Strain number NIES-484  
Phylum Chlorophyta  
Class Nephroselmidophyceae  
Scientific name Nephroselmis olivacea Stein  
Synonym  
Former name  
Common name  
Locality (Date of collection) Tsuchiura Ibaraki Japan (1986-02-**)  
Latitude / Longitude 36.081096 / 140.16337 
Habitat (Isolation source) Freshwater (Pond sediment)  
History < Suda, Shoichiro  
Isolator (Date of isolation) Suda, Shoichiro (1986-05-06)  
Identified by Suda, Shoichiro  
State of strain Subculture; Unialgal; Clonal; Axenic[2017 Dec]  
Culture condition
(Preculture condition)
Medium:  AF-6  
Temperature:  20 C
Light intensity:  10-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  45 D  
Gene information Plastid DNA ( AF137379 ) , Mitochondria DNA ( AF110138 ) , eno ( AF348938 ) , ftsZ2 ( AB673439 )  
Cell size (min - max) 8 - 10 μm  
Organization Unicellular; Flagellate 
Characteristics Genome decoded strain (Turmel et al. 1999a, Turmel et al. 1999b) ; Heterothallic ; Mating type (−)  
Other strain no. Other strain no. : S-N-5-8  
Remarks Axenic 
Movie  
Reference
Hunsperger, H. M., Randhawa, T., Cattolico, R. A. 2015 Extensive horizontal gene transfer, duplication, and loss of chlorophyll synthesis genes in the algae. BMC Evol. Biol., 15, 16 (article ID).
Keywords: Chlorophyll synthesis; Horizontal gene transfer; Endosymbiotic gene transfer; Gene duplication; Algae; Protochlorophyllide
Strain(s): 29336048413632140 
PubMed: 25887237
DOI: 10.1186/s12862-015-0286-4

Rodríguez-Salinas, E., Riveros-Rosas, H., Li, ZK., Fučíková, K., Brand JJ., Lewis, LA., González-Halphen, D. 2012 Lineage-specific fragmentation and nuclear relocation of the mitochondrial cox2 gene in chlorophycean green algae (Chlorophyta). Mol. Phylogenet. Evol., 64, 166-176.
Keywords: Mitochondrial genes; Cytochrome oxidase; Chlorophyte algae; Chlorophyceae; Gene migration; Gene fragmentation
Strain(s): 360484 
PubMed: 22724135
DOI: 10.1016/j.ympev.2012.03.014

Miyagishima, S., Suzuki, K., Okazaki, K., Kabeya, Y. 2012 Expression of the nucleus-encoded chloroplast division genes and proteins regulated by the algal cell cycle. Mol. Biol. Evol., 29, 2957-2970.
Keywords: cell cycle; chloroplast division; endosymbiosis; endosymbiotic gene transfer
Strain(s): 296484547133221703377 
PubMed: 22490821
DOI: 10.1093/molbev/mss102

Laflamme, M., Lee, R. W. 2003 Mitochondrial genome conformation among CW-group Chlorophycean algae. J. Phycol., 39, 213-220.
Keywords: Chlamydomonas; Chlorophyceae; gel electrophoresis; mitochondrial DNA conformation; phylogeny
Strain(s): 296484 
DOI: 10.1046/j.1529-8817.2003.02045.x

Turmel, M., Ehara, M., Otis, C., Lemieux, C. 2002a Phylogenetic relationships among streptophytes as inferred from chloroplast small and large subunit rRNA gene sequences. J. Phycol., 38, 364-375.
Keywords: Charophyceae; chloroplast rRNA genes; introns; land plants; phylogeny; Streptophyta
Strain(s): 296484 
DOI: 10.1046/j.1529-8817.2002.01163.x

Keeling, p. J., Palmer, J. D. 2001 Lateral transfer at the gene and subgenic levels in the evolution of eukaryotic enolase. Proc. Nati. Acad. Sci. U. S. A., 98, 10745-10750.
Keywords: phylogeny; protein insertions; apicomplexa; recombination
Strain(s): 484 
PubMed: 11526220
DOI: 10.1073/pnas.191337098

Turmel, M., Otis, C., Lemieux, C. 1999b The complete chloroplast DNA sequence of the green alga Nephroselmis olivacea: Insights into the architecture of ancestral chloroplast genomes. Proc. Nati. Acad. Sci. U. S. A., 96, 10248-10253.
Strain(s): 484 
PubMed: 10468594
DOI: 10.1073/pnas.96.18.10248

Turmel, M., Lemieux, C., Burger, G., Lang, B. F., Otis, C., Plante, I., Gray, M. W. 1999a The complete mitochondrial DNA sequences of Nephroselmis olivacea and Pedinomonas minor: Two radically different evolutionary patterns within green algae. Plant Cell, 11, 1717-1729.
Strain(s): 484 
PubMed: 10488238
DOI: 10.1105/tpc.11.91717

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