A big image opens when the thumbnail is clicked.(nies-0483.jpg)
Strain number NIES-483  
Phylum Chlorophyta  
Class Nephroselmidophyceae  
Scientific name Nephroselmis olivacea Stein  
Synonym  
Former name  
Common name  
Locality (Date of collection) Tsuchiura Ibaraki Japan (1986-02-**)  
Latitude / Longitude 36.081096 / 140.16337 
Habitat (Isolation source) Freshwater (Pond sediment)  
History < Suda, Shoichiro  
Isolator (Date of isolation) Suda, Shoichiro (1986-05-09)  
Identified by Suda, Shoichiro  
State of strain Subculture; Unialgal; Clonal; Axenic[2017 Dec]  
Culture condition
(Preculture condition)
Medium:  AF-6  
Temperature:  20 C
Light intensity:  10-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M  
Gene information 18S rRNA ( FN562436 ) , 18S-ITS1-5.8S-ITS2-28S ( HE610146 ) , 16S-ITS1-tRNA-Ile-tRNA-Ala-23S ( FN563086 ) , beta-tubulin ( AB491622 ) , psbO ( AB293978 )  
Cell size (min - max) - 17 μm  
Organization Unicellular; Flagellate 
Characteristics Heterothallic ; Mating type (+)  
Other strain no. Other strain no. : S-N-2-1  
Remarks Axenic 
Movie  
Reference
Marin, B. 2012 Nested in the Chlorellales or independent class? Phylogeny and classification of the Pedinophyceae (Viridiplantae) revealed by molecular phylogenetic analyses of complete nuclear and plastid-encoded rRNA operons. Protist, 163, 778-805.
Keywords: Marsupiomonadales; Marsupiomonas; Pedinomonas; Pedinophyceae; Resultor; Resultomonas
Strain(s): 252483 
PubMed: 22192529
DOI: 10.1016/j.protis.2011.11.004

Marin, B., Melkonian, M. 2010 Molecular phylogeny and classification of the Mamiellophyceae class. nov. (Chlorophyta) based on sequence comparisons of the nuclear- and plastid-encoded rRNA operons. Protist, 161, 304-336.
Keywords: prasinophytes; SSU rDNA; LSU rDNA; ITS2; systematics; freshwater; Monomastigales; Dolichomastigales; Mamiellales; Monomastigaceae; Dolichomastigaceae; Crustomastigaceae; Bathycoccaceae
Strain(s): 252255360483 
PubMed: 20005168
DOI: 10.1016/j.protis.2009.10.002

Takahashi, F., Okabe, Y., Nakada, T., Sekimoto, H., Ito, M., Kataoka, H. & Nozaki, H. 2007 Origins of the secondary plastids of Euglenophyta and Chlorarachniophyta as revealed by an analysis of the plastid-targeting, nuclear-encoded gene psbO. J. Phycol., 43, 1302-1309.
Keywords: alanine-X-alanine motif; Chlorarachniophyta; Euglenophyta; phylogeny; plastids; psbO; secondary endosymbiosis; transit peptide; thylakoid-targeting domain
Strain(s): 67203254381483533624656 
DOI: 10.1111/j.1529-8817.2007.00411.x

Nowack, E. C. M., Podola, B., Melkonian, M. 2005 The 96-well twin-layer system: a novel approach in the cultivation of microalgae. Protist, 156, 239-251.
Keywords: cultivation; culture collections; cell immobilisation; microalgae; microtiter plates; filter plates
Strain(s): 255483485 
PubMed: 16171190
DOI: 10.1016/j.protis.2005.04.003

Yoshii, Y., Takaichi, S., Maoka, T., Suda, S., Sekiguchi, H., Nakayama, T., Inouye, I. 2005 Variation of siphonaxanthin series among the genus Nephroselmis (Prasinophyceae, Chlorophyta), including a novel primary methoxy carotenoid. J. Phycol., 41, 827-834.
Keywords: carotenoids; methylation; Nephroselmis; photosynthetic antenna system; Prasinophyceae; siphonaxanthin series
Strain(s): 2524834869349352309 
DOI: 10.1111/j.1529-8817.2005.00103.x

Hamana, K., Aizaki T., Arai, E., Saito, A., Uchikata, K., Ohnishi, H. 2004 Distribution of norspermidine s a cellular polyamine within micro green algae including non-photosynthetic achlorophyllous Polytoma, Polytomella, Prototheca and Helicosporidium. J. Gen. Appl. Microbiol., 50, 289-295.
Keywords: Chlorophyta; green alga; Helicosporidium; norspermidine; polyamine; Polytoma; Polytomella; Prototheca
Strain(s): 18251254255296329483486536 

Suda, S., Watanabe, M. M., Inouye, I. 2004 Electron microscopy of sexual reproduction in Nephroselmis olivacea (Prasinophyceae, Chlorophyta). Phycol. Res., 52, 273-283.
Keywords: electron microscopy; endoplasmic reticulum; germination; Nephroselmis olivacea; Prasinophyceae; sexual reproduction; zygote formation; zygote wall
Strain(s): 483485 
DOI: 10.1111/j.1440-183.2004.00346.x

Marin, B., Melkonian, M. 1994 Flagellar hairs in prasinophytes (Chlorophyta): ultrastructure and distribution on the flagellar surface. J. Phycol., 30, 659-678.
Keywords: flagellar hairs; Mamiella; Mantoniella; Nephroselmis; Prasinophyceae; Pseudoscourfieldia; Pterosperma; Pyramimonas; Scherffelia; ultrastructure
Strain(s): 221483485 
DOI: 10.1111/j.0022-3646.1994.00659.x

Suda, S., Watanabe, M. M., Inouye, I. 1989 Evidence for sexual reproduction in the primitive green alga Nephroselmis olivacea (Prasinophyceae). J. Phycol., 25, 596-600.
Strain(s): 483485 
DOI: 10.1111/j.1529-8817.1989.tb00266.x

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