The Microbial Culture Collection at the National Institute for Environmental Studies

Strain Data


Strain number NIES-641  
Phylum Chlorophyta   Class Trebouxiophyceae 
Scientific name Chlorella vulgaris Beijerinck var. vulgaris  
Synonym -----  
Former name  
Common name  
Locality (Date of collection) Miyata River Ibaraki Japan (1987-02-25)  
Habitat (Isolation source) Freshwater (River water)  
History < Kasai, Fumie  
Isolator (Date of isolation) Kasai, Fumie (1987-03-23)  
Identified by Kasai, Fumie; Confirmed at NIES by DNA sequencing  
State of strain Unialgal; Clonal; Axenic  
Preservation state
(State of preservation)
Cryopreservation  
Culture condition
( Preculture condition )
Medium:  C  
Temperature: 20 C
Light intensity: 8-15 µmol photons/m2/sec     L/D cycle: 12L:12D
Duration: 6 M  
Gene information 18S rRNA ( AB488576 )
Cell size (min - max)   Organization Unicellular; Non-flagellate 
Characteristics  
Other strain no. Other strain no. : 1st-3-26  
Remarks Cryopreserved 

Reference
555)   Lu, J., Takeuchi, T., Satoh, H. 2004 Ingestion and assimilation of three species of freshwater algae by larval tilapia Oreochromis niloticus. Aquaculture, 238, 437-449. , Keywords: carbon assimilation; closed ecological recirculating aquaculture system (CERAS); Chlorella; 14C labeling; Euglena; ingestion; Oreochromis niloticus; Spirulina , Strain(s): 3948641 

653)   Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. , Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection , Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195196197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

1162)   Takamura, N., Kasai, F., Watanabe, M. M. 1988 Differences in the tolerant level of benthic algae to heavy metal - The effects of Cu, Cd, and Zn on the photosynthesis. Res. Rep. Natl. Inst. Environ. Stud., No. 114, 223-232 (in Japanese with English summary). , Strain(s): 71305329411412413434504531538641642662 

1163)   Takamura, N., Kasai, F., Watanabe, M. M. 1989 Effects of Cu, Cd and Zn on photosynthesis of freshwater benthic algae. J. Appl. Phycol., 1, 39-52. , Keywords: heavy metal; photosynthesis; algae; tolerance , Strain(s): 71305329407408409410411412413414416433434454461465466467479481487489503504505506507509510515529530531532536537538539540620639640641642659660661662