株番号 NIES-101  
門名 Cyanophyta  
綱名 Cyanophyceae 
学名 Microcystis aeruginosa (Kützing) Lemmermann  
シノニム Microcystis ichthyoblabe Kützing; Microcystis novacekii (Komárek) Compère; Microcystis viridis (A.Brown) Lemmermann; Microcystis wesenbergii Komárek  
以前使われていた種名 Microcystis aeruginosa (Kützing) Lemmermann f. aeruginosa  
一般名 藍藻 ; シアノバクテリア  
地名(採集年月) 日本 長野県 諏訪湖 (1982-10-**)  
緯度/経度 36.04954369 / 138.08476235 
生息環境(分離源) 淡水 (水)  
分離後の履歴 ← TAC;  
分離者(分離年月) 渡辺真之 (1982-10-**)  
同定者 渡辺真之  
株の状態 凍結保存; 非無菌; 単藻; クローン  
培養条件
(前培養条件)
培地名:  CB  
温度: 25 ℃
光強度: 20-30 µmol photons/m2/sec,  明暗周期: 10L:14D
継代培養周期: 20 D  
遺伝子情報 16S rRNA ( AB023260 ) , ITS ( AB333807 ) , ftsZ ( AB324858 ) , glnA ( AB324937 ) , gltX ( AB325016 ) , gyrB ( AB325095 ) , pgi ( AB325174 ) , recA ( AB325253 ) , rpoC1 ( AB333818 ) , tpi ( AB325332 )  
特性 アオコ ; 生物指標 ; ST9 (Tanabe, Y. et al. 2007)  
サイズ (min - max)  
体制  
その他の株番号 その他の株番号:TAC 48 (S-TAN-48)  
注意事項 凍結保存  
動画  
文献
Sun, L.W., Jiang, W.J., Sato, H., Kawachi, M., Lu, X.W. 2016 Rapid classification and identification of Microcystis aeruginosa strains using MALDI-TOF MS and polygenetic analysis. PLoS One, 11, e0156275 (article ID).
Strain(s): 44878889901001011041051071091111124786048439011043105310581061106810691070107210761077108510941099110111041105111311151126113411351144115111571170121112131215121612171218122212241230123212331239 
DOI: 10.1371/journal.pone.0156275

Luu, P. T., Son, D. T., Utsumi, M. 2016 Accumulation and elimination of Microcystins in freshwater clam Corbicula leana under laboratory conditions, assessment of risks to humans. J. Biotechnol., 14, 1-10.
キーワード: alga; heterokontophyte; hexa-amine; labyrinthulomycete; methylated polyamine; penta-amine
Strain(s): 1018001086 

Pham, T., Shimizu, K., Dao, T., Hong-Do, L., Utsumi, M. 2015 Microcystin uptake and biochemical responses in the freshwater clam Corbicula leana P. exposed to toxic and non-toxic Microcystis aeruginosa: Evidence of tolerance to cyanotoxins. Toxicology Report, 2, 88-98.
キーワード: Bioaccumulation; Biochemical response; Corbicula leana; Microcystins; Toxic and non-toxic
Strain(s): 10110862170 
DOI: 10.1016/j.toxrep.2015.01.012

Kataoka, T., Homma, T., Nakano, S., Hodoki, Y., Ohbayashi, K., Kondo, R. 2013 PCR primers for selective detection of intra-species variations in the bloom-forming cyanobacterium, Microcystis. Harmful Algae, 23, 46-54.
キーワード: Microcystis aeruginosa; Quantitative PCR; 16S-23S rDNA ITS fegion; Selective detection; Intra-species variation
Strain(s): 4487909810110210310711111229860410621070 

