Strain number NIES-262  
Phylum Streptophyta  
Class Charophyceae  
Scientific name Closterium peracerosum-strigosum-littorale complex  
Synonym  
Former name  
Common name Desmid  
Locality (Date of collection) Piuthan Nepal (1965-10-14)  
Latitude / Longitude  
Habitat (Isolation source) Freshwater (Reservoir sediment)  
History < Ichimura, Terunobu  
Isolator (Date of isolation) Ichimura, Terunobu  
Identified by Ichimura, Terunobu  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  C  
Temperature:  15 (20) C
Light intensity:  7 (10-17) µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M (1 M)  
Gene information 18S rRNA ( LC037438 )  
Cell size (min - max)  
Organization Unicellular 
Characteristics Heterothallic ; Mating type (−) ; Mating group I A  
Other strain no. Other collection strain no. : IAM C-441(=C-577)
Other strain no. : N-13-4  
Remarks  
Movie  
Reference
Tsuchikane, Y., Kobayashi, H., Kato, M., Watanabe, J., Wu, J. T., Sekimoto, H. 2018 Identification of a new mating group and reproductive isolation in the Closterium peracerosum–strigosum–littorale complex. J. Plant Res., 131, 735–746.
Keywords: Alga; Closterium; Mating group; Reproductive isolation; Sex pheromone; Speciation
Strain(s): 5354556465666768261262 
PubMed: 29948484
DOI: 10.1007/s10265-018-1043-8

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Watanabe, M. M. 1977 Biosystematics in Closterium of sexual unicellular green algae and Calothrix and Spirulina of asexual filamentous blue-green algae, with special reference to the analyses of natural populations. Thesis D. Sci., Hokkaido University, 114 pp., 28 tables, 54 figs.
Strain(s): 3945465152535455565758596061626364656667686970261262266268 

Ichimura, T. 1973 The life cycle and its control in some species of Closterium, with special reference to the biological species problems. Thesis D. Sci., University of Tokyo, 69 pp., 11 tables, 40 figs.
Strain(s): 666768124125127174175176177179180181182183185198202262337338450451 

Ichimura, T. 1973 Culture of microalgae (6). Iden, 27, 97-101 (in Japanese).
Strain(s): 66173175179180181183198262338450 

Ichimura, T. 1971 Sexual cell division and conjugation-papilla formation in sexual reproduction of Closterium strigosum. In Proceedings of the Seventh International Seaweed Symposium, University of Tokyo Press, Tokyo, p. 208-214.
Strain(s): 66262 

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