The Microbial Culture Collection at the National Institute for Environmental Studies

Strain Data

Strain number NIES-40  
Phylum Cyanophyta   Class Cyanophyceae 
Scientific name Dolichospermum affine (Lemmermann) Wacklin, Hoffmann & Komárek  
Synonym -----  
Former name Anabaena affinis Lemmermann  
Common name Blue-green alga; Cyanobacteria  
Locality (Date of collection) Lake Kasumigaura Ibaraki Japan (1974-08-**)  
Habitat (Isolation source) Freshwater (Lake water)  
History < IAM (1983);  
Isolator (Date of isolation) Watanabe, Makoto M.; Ichimura, Terunobu (1974-08-**)  
Identified by Watanabe, Makoto M.  
State of strain Unialgal; Clonal; Non-axenic  
Preservation state
(State of preservation)
Culture condition
( Preculture condition )
Medium:  CT  
Temperature: 22 C
Light intensity: 20-25 µmol photons/m2/sec     L/D cycle: 10L:14D
Duration: 1 M  
Gene information 16S rRNA ( AY701541 AF247591 ) , pcB-pcA ( AF427973 AY702211 )
Cell size (min - max) 5 - 9 μm   Organization Filamentous 
Characteristics Cyanobacterial water bloom (aoko)  
Other strain no. Other collection strain no. : IAM M-168
Remarks Unstable 

54)   Baker, J. A., Entsch, B., McKay, D. B. 2003 The cyanobiont in an Azolla fern is neither Anabaena nor Nostoc. FEMS Mircobiol. Lett., 229, 43-47. , Keywords: Azolla fern; Cyanobacterial symbiont; Phylogenetic relationship , Strain(s): 4080 

64)   Beltran, E. C., Neilan, B. 2000 Geographical segregation of the neurotoxin-producing cyanobacterium Anabaena circinalis. Appl. Environ. Microbiol., 66, 4468-4474. , Strain(s): 192123404180 

334)   Hodoki, Y., Ohbayashi, K., Kobayashi, Y., Okuda, N., Nakano, S. 2012 Detection and identification of potentially toxic cyanobacteria: ubiquitous distribution of Microcystis aeruginosa and Cuspidothrix issatschenkoi in Japanese lakes. Harmful Algae, 16, 49-57. , Keywords: Anatoxin-a; Automated rRNA intergenic spacer analysis (ARISA); Cuspidothrix issatschenkoi; Cyanotoxin; Microcystin; Microcystis aeruginosa , Strain(s): 304077102111230512843905928932937991164516691698172919502116 

385)   Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 (in Japanese without English title). , Strain(s): 19212223242728303132333436373839404144454647484950122123127131144152173175179180181182183185198200203206217226227241266267268271295309310334338379436448450455527 

759)   Li, R., Watanabe, M., Watanabe, M. M. 2000 Taxonomic studies of planktic species of Anabaena based on morphological characteristics in cultured strains. Hydrobiologia, 438, 117-138. , Keywords: Anabaena; cyanobacteria; identification; morphology; planktic; taxonomy , Strain(s): 40737476777879802631652165316671672167316771695 

760)   Li, R., Yokota, A., Sugiyama, J., Watanabe, M., Hiroki, M., Watanabe, M. M. 1998 Chemotaxonomy of planktonic cyanobacteria based on non-polar and 3-hydroxy fatty acid composition. Phycol. Res., 46, 21-28. , Keywords: 3-hydroxy fatty acids; chemotaxonomy; fatty acid; non-polar fatty acids; planktonic cyanobacteria , Strain(s): 2740447678798081878998102104111112204205207298604810821825826 

904)   Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. , Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection , Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

1043)   Neilan, B. A., Jacobs, D., Goodman, A. E. 1995 Genetic diversity and phylogeny of toxic cyanobacteria determined by DNA polymorphisms within the phycocyanin locus. Appl. Environ. Microbiol., 61, 3875-3883. , Strain(s): 244041447376788081878998102107111112204207298 

1759)   Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title). , Strain(s): 1819212426293139404144454650737475767780818788899091939598991001011201221311351371441512042072132572633794152219223522392579 

1862)   Yamamoto, Y., Suzuki, K. 1990 Distribution and algal-lysing activity of fruiting myxobacteria in Lake Suwa. J. Phycol., 26, 457-462. , Keywords: algal-lysing activity; cyanobacteria; fruiting myxobacteria , Strain(s): 19404499102112