Strain Data

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Strain number		NIES-483
Phylum		Chlorophyta
Class		Nephroselmidophyceae
Scientific name		Nephroselmis olivacea Stein
Synonym
Former name
Common name
Locality (Date of collection)		Tsuchiura Ibaraki Japan (1986-02-**)
Latitude / Longitude		36.081096 / 140.16337
Habitat (Isolation source)		Freshwater (Pond sediment)
History		< Suda, Shoichiro
Isolator (Date of isolation)		Suda, Shoichiro (1986-05-09)
Identified by		Suda, Shoichiro
State of strain		Subculture; Unialgal; Clonal; Axenic[2017 Dec]
Culture condition (Preculture condition)		Medium: AF-6 Temperature: 20 C Light intensity: 10-15 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 2 M
Gene information		18S rRNA ( FN562436 ) , 18S-ITS1-5.8S-ITS2-28S ( HE610146 ) , 16S-ITS1-tRNA-Ile-tRNA-Ala-23S ( FN563086 ) , beta-tubulin ( AB491622 ) , psbO ( AB293978 )
Cell size (min - max)		- 17 μm
Organization		Unicellular; Flagellate
Characteristics		Heterothallic ; Mating type (+)
Other strain no.		Other strain no. : S-N-2-1
Remarks		Axenic
Movie

Reference
Marin, B. 2012 Nested in the Chlorellales or independent class? Phylogeny and classiﬁcation of the Pedinophyceae (Viridiplantae) revealed by molecular phylogenetic analyses of complete nuclear and plastid-encoded rRNA operons. Protist, 163, 778-805. Keywords: Marsupiomonadales; Marsupiomonas; Pedinomonas; Pedinophyceae; Resultor; Resultomonas Strain(s): 252, 483 PubMed: 22192529 DOI: 10.1016/j.protis.2011.11.004 Marin, B., Melkonian, M. 2010 Molecular phylogeny and classiﬁcation of the Mamiellophyceae class. nov. (Chlorophyta) based on sequence comparisons of the nuclear- and plastid-encoded rRNA operons. Protist, 161, 304-336. Keywords: prasinophytes; SSU rDNA; LSU rDNA; ITS2; systematics; freshwater; Monomastigales; Dolichomastigales; Mamiellales; Monomastigaceae; Dolichomastigaceae; Crustomastigaceae; Bathycoccaceae Strain(s): 252, 255, 360, 483 PubMed: 20005168 DOI: 10.1016/j.protis.2009.10.002 Takahashi, F., Okabe, Y., Nakada, T., Sekimoto, H., Ito, M., Kataoka, H. & Nozaki, H. 2007 Origins of the secondary plastids of Euglenophyta and Chlorarachniophyta as revealed by an analysis of the plastid-targeting, nuclear-encoded gene psbO. J. Phycol., 43, 1302-1309. Keywords: alanine-X-alanine motif; Chlorarachniophyta; Euglenophyta; phylogeny; plastids; psbO; secondary endosymbiosis; transit peptide; thylakoid-targeting domain Strain(s): 67, 203, 254, 381, 483, 533, 624, 656 DOI: 10.1111/j.1529-8817.2007.00411.x Nowack, E. C. M., Podola, B., Melkonian, M. 2005 The 96-well twin-layer system: a novel approach in the cultivation of microalgae. Protist, 156, 239-251. Keywords: cultivation; culture collections; cell immobilisation; microalgae; microtiter plates; filter plates Strain(s): 255, 483, 485 PubMed: 16171190 DOI: 10.1016/j.protis.2005.04.003 Yoshii, Y., Takaichi, S., Maoka, T., Suda, S., Sekiguchi, H., Nakayama, T., Inouye, I. 2005 Variation of siphonaxanthin series among the genus Nephroselmis (Prasinophyceae, Chlorophyta), including a novel primary methoxy carotenoid. J. Phycol., 41, 827-834. Keywords: carotenoids; methylation; Nephroselmis; photosynthetic antenna system; Prasinophyceae; siphonaxanthin series Strain(s): 252, 483, 486, 934, 935, 2309 DOI: 10.1111/j.1529-8817.2005.00103.x Hamana, K., Aizaki T., Arai, E., Saito, A., Uchikata, K., Ohnishi, H. 2004 Distribution of norspermidine s a cellular polyamine within micro green algae including non-photosynthetic achlorophyllous Polytoma, Polytomella, Prototheca and Helicosporidium. J. Gen. Appl. Microbiol., 50, 289-295. Keywords: Chlorophyta; green alga; Helicosporidium; norspermidine; polyamine; Polytoma; Polytomella; Prototheca Strain(s): 18, 251, 254, 255, 296, 329, 483, 486, 536 Suda, S., Watanabe, M. M., Inouye, I. 2004 Electron microscopy of sexual reproduction in Nephroselmis olivacea (Prasinophyceae, Chlorophyta). Phycol. Res., 52, 273-283. Keywords: electron microscopy; endoplasmic reticulum; germination; Nephroselmis olivacea; Prasinophyceae; sexual reproduction; zygote formation; zygote wall Strain(s): 483, 485 DOI: 10.1111/j.1440-183.2004.00346.x Marin, B., Melkonian, M. 1994 Flagellar hairs in prasinophytes (Chlorophyta): ultrastructure and distribution on the flagellar surface. J. Phycol., 30, 659-678. Keywords: flagellar hairs; Mamiella; Mantoniella; Nephroselmis; Prasinophyceae; Pseudoscourfieldia; Pterosperma; Pyramimonas; Scherffelia; ultrastructure Strain(s): 221, 483, 485 DOI: 10.1111/j.0022-3646.1994.00659.x Suda, S., Watanabe, M. M., Inouye, I. 1989 Evidence for sexual reproduction in the primitive green alga Nephroselmis olivacea (Prasinophyceae). J. Phycol., 25, 596-600. Strain(s): 483, 485 DOI: 10.1111/j.1529-8817.1989.tb00266.x

