The Microbial Culture Collection at the National Institute for Environmental Studies

Strain Data

Strain number NIES-44  
Phylum Cyanophyta   Class Cyanophyceae 
Scientific name Microcystis aeruginosa (Kützing) Lemmermann  
Synonym Microcystis ichthyoblabe Kützing; Microcystis novacekii (Komárek) Compère; Microcystis viridis (A.Brown) Lemmermann; Microcystis wesenbergii Komárek  
Former name Microcystis aeruginosa (Kützing) Lemmermann f. aeruginosa  
Common name Blue-green alga; Cyanobacteria  
Locality (Date of collection) Lake Kasumigaura Ibaraki Japan (1974-08-**)  
Habitat (Isolation source) Freshwater  
History < IAM (1983);  
Isolator (Date of isolation) Watanabe, Makoto M. (1974-08-**)  
Identified by Watanabe, Makoto M.  
State of strain Unialgal; Clonal; Axenic  
Preservation state
(State of preservation)
Culture condition
( Preculture condition )
Medium:  CB  
Temperature: 25 C
Light intensity: 20-30 µmol photons/m2/sec     L/D cycle: 10L:14D
Duration: 20 D  
Gene information Whole-genome ( BBPA00000000 ) , 16S-23S-ITS ( AB015361 AB015387 AB254437 ) , ftsZ ( AB324850 ) , glnA ( AB324929 ) , gltX ( AB325008 ) , gyrB ( AB325087 ) , pgi ( AB325166 ) , recA ( AB325245 ) , tpi ( AB325324 )
Cell size (min - max) 6 - 8 μm   Organization  
Characteristics Cyanobacterial water bloom (aoko) ; ST1 (Tanabe, Y. et al. 2007); Genome decoded strain (Okano, K. et al. 2015)  
Other strain no. Other collection strain no. : IAM M-176 (=M-228)
Remarks Cryopreserved 

27)   Ando, T., Nagase, H., Eguchi, K., Hirooka, T., Nakamura, T., Miyamoto, K., Hirata, K. 2007 A novel method using cyanobacteria for ecotoxicity test of veterinary antimicrobial agents. Environ. Toxicol. Chem., 26, 601-606. , Keywords: Antimicrobial agent; Cyanobacterium; Ecotoxicity test; Algal toxicity , Strain(s): 192344107 

47)   Asada, Y., Kawamura, S., Ho, K.-K. 1987 Hydrogenase from the unicellular cyano-bacterium, Microcystis aeruginosa. Phytochemistry, 26, 637-640. , Keywords: Microcystis aeruginosa; cyanobacterium; hydrogenase; purification; properties , Strain(s): 44 

50)   Asai, R., Ootani, K., Nomura, Y., Nakamura, C., Ikebukuro, K., Arikawa, Y., Miyake, J., Karube, I. 2003 PCR-based ribosomal DNA detection technique for microalga (Heterosigma carterae) causing red tide and its application to a biosensor using labeled probe. Mar. Biotechnol., 5, 417-423. , Keywords: small subunit rDNA; Heterosigma carterae; fluorescence polarization; asymmetric polymerase chain reaction; surface plasmon resonance , Strain(s): 144 

93)   Choi, K., Imai, A., Kim, B., Matsushige, K. 2001 Properties of dissolved organic carbon (DOC) released by three species of blue-green algae. Korean J. Limnol., 34, 20-29. , Keywords: extracellular organic carbon; fractionation; blue-green algae; growth phases , Strain(s): 4474204 

133)   Dwi, S., Hirata, K., Asada, Y., Miyamoto, K. 2001 Utilization of cyanobacterial biomass from water bloom for bioproduction of lactic acid. World J. Microbiol. Biotech., 17, 259-264. , Keywords: cyanobacterium; CO2 supplementation; lactic acid; Lactobacillus agilis; L. amylovorus; L. ruminis; starch; water bloom , Strain(s): 2344 

249)   Hanazato, T. 1991 Interrelations between Microcystis and Cladocera in the highly eutrophic Lake Kasumigaura, Japan. Int. Revue ges. Hydrobiol., 76, 21-36. , Keywords: Microcystis; Cladocera; toxicity; food chain; Lake Kasumigaura , Strain(s): 44298299 

