toxins; antibodies; immunoassays
Strain(s): 42102103104595 
DOI: 10.1016/S0043-1354(99)00429-7

Ward, C. J., Beattie, K. A., Lee, E. Y. C., Codd, G. A. 1997 Colorimetric protein phosphatase inhibition assay of laboratory strains and natural blooms of cyanobacteria: comparisons with high-performance liquid chromatographic analysis for microcystins. FEMS Mircobiol. Lett., 153, 465-473.
Keywords: Microcystin; Protein phosphatase; Cyanobacteria; High-performance liquid chromatography
Strain(s): 42102103104298595 
PubMed: 9271876
DOI: 10.1111/j.1574-6968.1997.tb12611.x

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