Reference

Marin, B. 2012 Nested in the Chlorellales or independent class? Phylogeny and classiﬁcation of the Pedinophyceae (Viridiplantae) revealed by molecular phylogenetic analyses of complete nuclear and plastid-encoded rRNA operons. Protist, 163, 778-805.
Keywords: Marsupiomonadales; Marsupiomonas; Pedinomonas; Pedinophyceae; Resultor; Resultomonas
Strain(s): 252, 483
PubMed: 22192529
DOI: 10.1016/j.protis.2011.11.004

Marin, B., Melkonian, M. 2010 Molecular phylogeny and classiﬁcation of the Mamiellophyceae class. nov. (Chlorophyta) based on sequence comparisons of the nuclear- and plastid-encoded rRNA operons. Protist, 161, 304-336.
Keywords: prasinophytes; SSU rDNA; LSU rDNA; ITS2; systematics; freshwater; Monomastigales; Dolichomastigales; Mamiellales; Monomastigaceae; Dolichomastigaceae; Crustomastigaceae; Bathycoccaceae
Strain(s): 252, 255, 360, 483
PubMed: 20005168
DOI: 10.1016/j.protis.2009.10.002

Takahashi, F., Okabe, Y., Nakada, T., Sekimoto, H., Ito, M., Kataoka, H. & Nozaki, H. 2007 Origins of the secondary plastids of Euglenophyta and Chlorarachniophyta as revealed by an analysis of the plastid-targeting, nuclear-encoded gene psbO. J. Phycol., 43, 1302-1309.
Keywords: alanine-X-alanine motif; Chlorarachniophyta; Euglenophyta; phylogeny; plastids; psbO; secondary endosymbiosis; transit peptide; thylakoid-targeting domain
Strain(s): 67, 203, 254, 381, 483, 533, 624, 656
DOI: 10.1111/j.1529-8817.2007.00411.x

Nowack, E. C. M., Podola, B., Melkonian, M. 2005 The 96-well twin-layer system: a novel approach in the cultivation of microalgae. Protist, 156, 239-251.
Keywords: cultivation; culture collections; cell immobilisation; microalgae; microtiter plates; filter plates
Strain(s): 255, 483, 485
PubMed: 16171190
DOI: 10.1016/j.protis.2005.04.003

Yoshii, Y., Takaichi, S., Maoka, T., Suda, S., Sekiguchi, H., Nakayama, T., Inouye, I. 2005 Variation of siphonaxanthin series among the genus Nephroselmis (Prasinophyceae, Chlorophyta), including a novel primary methoxy carotenoid. J. Phycol., 41, 827-834.
Keywords: carotenoids; methylation; Nephroselmis; photosynthetic antenna system; Prasinophyceae; siphonaxanthin series
Strain(s): 252, 483, 486, 934, 935, 2309
DOI: 10.1111/j.1529-8817.2005.00103.x

Hamana, K., Aizaki T., Arai, E., Saito, A., Uchikata, K., Ohnishi, H. 2004 Distribution of norspermidine s a cellular polyamine within micro green algae including non-photosynthetic achlorophyllous Polytoma, Polytomella, Prototheca and Helicosporidium. J. Gen. Appl. Microbiol., 50, 289-295.
Keywords: Chlorophyta; green alga; Helicosporidium; norspermidine; polyamine; Polytoma; Polytomella; Prototheca
Strain(s): 18, 251, 254, 255, 296, 329, 483, 486, 536

Suda, S., Watanabe, M. M., Inouye, I. 2004 Electron microscopy of sexual reproduction in Nephroselmis olivacea (Prasinophyceae, Chlorophyta). Phycol. Res., 52, 273-283.
Keywords: electron microscopy; endoplasmic reticulum; germination; Nephroselmis olivacea; Prasinophyceae; sexual reproduction; zygote formation; zygote wall
Strain(s): 483, 485
DOI: 10.1111/j.1440-183.2004.00346.x

Marin, B., Melkonian, M. 1994 Flagellar hairs in prasinophytes (Chlorophyta): ultrastructure and distribution on the flagellar surface. J. Phycol., 30, 659-678.
Keywords: flagellar hairs; Mamiella; Mantoniella; Nephroselmis; Prasinophyceae; Pseudoscourfieldia; Pterosperma; Pyramimonas; Scherffelia; ultrastructure
Strain(s): 221, 483, 485
DOI: 10.1111/j.0022-3646.1994.00659.x

Suda, S., Watanabe, M. M., Inouye, I. 1989 Evidence for sexual reproduction in the primitive green alga Nephroselmis olivacea (Prasinophyceae). J. Phycol., 25, 596-600.
Strain(s): 483, 485
DOI: 10.1111/j.1529-8817.1989.tb00266.x

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