317)   Hirata, K., Phunchindawan, M., Tukamoto, J., Goda, S., Miyamoto, K. 1996 Cryopreservation of microalgae using encapsulation-dehydration. CryoLetters, 17, 321-328. , Keywords: Dunaliella tertiolecta; microalgae; cryopreservation; encapsulation-dehydration , Strain(s): 8192224273244242375396422437 

325)   Hirooka, T., Akiyama, Y., Tsuji, N., Nakamura, T., Nagase, H., Hirata, K., Miyamoto, K. 2003 Removal of hazardous phenols by microalgae under photoautotrophic conditions. J. Biosci. Bioeng., 95, 200-203. , Keywords: green algae; cyanobacteria; nitrophenol; chlorophenol; bisphenol A; photoautotrophic conditions , Strain(s): 192344422437 

385)   Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 (in Japanese without English title). , Strain(s): 19212223242728303132333436373839404144454647484950122123127131144152173175179180181182183185198200203206217226227241266267268271295309310334338379436448450455527 

396)   Ichimura, T., Watanabe, M. M. 1977 An axenic clone of Microcystis aeruginosa Kutz. emend. Elenkin from Lake Kasumigaura. Bull. Jpn. Soc. Phycol., 25, 177-181. , Strain(s): 44 

498)   Jang, M. H., Ha, K., Lucas, M. C., Joo, G. J., Takamura, N. 2004 Changes in microcystin production by Microcystis aeruginosa exposed to phytoplanktivorous and omnivorous fish. Aquat. Toxicol., 68, 51-59. , Keywords: Microcystis aeruginosa; microcystin; fish-induced defence; chemical cues; Hypophthalmichthys molitrix; Carassius gibelio langsdorfi , Strain(s): 448899 

503)   Jang, M-H., Ha, K., Joo, G-J., Takamura, N. 2003 Toxin production of cyanobacteria is increased by exposure to zooplankton. Freshw. Biol., 48, 1540-1550. , Keywords: info-chemicals; microcystin production; Microcystis aeruginosa; zooplankton; zooplankton culture media filtrate , Strain(s): 44878899 

512)   Jung, S. W., Kim, B.-H., Katano, T., Kong, D.-S., Han, M.-S. 2008 Pseudomonas flurescens HYK0210-SK09 offers species-specific biological control of winter algal blooms caused by freshwater diatom Stephanodiscus hantzschii. J. Appl. Microbiol., 105, 186-195. , Keywords: algicidal bacterium; algicidal mechanism; biological control; indoor mesocosm; Pseudomonas fluorescens; Stephanodiscus hantzschii , Strain(s): 1944298 

551)   Kang, B.-H., Tan, S., Ho, K.-K. 1988 Isolation of water-soluble cytochromes from cyanobacteria by adsorption chromatography. Archi. Biochem. Biophys., 263, 387-393. , Strain(s): 44 

552)   Kang, C. B. H., Ho. K.-K. 1991 Characteri-zation of a soluble inorganic pyrophosphatase from Microcystis aeruginosa. Archi. Biochem. Biophys., 289, 281-288. , Strain(s): 44 

555)   Kang, Y.-H., Park, C.-S., Han, M.-S. 2012 Pseudomonas aeruginosa UCBPP-PA14 a useful bacterium capable of lysing Microcystis aeruginosa cells and degrading microcystins. J. Appl. Phycol., 24, 1517-1525. , Keywords: Microcystis aeruginosa; Microcystins; Algicidal bacteria; Pseudomonas aeruginosa , Strain(s): 4494298 

570)   Kasai, F., Takamura, N., Hatakeyama, S. 1993 Effects of simetryne on growth of various freshwater algal taxa. Environ. Pollut., 79, 77-83. , Strain(s): 35447377102137151230231255275280298 

573)   Kataoka, T., Homma, T., Nakano, S., Hodoki, Y., Ohbayashi, K., Kondo, R. 2013 PCR primers for selective detection of intra-species variations in the bloom-forming cyanobacterium, Microcystis. Harmful Algae, 23, 46-54. , Keywords: Microcystis aeruginosa; Quantitative PCR; 16S-23S rDNA ITS fegion; Selective detection; Intra-species variation , Strain(s): 4487909810110210310711111229860410621070 

