株番号 NIES-229  
門名 Streptophyta  
綱名 Charophyceae 
学名 Closterium ehrenbergii Meneghini ex Ralfs  
シノニム  
以前使われていた種名  
一般名 接合藻 ; ミカヅキモ ; オオミカヅキモ  
地名(採集年月) 日本 神奈川県 海老名市 (1975-12-04)  
緯度/経度 35.437621 / 139.393061 
生息環境(分離源) 淡水 (水田の土)  
分離後の履歴 ← 市村輝宜  
分離者(分離年月) 市村輝宜 (-----)  
同定者 市村輝宜  
株の状態 継代培養; 無菌[2013 Jan]; 単藻; クローン  
培養条件
(前培養条件)
培地名:  C  
温度: 20 (20) ℃
光強度: 4-8 (13-21) µmol photons/m2/sec,  明暗周期: 10L:14D
継代培養周期: 3 M (1 M)  
遺伝子情報 18S rRNA ( AY148821 ) , 5.8S-ITS2-28S ( AY148898 ) , scdip-1mRNA ( AB066448 )  
特性 雌雄異株 ; 交配型 (−) ; 交配群B ; NIES-228と交配  
サイズ (min - max) 75 - 500 μm  
体制 単細胞性 
その他の株番号 その他の株番号:KK-33-6  
注意事項  
動画  
文献
Tsuchikane, Y., Ito, M., Sekimoto, H. 2008 Reproductive isolation by sex pheromones in the Closterium peracerosum-strigosum-littorale complex (Zygnematales, Charophyceae). J. Phycol., 44, 1197-1203.
キーワード: Closterium; conjugation; pheromone; reproductive isolation; sexual reproduction
Strain(s): 53545859646567686970229261 

Ciniglia, C., Cascone, C., Giudice, R. L., Pinto, G., Pollio, A. 2005 Application of methods for assessing the geno- and cytotoxicity of Triclosan to C. ehrenbergii. J. Hazard. Mater., 122, 227-232.
キーワード: Closterium ehrenbergii; Triclosan; sexual reproduction; vegetative reproduction; comet assay
Strain(s): 228229 

Fukumoto, R., Fujii, T., Sekimoto, H. 2003 Cloning of a cDNA encoding a sexual cell division-inducing pheromone from a unicellular green alga Closterium ehrenbergii. J. Phycol., 39, 931-936.
キーワード: Closterium; conjugation; pheromone; sexual reproduction; RACE-PCR
Strain(s): 228229 

Denboh, T., Ichimura, T., Hendrayanti, D., Coleman, A. W. 2003 Closterium moniliferum-ehrenbergii (Charophyceae, Chlorophyta) species complex viewed from the 1506 Group I intron and ITS2 of nuclear rDNA. J. Phycol., 39, 960-977.
キーワード: biological species; Closterium; group I introns; ITS2; mating group; molecular phylogeny; speciation
Strain(s): 172173174182183228229 

Fukumoto, R., Dohmae, N., Takio, K., Satoh, S., Fujii, T., Sekimoto, H. 2002 Purification and characterization of a pheromone that induces sexual cell division in the unicellular green alga Closterium ehrenbergii. Plant Physiol. Biochem., 40, 183-188.
キーワード: Closterium ehrenbergii; pheromone; sexual reproduction
Strain(s): 228229 

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
キーワード: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Ogawa, S., Hirota, K. 2000 Disintegration of chloroplasts during zygote maturation in Closterium ehrenbergii (Zygnematales, Chlorophyta). Int. J. Plant Sci., 161, 609-614.
キーワード: chloroplast; Closterium; conjugation; disintegration of chloroplast; Zygnematales; zygote maturation
Strain(s): 228229 

Sekimoto, H. 2000 Intercellular communication during sexual reproduction of Closterium (Conjugatophyceae). J. Plant Res., 113, 343-352.
キーワード: chemotaxis; Closterium; conjugation; mating; sex pheromone; sexual reproduction
Strain(s): 6465228229 

Ogawa, S., Iokawa, Y., Oba, T., Watanabe, T. 1999 Application of low-vacuum scanning electron microscope (LV SEM) to the classes for plankton observation. Bull. Joetsu Univ. Educ., 19, 99-109 (in Japanese with English summary).
キーワード: freeze-drying method; low-vacuum scanning electron microscope (LV SEM); plankton; scanning electron microscope (SEM)
Strain(s): 229 

Fukumoto, R., Fujii, T., Sekimoto, H. 1998 A newly identified chemotactic sexual pheromone from Closterium ehrenbergii. Sex. Plant Reprod., 11, 81-85.
キーワード: Closterium ehrenbergii; chemoattractant; conjugation; sexual pheromone; protoplast-release inducing protein
Strain(s): 228229 

Fukumoto, R., Fujii, T., Sekimoto, H. 1997 Detection and evaluation of a novel sexual pheromone that induces sexual cell division of Closterium ehrenbergii (Chlorophyta). J. Phycol., 33, 441-445.
キーワード: Chlorophyta; Closterium ehrenbergii; conjugation; gametogenesis; gamete; pheromone; protoplast-release-inducing protein; sexual cell division
Strain(s): 228229 

Hino, S. 1991 An examination of algal bioassay method for agricultural chemicals and heavy metals in a water environment. Hokkaido Kankyô Kagaku Kenkyû Sentâ Syohô, 18, 41-49 (in Japanese with English summary).
Strain(s): 228229 

Kasai, F., Ichimura, T. 1990 Temperature optima of three closely related mating groups of the Closterium ehrenbergii (Chlorophyta) species complex. Phycologia, 29, 396-402.
Strain(s): 228229 

Ichimura, T., Kasai, F. 1987 Time-lapse analyses of sexual isolation between two closely related mating groups of the Closterium ehrenbergii species complex (Chlorophyta). J. Phycol., 23, 523-534.
キーワード: Closterium ehrenbergii; desmids; mating group; multiple-choice mating; no-choice mating; sexual interactions; sexual isolation; time-lapse photomicrography
Strain(s): 228229 

Kasai, F., Ichimura, T. 1986 Morphological variabilities of three closely related mating groups of Closterium ehrenbergii Meneghini (Chlorophyta). J. Phycol., 22, 158-168.
キーワード: cell shape and size; clonal culture; Closterium ehrenbergii; mating group; morphological variability; statistical analysis
Strain(s): 171228229 

Ichimura, T., Kasai, F. 1984 Post-zygotic isolation between allopatric mating groups of Closterium ehrenbergii Meneghini (Conjugato-phyceae). Phycologia, 23, 77-85.
Strain(s): 228229 

Ichimura, T., Kasai, F. 1984 Time lapse analyses of sexual reproduction in Closterium ehrenbergii (Conjugatophyceae). J. Phycol., 20, 258-265.
キーワード: Closterium ehrenbergii; conjugation; desmids; mating type; sexual reproduction
Strain(s): 228229 

Ichimura, T. 1983 Hybrid inviability and predominant survival of mating type minus progeny in laboratory crosses between two closely related mating groups of Closterium ehrenbergii. Evolution, 37, 252-260.
Strain(s): 228229 

Ichimura, T. 1981 Mating types and reproductive isolation in Closterium ehrenbergii Meneghini. Bot. Mag. Tokyo, 94, 325-334.
キーワード: biosystematic study; Closterium ehrenbergii; mating group; mating type; reproductive isolation
Strain(s): 228229 
DOI: 10.1007/BF02493393

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