株番号 NIES-4118  
門名 Dinophyta  
綱名 Dinophyceae 
学名 Gambierdiscus scabrosus T.Nishimura, Shinya Sato & M.Adachi  
シノニム  
以前使われていた種名  
一般名 渦鞭毛藻  
地名(採集年月) 日本 高知県 大月町 柏島、後浜 (2007-09-22)  
緯度/経度 32.772778 / 132.625278 
生息環境(分離源) 海域 (海藻)  
分離後の履歴 ← 中村正利; 足立真佐雄  
分離者(分離年月) 中村正利 (2007-09-**)  
同定者 西村朋宏; 佐藤晋也; 足立真佐雄  
株の状態 継代培養; 非無菌; 単藻; クローン  
培養条件
(前培養条件)
培地名:  MNK  
温度: 20 ℃
光強度: 10 µmol photons/m2/sec,  明暗周期: 10L:14D
継代培養周期: 45 D  
遺伝子情報 18S rRNA ( AB764244 AB764245 AB764246 AB764247 AB764248 ) , 28S rRNA ( AB765908 AB765909 )  
特性 有毒 ; 付着性 ; Authentic株 (Nishimura et al. 2014)  
サイズ (min - max) 49 - 83 μm  
体制 単細胞性 
その他の株番号 その他の株番号:KW070922_1  
注意事項 有毒  
動画  
文献
Pisapia, F., Holland, W. C., Hardison, D. R., e Litake, R. W., Fraga, S., Nishimura, T., Adachi, M., Nguyen-Ngoc, L., Séchet, V., Amzil, Z., Herrenknecht, C., Hess, P. 2017 Toxicity screening of 13 Gambierdiscus strains using neuro-2a and erythrocyte lysis bioassays. Harmful Algae, 63, 173-183.
キーワード: Ciguatera fish poisoning; Gambierdiscus; Ciguatoxins; Maitotoxins; Neuro-2a assay; Erythrocyte lysis assay
Strain(s): 4118 
PubMed: 28366392
DOI: 10.1016/j.hal.2017.02.005

Pisapia, F., Sibat, .M., Herrenknecht, C., Lhaut, K., Gaiani, G., Ferron, P., Fessard, V., Fraga, S., Nascimento, S. M., Litaker, R. W., Holland, W. C., Roullier, C., Hess, P. 2017 Maitotoxin-4, a novel MTX analog produced by Gambierdiscus excentricus. Mar. Drugs, 15, 220 (article ID).
キーワード: Gambierdiscus excentricus; maitotoxin-4; bioguided fractionation; size-exclusion chromatography (LH-20); neuro-2a (N2a) assay; LC-HRMS/MS (Q-Tof 6550); LC-LRMS/MS (API4000 QTrap)
Strain(s): 41184119 
PubMed: 28696398
DOI: 10.3390/md15070220

Nishimura, T., Hariganeya, N., Tawong, W., Sakanari, H., Yamaguchi, H., Adachi, M. 2016 Quantitative PCR assay for detection and enumeration of ciguatera-causing dinoflagellate Gambierdiscus spp. (Gonyaulacales) in coastal areas of Japan. Harmful Algae, 52, 11-22.
キーワード: Ciguatera fish poisoning; Dinoflagellate; Gambierdiscus; Japan: Quantitative PCR assay
Strain(s): 41184119 
DOI: 10.1016/j.hal.2015.11.018

Yoshimatsu, T., Tie, C., Yamaguchi, H., Funaki, H., Honma, C., Tanaka, K., Adachi, M. 2016 The effects of light intensity on the growth of Japanese Gambierdiscus spp. (Dinophyceae). Harmful Algae, 60, 107-115.
キーワード: Ciguatera; Gambierdiscus; Growth; Ligh
Strain(s): 41184119 
DOI: 10.1016/j.hal.2016.10.009

Nishimura, T., Sato, S., Tawong, W., Sakanari, H., Yamaguchi, H., Adachi, M. 2014 Morphology of Gambierdiscus scabrosus sp. nov. (Gonyaulacales): a new epiphytic toxic dinoflagellate from coastal areas of Japan. J. Phycol., 50, 506-514.
キーワード: ciguatera fish poisoning; epiphytic; dinoflagellate; Gambierdiscus scabrosus; Japan; LM; morphology; SEM; taxonomy
Strain(s): 4118 
PubMed: 26988323
DOI: 10.1111/jpy.12175

Yoshimatsu, T., Yamaguchi, H., Iwamoto, H., Nishimura, T., Adachi, M. 2014 Effects of temperature, salinity and their interaction on growth of Japanese Gambierdiscus spp. (Dinophyceae). Harmful Algae, 35, 29-37.
キーワード: Ciguatera; Japanese Gambierdiscus; Growth; Temperature; Salinity
Strain(s): 41184119 
DOI: 10.1016/j.hal.2014.03.007

Nishimura, T., Sato, S., Tawong, W., Sakanari, H., Uehara, K., Shah, M.M., Suda, S., Yasumoto, T., Taira, Y., Yamaguchi, H., Adachi, M. 2013 Genetic diversity and distribution of the ciguatera-causing dinoflagellate Gambierdiscus spp. (Dinophyceae) in coastal areas of Japan. PLoS One, 8, e60882 (article ID).
Strain(s): 41184119 
PubMed: 23593339
DOI: 10.1371/journal.pone.0060882

Hariganeya, N., Tanimoto, Y., Yamaguchi, H., Nishimura, T., Tawong, W., Sakanari, H., Yoshimatsu, T., Sato, S., Preston, C.M., Adachi, M. 2013 Quantitative PCR method for enumeration of cells of cryptic species of the toxic marine dinoflagellate Ostreopsis spp. in coastal waters of Japan. PLoS One, 8, e57627 (article ID).
Strain(s): 4118 
PubMed: 23593102
DOI: 10.1371/journal.pone.0057627

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