607)   Kaya, K., Shiraishi, F., Uchida, H., Sano, T. 2011 A novel retinoic acid analogue, 7-hydroxy retinoic acid, isolated from cyanobacteria. Biochim. Biophys. Acta, 1810, 414-419. , Keywords: 7-hydroxy retinoic acid; RAR agonistic activity; cyanobacteria; UV stability , Strain(s): 444546512610931 

760)   Li, R., Yokota, A., Sugiyama, J., Watanabe, M., Hiroki, M., Watanabe, M. M. 1998 Chemotaxonomy of planktonic cyanobacteria based on non-polar and 3-hydroxy fatty acid composition. Phycol. Res., 46, 21-28. , Keywords: 3-hydroxy fatty acids; chemotaxonomy; fatty acid; non-polar fatty acids; planktonic cyanobacteria , Strain(s): 2740447678798081878998102104111112204205207298604810821825826 

783)   Lyra, C., Suomalainen, S., Gugger, M., Vezie, C., Sundman, P., Paulin, L., Sivonen, K. 2001 Molecular characterization of planktic cyanobacteria of Anabaena, Aphanizomenon, Microcystis and Planktothrix genera. Int. J. Syst. Evol. Microbiol., 51, 13-526. , Keywords: cyanobacteria; 16S rRNA gene; RFLP; sequencing; REP; ERIC , Strain(s): 41447379818998102104 

904)   Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. , Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection , Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

940)   Nagai, T., Imai, A., Matsushige, K., Fukushima, T. 2005 Limiting nutrients on the growth of bloom-forming cyanobacteria with special focus on iron speciation. Verh. Internat. Verein. Limnol., 29, 949-952. , Keywords: limiting nutrient; algal growth potential test; speciation; iron , Strain(s): 44204 

941)   Nagai, T., Imai, A., Matsushige, K., Fukushima, T. 2006 Effect of iron complexation with dissolved organic matter on the growth of cyanobacteria in a eutrophic lake. Aquat. Microb. Ecol., 44, 231-239. , Keywords: dissolved organic matter; DOM; cyanobacteria; iron; organic complexation , Strain(s): 44204 

942)   Nagai, T., Imai, A., Matsushige, K., Fukushima, T. 2007 Growth characteristics and growth modeling of Microcystis aeruginosa and Planktothrix agardhii under iron limitation. Limnology, 8, 261-270. , Keywords: iron; growth rate; uptake rate; model simulation; cyanobacteria , Strain(s): 44204 

952)   Nagase, H., Inthorn, D., Oda, A., Nishimura, J., Kajiwara, Y., Park, M.-O., Hirata, K., Miyamoto, K. 2005 Improvement of selective removal of heavy metals in cyanobacteria by NaOH treatment. J. Biosci. Bioeng., 99, 372-377. , Keywords: cyanobacteria; cadmium; zinc; copper; lead; hard water , Strain(s): 2344 

1043)   Neilan, B. A., Jacobs, D., Goodman, A. E. 1995 Genetic diversity and phylogeny of toxic cyanobacteria determined by DNA polymorphisms within the phycocyanin locus. Appl. Environ. Microbiol., 61, 3875-3883. , Strain(s): 244041447376788081878998102107111112204207298 

1053)   Ninomiya, K., Ogino, C., Kawabata, S., Kitamura, K., Maki, T., Hasegawa, H., Shimizu, N. 2013 Ultrasonic inactivation of Microcystis aeruginosa in the presence of TiO2 particles. J. Biosci. Bioeng., 116, 214-218. , Keywords: Ultrasaound; Titanium dioxide; Disinfection; Microcystis aeruginosa; Hydroxyl radicals , Strain(s): 44 

1169)   Ohkouchi, Y., Tajima, S., Nomura, M., Itoh, S. 2015 Inflammatory responses and potencies of various lipopolysaccharides from bacteria and cyanobacteria in aquatic environments and water supply systems. Toxicon, 97, 23-31. , Keywords: Lipopolysaccharide (LPS); Purification; Endotoxin; Indigenous bacteria/cyanobacteria; Human monocytic cells; Cytokine secretion , Strain(s): 44946 

1213)   Okano, K., Miyata, N., Ozaki, Y. 2015 Whole genome sequence of the non-microcystin-producing Microcystis aeruginosa strain NIES-44. GenomeA, 3, e00135-15 (article ID). , Strain(s): 44 