Tanabe, Y., Watanabe, M. M. 2011 Local expansion of a panmictic lineage of water bloom-forming cyanobacterium Microcystis aeruginosa. PLoS One, 6, e17085 (article ID).
Strain(s): 448788899091989910010110210310410510610710810911011111229829947860484390110501051105210531054105510561057105810591060106110621063106410651066106710681069107010721075107610771085109010911092109310941095109610971098110011011102110311041105110611071108111311141115112211231124112511261127112811291130113111321133113411351136113711381139114011411142114311441145114611471148114911501151115211531155115611571159116011611162116311641170117111721173117411751176117711791180118111831184118511861209121112131214121512161217121812191220122112221223122412251226122712281229123012311232123312341235123612371238123912401241124212431244124512511252125312542088208920902091246524662467246824692470247124722473247424752476247724782479248024812482248324842485248624872488248924902491249224932494249524962546254725482549255025512552255325542555255625572558255925602561259125922593259425952596259725982599260026012602260326042605260626072608260926102611261226132617261826192620262126222623262426252626262726282629 

Kuno, S., Yoshida, T., Kamikawa, R., Hosoda, N., Sako, Y. 2010 The distribution of a phage-related insertion sequence element in the cyanobacterium, Microcystis aeruginosa. Microbes. Environ., 25, 295-301.
キーワード: cyanobacteria; Microcystis; cyanophage; insertion sequence; horizontal gene transfer
Strain(s): 90919899101102112298604843105810671070108610981105 

Ziemert, N., Ishida, K., Weiz, A., Hertweck, C., Dittmann, E. 2010 Exploiting the natural diversity of microviridin gene clusters for discovery of novel tricyclic depsipeptides. Appl. Environ. Microbiol., 76, 3568-3574.
Strain(s): 100101102843 

Tanabe, Y., Sano, T., Kasai, F., Watanabe, M. M. 2009b Recombination, cryptic clades and neutral molecular divergence of the microcystin synthetase (mcy) genes of toxic cyanobacterium Microcystis aeruginosa. BMC Evol. Biol., 9, 115 (article ID).
Strain(s): 44878889909198991001011021031041051061071081091101111122982994786048439011050105110521053105410551056105710581059106010611062106310641065106610671068106910701072107510761077108510901091109210931094109510961097109811001101110211031104110511061107110811131114111511221123112411251126112711281129113011311132113311341135113611371138113911401141114211431144114511461147114811491150115111521153115511561157115911601161116211631164117011711172120912111213121412151216121712181219122012211222122312241225122612271228122912301231123212331234123512361237123812391240124112421243124412451251125212531254246524662467246924702471247224732474247524762477247824792561 
DOI: 10.1186/1471-2148-9-115

Cadel-Six, S., Dauga, C., Castets, A. M., Rippka, R., Bouchier, C., de Marsac, N. T., Welker, M. 2008 Halogenase genes in nonribosomal peptide synthetase gene clusters of Microcystis (Cyanobacteria): sporadic distribution and evolution. Mol. Biol. Evol., 25, 2031-2041.
キーワード: halogenase; cyanopeptolin; aeruginosin; DNA rearrangement; secondary peptide metabolite; chlorination; internal transcribed spacer; phylogeny
Strain(s): 8998101102298 
PubMed: 18614525
DOI: 10.1093/molbev/msn150

Yoshida, M., Yoshida, T., Satomi, M., Takashima, Y., Hosoda, N., Hiroishi, S. 2008 Intra-specific phenotypic and genotypic variation in toxic cyanobacterial Microcystis aeruginosa. J. Appl. Phycol., 105, 407-415.
キーワード: API ZYM; diversity; microcystin; Microcystis; phylogeny; population; taxonomy; toxic; cyanobacteria
Strain(s): 101102103112298604105510581062107010911105 

Tanabe, Y., Kasai, F., Watanabe, M. M. 2007 Multilocus sequence typing (MLST) reveals high genetic diversity and clonal population structure of the toxic cyanobacterium Microcystis aeruginosa. Microbiology, 153, 3695-3703.
Strain(s): 44878889909198991001011021031041051061071081091101111122982994786048439019331050105110521053105410551056105710581059106010611062106310641065106610671068106910701072107510761077108510901091109210931094109510961097109811001101110211031104110511061107110811131114111511221123112411251126112711281129113011311132113311341135113611371138113911401141114211431144114511461147114811491150115111521153115511561157115911601161116211631164117011711172120912111213121412151216121712181219122012211222122312241225122612271228122912301231123212331234123512361237123812391240124112421243124412451251125212531254 

