Strain number NIES-476  
Phylum Streptophyta  
Class Mesostigmatophyceae  
Scientific name Mesostigma viride Lauterborn  
Synonym  
Former name  
Common name  
Locality (Date of collection) Yoshino Park Mitsukaido Ibaraki Japan (1986-01-17)  
Latitude / Longitude 36.075731 / 140.001211 
Habitat (Isolation source) Freshwater (Pond sediment)  
History < Suda, Shoichiro  
Isolator (Date of isolation) Suda, Shoichiro (1986-12-13)  
Identified by Suda, Shoichiro  
State of strain Subculture; Unialgal; Clonal; Axenic[2017 Dec]  
Culture condition
(Preculture condition)
Medium:  CT  
Temperature:  20 C
Light intensity:  10-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information CO1 ( HQ668013 ) , GAPDH ( HQ668009 ) , HDS mRNA ( EF119229 ) , MDD mRNA ( EF119233 ) , HMGS mRNA ( EF119231 ) , MECPS mRNA ( EF119228 ) , MCT mRNA ( EF119226 ) , DXS mRNA ( EF119224 ) , HMGR mRNA ( EF119232 ) , HDR mRNA ( EF119230 ) , CMK mRNA ( EF119227 ) , DXR mRNA ( EF119225 ) , psbO ( DN255652 )  
Cell size (min - max) - 16 μm  
Organization Unicellular; Flagellate 
Characteristics Heterothallic ; Mating type (−)  
Other strain no. Other strain no. : KY-Mes-1  
Remarks Unstable; Fragile species to transportation stresses; Read and agree "How to order 4.1"; Axenic 
Movie  
Reference
Hua, JM., Smith, D. R., Borza, T., Lee, R. W. 2012 Similar relative mutation rates in the three genetic compartments of Mesostigma and Chlamydomonas. Protist, 163, 105-115.
Keywords: Chlamydomonas; genome architecture; Mesostigma; mutation rate; silent substitution rate
Strain(s): 296476 
PubMed: 21621456
DOI: 10.1016/j.protis.2011.04.003

Buchmanna, K., Becker, B. 2009 The system of contractile vacuoles in the green alga Mesostigma viride (Streptophyta). Protist, 160, 427-443.
Keywords: Mesostigma viride; Streptophyta; contractile vacuole; 3-D reconstruction; transmission electron microscopy
Strain(s): 476 
PubMed: 19356977
DOI: 10.1016/j.protis.2009.01.002

Grauvogel, C., Petersen, J. 2007 Isoprenoid biosynthesis authenticates the classification of the green alga Mesostigma viride as an ancient streptophyte. Gene, 396(1), 125-133.
Keywords: evolution; primer design; cytosolic MVA pathway; plastid MEP pathway; Chlorophyta; Plantae; Cyanophora paradoxa
Strain(s): 476 
PubMed: 17433859
DOI: 10.1016/j.gene.2007.02.020

Simon, A., Glockner, G., Felder, M., Melkonian, M., Becker, B. 2006 EST analysis of the scaly green flagellate Mesostigma viride (Streptophyta): implications for the evolution of green plants (Viridiplantae). BMC Plant Biol., 6, 2 (article ID).
Strain(s): 476 
PubMed: 16476162
DOI: 10.1186/1471-2229-6-2

Matsunaga, S., Watanabe, S., Sakaushi, S., Miyamura, S., Hori, T. 2003 Screening effect diverts the swimming directions from diaphototactic to positive phototactic in a disk-shaped green flagellate Mesostigma viride. Photochem. Photobiol., 77, 324-332.
Strain(s): 476477 
PubMed: 12685662
DOI: 10.1562/0031-8655(2003)077<0324:SEDTSD>2.0.CO;2

Yoshii, Y., Takaichi, S., Maoka, T., Inouye, I. 2003 Photosynthetic pigment composition in the primitive green alga Mesostigma viride (Prasinophyceae): phylogenetic and evolutionary implications. J. Phycol., 39, 570-576.
Keywords: abscisic acid; carotenoid; Mesostigma; neoxanthin; siphonaxanthin; siphonaxanthin ester
Strain(s): 296475476477 
DOI: 10.1046/j.1529-8817.2003.02098.x

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