1219)   Okubo, Y., Inoue, T., Yokoto, K. 2012 Estimating bioavailability of soil particulate phosphorus toMicrocystis aeruginosa. J. Appl. Phycol., 24, 1503-1507. , Keywords: Particulate phosphorus; Bioavailable phosphorus; Microcystis aeruginosa; Cyanobacteria; Sequential phosphorus extraction , Strain(s): 44 

1260)   Otsuka, S., Suda, S., Li, R., Watanabe, M., Oyaizu, H., Matsumoto, S., Watanabe, M. M. 1999 Phylogenetic relationships between toxic and non-toxic strains of the genus Microcystis based on 16S to 23S internal transcribed spacer sequence. FEMS Mircobiol. Lett., 172, 15-21. , Keywords: cyanobacteria; Microcystis; internal transcribed spacer; phylogeny; microcystin , Strain(s): 44104111105410551058106710681072108510861090109110921105111511171143 

1263)   Oyama, Y., Matsushita, B., Fukushima, T., Chen, J., Nagai, T., Imai, A. 2010 Testing the spectral decomposition algorithm (SDA) for different phytoplankton species by a simulation based on tank experiments. Int. J. Remote Sens., 31, 1605-1623. , Strain(s): 4475204803 

1313)   Saito, K., Matsumoto, M., Sekine, T., Murakoshi, I. 1989 Inhibitory substances from Myriophyllum brasiliense of growth of blue-green algae. J. Nat. Prod., 52, 1221-1226. , Strain(s): 4473 

1492)   Soma, Y., Imaizumi, T., Yagi, K., Kasuga, S. 1993 Estimation of algal succession in lake water using HPLC analysis of pigments. Can. J. Fish. Aquat. Sci., 50, 1142-1146. , Strain(s): 44738188104111233234333351 

1529)   Sun, L.W., Jiang, W.J., Sato, H., Kawachi,M., Lu, X.W. 2016 Rapid classification and identification of Microcystis aeruginosa strains using MALDI-TOF MS and polygenetic analysis. PLoS One, 11, e0156275 (article ID). , Strain(s): 44878889901001011041051071091111124786048439011043105310581061106810691070107210761077108510941099110111041105111311151126113411351144115111571170121112131215121612171218122212241230123212331239 

1530)   Susilaningsih, D. 2007 Accelerating of pink pigment excretion from cyanobacterium Oscillatoria by Co-Cultivation with Anabaena. HHAYATI J. Biosci., 14, 18-22. , Keywords: Oscillatoria; Anabaena variabilis; biosurfactant; cyanobacterium; pink pigment , Strain(s): 192344422 

1544)   Tachibana, H. 1984 Fundamental study on algal growth potential. Proc. Environ. Sanit. Eng. Res., 20, 53-60 (in Japanese). , Strain(s): 44 

1545)   Tachibana, H., Moriguchi, A., Inoue, T., Imaoka, T. 1986 Fundamental study on algal growth potential. II. Utilization of particulate nutrients by algae in river waters. Proc. Environ. Sanit. Eng. Res., 22, 151-161 (in Japanese). , Strain(s): 442170 

1607)   Tan, S., Ho, K.-K. 1989 Purification of an acidic plastocyanin from Microcystis aeruginosa. Biochim. Biophys. Acta, 973, 111-117. , Keywords: plastocyanin; cytochrome c-553; absorption chromatography; cyanobacteria; photosynthesis , Strain(s): 44 

1610)   Tanabe, Y., Kasai, F., Watanabe, M. M. 2007 Multilocus sequence typing (MLST) reveals high genetic diversity and clonal population structure of the toxic cyanobacterium Microcystis aeruginosa. Microbiology, 153, 3695-3703. , Strain(s): 44878889909198991001011021031041051061071081091101111122982994786048439019331050105110521053105410551056105710581059106010611062106310641065106610671068106910701072107510761077108510901091109210931094109510961097109811001101110211031104110511061107110811131114111511221123112411251126112711281129113011311132113311341135113611371138113911401141114211431144114511461147114811491150115111521153115511561157115911601161116211631164117011711172120912111213121412151216121712181219122012211222122312241225122612271228122912301231123212331234123512361237123812391240124112421243124412451251125212531254 