Fujii, K., Yahashi, Y., Nakano, T., Imanishi, S., Baldia, S. F., Harada, K. 2002 Simultaneous detection and determination of the absolute configuration of thiazole-containing amino acids in a peptide. Tetrahedron, 58, 6873-6879.
キーワード: microcyclamide; absolute configuration; advanced Marfey's method; thiazole-containing (TZL-) amino acid
Strain(s): 101298 

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
キーワード: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Yuki, Y., Yoshida, T., Hiroishi, S. 2002 Molecular detection of the toxic cyanobacteria [Yûdoku aoko no bunshi-shikibetsu to yosatsu he-no ôyô]. In Series of Fisheries Science Vol. 143, Prevention and Extermination Strategies of Harmful Algal Blooms [Suisan-gaku shirîzu 134, Yûgai-yûdoku sôrui burûmu no yobô to kujo] , Eds. by Hiroishi, S., Imai, I. & Ishimaru, T., Koseisha-Koseikaku, Tokyo, p. 43-53 (in Japanese without English title).
Strain(s): 738187888990919899100101102104105106108109110111112204230298299604 

Pan, H., Song, L., Liu, Y., Börner, T. 2002 Detection of hepatotoxic Microcystis strains by PCR with intact cells from both culture and environmental samples. Arch. Microbiol., 178, 421-427.
キーワード: Cyanobacteria; Microcystis; Microcystin; Toxin; Peptide synthetase; Whole-cell PCR
Strain(s): 429098101595610 

Kondo, R., Kagiya, G., Hiroishi, S., Watanabe, M. 2000 Genetic typing of a bloom-forming cyanobacterial genus Microcystis in Japan using 16S rRNA gene sequence analysis. Plankton Biol. Ecol., 47, 1-6.
キーワード: genetic typing; 16S rDNA; Microcystis; cyanobacteria; phylogenetic analysis
Strain(s): 878889909198991001011021041051061081091101111122982996041055106110681076109010911105112211421164 

Kondo, R., Kagiya, G., Komura, M., Yuki, Y., Hiroishi, S., Watanabe, M. 2000 Molecular taxonomy of a bloom-forming cyanobacterial genus Microcystis. Bull. Plankton Soc. Japan, 47, 25-33 (in Japanese with English summary).
キーワード: cyanobacteria; Microcystis; bacterial criteria; molecular analysis
Strain(s): 6878889909198991001011021041051061081091101111122982996041055106110681076109010911105112211421164 

Kondo, R., Kagiya, G., Hiroishi, S., Watanabe, M., Hata, Y. 1999 Rapid DNA extraction from a bloom-forming cyanobacterium. Microbes. Environ., 14, 157-161.
キーワード: DNA extraction; PCR; Microcystis; cyanobacteria
Strain(s): 878889909198991001011021041051061081091101111122982996041055106110681076109010911105112211421164 

Kondo, R., Kagiya, G., Yuki, Y., Hiroishi, S., Watanabe, M. 1998 Taxonomy of a bloom-forming cyanobacterial genus Microcystis. Nippon Suisan Gakkaishi, 64, 291-292 (in Japanese with English title).
Strain(s): 87888990919899100101102104105106107108109110111112298299604 

Nakamura, H. 1993 Results of AGP experiments [AGP, T-P, PO4-P no shiken oyobi bunsekikekka]. Unpublished report in Ebara Yujiraito Co. Ltd,. 32 pp. (in Japanese without English title).
Strain(s): 35101 

Watanabe, M. F., Oishi, S., Watanabe, Y., Watanabe, M. 1986 Strong probability of lethal toxicity in the blue-green alga Microcystis viridis Lemmermann. J. Phycol., 22, 552-556.
Strain(s): 981001011031122981053105410551058105910671091 

Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title).
Strain(s): 1819212426293139404144454650737475767780818788899091939598991001011201221311351371441512042072132572633794152219223522392579 

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