Strain number NIES-293  
Phylum Heterokontophyta  
Class Raphidophyceae  
Scientific name Heterosigma akashiwo (Hada) Hada ex Y.Hara & Chihara  
Synonym -----  
Former name  
Common name  
Locality (Date of collection) Onagawa Bay Miyagi Japan (1984-08-28)  
Latitude / Longitude  
Habitat (Isolation source) Marine (Seawater)  
History < Suda, Shoichiro  
Isolator (Date of isolation) Suda, Shoichiro (1984-09-01)  
Identified by Suda, Shoichiro  
State of strain Subculture; Unialgal; Clonal; Axenic[2016 Jul]  
Culture condition
(Preculture condition)
Medium:  f/2  
Temperature:  20 C
Light intensity:  32-40 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  20 D  
Gene information Plastid DNA ( EU168190 ) , 18S-ITS1-5.8S-ITS2-28S ( DQ470658 ) , CO1 ( AB334512 ) , rbcL ( AB334439 )  
Cell size (min - max)  
Organization Unicellular; Flagellate 
Characteristics Red tide ; Genome decoded strain (Cattolico, R. A. et al. 2008)  
Other strain no. Other strain no. : 8280G21-1  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1" 
Movie  
Reference
Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29.
Keywords: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae
Strain(s): 1891415177111514522322523323426527427527627727827928028128228429332332433033334434534634734835035337237738839139540740840941341441746146246648753454855355655755855956056258758858959060360562162262369569669769869970070170270370470570670770870971071171271371471571674176576676780583799710011002100310041005100610071009101110161017104410451046104713021303132413271330133913401349135313701375137613791383138413851386138713911392139313951398139914001401169917001730181318151816182618271831186218631864186518741963196419652142214321442145214721482300233123322351236323642365237025062533253425352536253725902633266826892690269126932694269626972716271727182720272227232725272627292730273127322770277127722773283928402841284228432844285928782890 

Saijo, H., Tsuruta, K., Kusumoto, N., Ashitani, T., Takahashi, K. 2013 Growth inhibition activities of Sugi bark components against Heterosigma akashiwo. J. Wood Sci., 59, 238-242.
Keywords: Sugi bark; Extract; Inhibition activity; Red tide plankton; Heterosigma akashiwo
Strain(s): 293 

Yang, EC., Boo, GH., Kim, HJ., Cho, SM., Boo, SM., Andersen, R. A., Yoon, HS. 2012 Supermatrix data highlight the phylogenetic relationships of photosynthetic Stramenopiles. Protist, 163, 217-231.
Keywords: Heterokont algae; molecular phylogenetic analysis; multigene phylogeny; reduced flagellar clade; stramenochromes
Strain(s): 29362271618631864 
PubMed: 22001261
DOI: 10.1016/j.protis.2011.08.001

Park, B. S., Baek, S. H., Ki, J.-S., Cattolico, R. A., Han, M.-S. 2012 Assessment of EvaGreen-based quantitative real-time PCR assay for enumeration of the microalgae Heterosigma and Chattonella (Raphidophyceae). J. Appl. Phycol., 24, 1555-1567.
Keywords: Raphidophyceae; Quantitative real-time PCR; EvaGreen; Field application
Strain(s): 293 

Demura, M., Noël, M.-H., Kasai, F., Watanabe, M. M., Kawachi, M. 2009 Taxonomic revision of Chattonella antiqua, C. marina and C. ovata (Raphidophyceae) based on their morphological characteristics and genetic diversity. Phycologia, 48, 518-535.
Keywords: Chattonella antiqua; Chattonella marina; Chattonella ovata; COI; COX1; Genetic diversity; ITS; Microsatellite; Morphology; Phylogeny; Population; Raphidophyceae; rbcL; Speciation; Taxonomy
Strain(s): 1235691014838485113114116118145146161293557561603671716849238323842385238623872388238923912392239323942395239623972398239924002401240224032404240524062407 
DOI: 10.2216/08-98.1

Cattolico, R. A., Jacobs, M. A., Zhou, Y., Chang, J., Duplessis, M., Lybrand, T., McKay, J., Ong, H. C., Sims, E., Rocap, G. 2008 Chloroplast genome sequencing analysis of Heterosigma akashiwo CCAM452 (West Atlantic) and NIES293 (West Pacific) strains. BMC Genomics, 9, 211-230.
Strain(s): 293 

Ki, J.-S., Han, M.-S. 2007 Nuclear rDNA and chloroplast rbcL, rbcS and IGS sequence data, and their implications from the Japanese, Korean, and North American harmful algae, Heterosigma akashiwo (Raphidophyceae). Environ. Res., 103, 299-304.
Keywords: Heterosigma akashiwo; blooms; nuclear rDNA; rbcS; IGS
Strain(s): 293 

Hosoi-Tanabe, S., Sako, Y. 2005 Species-specific detection and quantification of toxic marine Dinoflagellates Alexandrium tamarense and A. catenella by reai-time PCR assay. Mar. Biotechnol., 7, 506-514.
Keywords: toxic dinoflagellate; — Alexandrium; paralytic shellfish poisoning (PSP); real-time PCR; species-specific identification; quantification
Strain(s): 1712293331559603670 

Takeda, F., Sakamaki, T., Xu, K., Chiba, N., Nishimura, O., Sudo, R. 2003 Effect of potential allelochemicals extracted from Sargassum horneri on the growth of red tide microalgae. J. Environ. Syst. and Eng., JSCE, VII-29, 25-32.
Keywords: Sargassum horneri; red tide microalgae; growth inhibition; allelochemicals
Strain(s): 116293 

Connell, L., Cattolico, R. A. 1996 Fragile algae: axenic culture of field-collected samples of Heterosigma carterae. Mar. Biol., 125, 421-426.
Strain(s): 293 

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