1613)   Tanabe, Y., Sano, T., Kasai, F., Watanabe, M. M. 2009b Recombination, cryptic clades and neutral molecular divergence of the microcystin synthetase (mcy) genes of toxic cyanobacterium Microcystis aeruginosa. BMC Evol. Biol., 9, 115 (article ID). , Strain(s): 44878889909198991001011021031041051061071081091101111122982994786048439011050105110521053105410551056105710581059106010611062106310641065106610671068106910701072107510761077108510901091109210931094109510961097109811001101110211031104110511061107110811131114111511221123112411251126112711281129113011311132113311341135113611371138113911401141114211431144114511461147114811491150115111521153115511561157115911601161116211631164117011711172120912111213121412151216121712181219122012211222122312241225122612271228122912301231123212331234123512361237123812391240124112421243124412451251125212531254246524662467246924702471247224732474247524762477247824792561 

1614)   Tanabe, Y., Watanabe, M. M. 2011 Local expansion of a panmictic lineage of water bloom-forming cyanobacterium Microcystis aeruginosa. PLoS One, 6, e17085 (article ID). , Strain(s): 448788899091989910010110210310410510610710810911011111229829947860484390110501051105210531054105510561057105810591060106110621063106410651066106710681069107010721075107610771085109010911092109310941095109610971098110011011102110311041105110611071108111311141115112211231124112511261127112811291130113111321133113411351136113711381139114011411142114311441145114611471148114911501151115211531155115611571159116011611162116311641170117111721173117411751176117711791180118111831184118511861209121112131214121512161217121812191220122112221223122412251226122712281229123012311232123312341235123612371238123912401241124212431244124512511252125312542088208920902091246524662467246824692470247124722473247424752476247724782479248024812482248324842485248624872488248924902491249224932494249524962546254725482549255025512552255325542555255625572558255925602561259125922593259425952596259725982599260026012602260326042605260626072608260926102611261226132617261826192620262126222623262426252626262726282629 

1627)   Tezuka, M., Satou, Y., Nakamura, M. 2005 The effect of phosphorus in the fine-grained soil on the eutrophication of closed water area. J. JSCE [Doboku-kôgaku Ronbunshû], 804 (VII-37), 113-123 (in Japanese with English summary). , Keywords: eutrophication; fine-grained soil; phosphorus , Strain(s): 44 

1710)   Wada, Y., Yokotera, H., Yamazaki, K., Toyonaga, K. 2004 Study on the multiply capability of phytoplankton at the artificial repetition on blight and dark condition. Advance in River Engineering, Vol.10, 465-470 (in Japanese with English summary). , Keywords: destratification system; multiply capability; blight and dark condition; Microcystis; algal growth potential; record of light shield , Strain(s): 44 

1759)   Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title). , Strain(s): 1819212426293139404144454650737475767780818788899091939598991001011201221311351371441512042072132572633794152219223522392579 

1771)   Watanabe, M. M., Sawaguchi, T. 1995 Cryopreservation of a water-bloom forming cyanobacterium, Microcystis aeruginosa f. aeruginosa. Phycol. Res., 43, 111-116. , Keywords: cryopreservation; DMSO; Microcystis aeruginosa f. aeruginosa; rapid cooling; two step cooling , Strain(s): 4490 

1862)   Yamamoto, Y., Suzuki, K. 1990 Distribution and algal-lysing activity of fruiting myxobacteria in Lake Suwa. J. Phycol., 26, 457-462. , Keywords: algal-lysing activity; cyanobacteria; fruiting myxobacteria , Strain(s): 19404499102112 

1904)   Yoshida, T., Takashima, Y., Tomaru Y., Shirai, Y., Takao, Y., Hiroishi, S., Nagasaki, K. 2006 Isolation and characterization of a cyanophage infecting the toxic cyanobacterium Microcystis aeruginosa. Appl. Environ. Microbiol., 72, 1239-1247. , Strain(s): 44879098102104111112298604 

1978)   Shinohara, A., Imai, A., Komatsu, K., Matsushige, K., Nara, F. 2008 Application of HPLC-PAD to highly sensitive analysis of dissolved carbohydrates and theit composition in lake water and extracellular organic matter derived from algae. J. Jpn. Soc. Wat. Environ., 31, 447-454 (in Japanese with English summary). , Keywords: carbohydrates; DOM; HPLC-PAD; lake water; algal culture , Strain(s): 4474204