The Microbial Culture Collection at the National Institute for Environmental Studies

Strain Data

A big image opens when the thumbnail is clicked.

Strain number NIES-228  
Phylum Streptophyta   Class Charophyceae 
Scientific name Closterium ehrenbergii Meneghini ex Ralfs  
Synonym -----  
Former name  
Common name Desmid  
Locality (Date of collection) Ebina Ebina Kanagawa Japan (1975-12-04)  
Habitat (Isolation source) Freshwater (Paddy soil)  
History < Ichimura, Terunobu  
Isolator (Date of isolation) Ichimura, Terunobu (-----)  
Identified by Ichimura, Terunobu  
State of strain Unialgal; Clonal; Axenic[2013 Jan]  
Preservation state
(State of preservation)
Subculture  
Culture condition
( Preculture condition )
Medium:  C  
Temperature: 20(25) C
Light intensity: 8-15(60-70) µmol photons/m2/sec     L/D cycle: 12L:12D
Duration: 3 M(1 M)  
Gene information 18S rRNA ( AY148818 ) , 5.8S-ITS2-28S ( AY148895 ) , cdip-1 mRNA ( AB066448 )
Cell size (min - max) - 150 μm   Organization Unicellular; Non-flagellate 
Characteristics Heterothallic; Mating type(+); Mating group B; Crosses with NIES-229  
Other strain no. Other strain no. : KK-33-1  
Remarks  

Reference
67)   Ciniglia, C., Cascone, C., Giudice, R. L., Pinto, G., Pollio, A. 2005 Application of methods for assessing the geno- and cytotoxicity of Triclosan to C. ehrenbergii. J. Hazard. Mat., 122, 227-232. , Keywords: Closterium ehrenbergii; Triclosan; sexual reproduction; vegetative reproduction; comet assay , Strain(s): 228229 

82)   Denboh, T., Ichimura, T., Hendrayanti, D., Coleman, A. W. 2003 Closterium moniliferum-ehrenbergii (Charophyceae, Chlorophyta) species complex viewed from the 1506 Group I intron and ITS2 of nuclear rDNA. J. Phycol., 39, 960-977. , Keywords: molecular phylogeny; taxonomy; Volvocaceae; Volvulina compacta , Strain(s): 172173174182183228229 

134)   Fukumoto, R., Dohmae, N., Takio, K., Satoh, S., Fujii, T., Sekimoto, H. 2002 Purification and characterization of a pheromone that induces sexual cell division in the unicellular green alga Closterium ehrenbergii. Plant Physiol. Biochem., 40, 183-188. , Keywords: Closterium ehrenbergii; pheromone; sexual reproduction , Strain(s): 228229 

135)   Fukumoto, R., Fujii, T., Sekimoto, H. 1997 Detection and evaluation of a novel sexual pheromone that induces sexual cell division of Closterium ehrenbergii (Chlorophyta). J. Phycol., 33, 441-445. , Keywords: Chlorophyta; Closterium ehrenbergii; conjugation; gametogenesis; gamete; pheromone; protoplast-release-inducing protein; sexual cell division , Strain(s): 228229 

136)   Fukumoto, R., Fujii, T., Sekimoto, H. 1998 A newly identified chemotactic sexual pheromone from Closterium ehrenbergii. Sex. Plant Reprod., 11, 81-85. , Keywords: Closterium ehrenbergii; chemoattractant; conjugation; sexual pheromone; protoplast-release inducing protein , Strain(s): 228229 

137)   Fukumoto, R., Fujii, T., Sekimoto, H. 2003 Cloning of a cDNA encoding a sexual cell division-inducing pheromone from a unicellular green alga Closterium ehrenbergii. J. Phycol., 39, 931-936. , Keywords: Closterium; conjugation; pheromone; sexual reproduction; RACE-PCR , Strain(s): 228229 

221)   Hino, S. 1991 An examination of algal bioassay method for agricultural chemicals and heavy metals in a water environment. Hokkaido Kankyô Kagaku Kenkyû Sentâ Syohô, 18, 41-49 (in Japanese with English summary). , Strain(s): 228229 

274)   Ichimura, T. 1981 Mating types and reproductive isolation in Closterium ehrenbergii Meneghini. Bot. Mag. Tokyo, 94, 325-334. , Keywords: biosystematic study; Closterium ehrenbergii; mating group; mating type; reproductive isolation , Strain(s): 228229 

275)   Ichimura, T. 1983 Hybrid inviability and predominant survival of mating type minus progeny in laboratory crosses between two closely related mating groups of Closterium ehrenbergii. Evolution, 37, 252-260. , Strain(s): 228229 

278)   Ichimura, T., Kasai, F. 1984 Post-zygotic isolation between allopatric mating groups of Closterium ehrenbergii Meneghini (Conjugato-phyceae). Phycologia, 23, 77-85. , Strain(s): 228229 

279)   Ichimura, T., Kasai, F. 1984 Time lapse analyses of sexual reproduction in Closterium ehrenbergii (Conjugatophyceae). J. Phycol., 20, 258-265. , Keywords: Closterium ehrenbergii; conjugation; desmids; mating type; sexual reproduction , Strain(s): 228229 

281)   Ichimura, T., Kasai, F. 1987 Time-lapse analyses of sexual isolation between two closely related mating groups of the Closterium ehrenbergii species complex (Chlorophyta). J. Phycol., 23, 523-534. , Keywords: Closterium ehrenbergii; desmids; mating group; multiple-choice mating; no-choice mating; sexual interactions; sexual isolation; time-lapse photomicrography , Strain(s): 228229 

304)   Imamura, N., Motoike, I., Shimada, N., Nishikori, M., Morisaki, H., Fukami, H. 2001 An efficient screening approach for anti-Microcystis compounds based on knowledge of aquatic microbial ecosystem. J. Antibiotics, 54, 582-587. , Strain(s): 102228 

414)   Kasai, F., Ichimura, T. 1986 Morphological variabilities of three closely related mating groups of Closterium ehrenbergii Meneghini (Chlorophyta). J. Phycol., 22, 158-168. , Keywords: cell shape and size; clonal culture; Closterium ehrenbergii; mating group; morphological variability; statistical analysis , Strain(s): 171228229 

415)   Kasai, F., Ichimura, T. 1990 Temperature optima of three closely related mating groups of the Closterium ehrenbergii (Chlorophyta) species complex. Phycologia, 29, 396-402. , Strain(s): 228229 

459)   Kim, Y.-S., Oyaizu, H., Matsumoto, S, Watanabe, M. M., Nozaki, H. 1994 Chloroplast small-subunit ribosomal RNA gene sequence from Chlamydomonas parkeae (Chlorophyta) : molecular phylogeny of a green alga with a peculiar pigment composition. Eur. J. Phycol., 29, 213-217. , Keywords: Chlamydomonas parkeae; Chlorophyta; chloroplast small-subunit (SSU) rRNA; phylogeny; pigment composition , Strain(s): 228254440468666 

653)   Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. , Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection , Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195196197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

853)   Ogawa, S., Hirota, K. 2000 Disintegration of chloroplasts during zygote maturation in Closterium ehrenbergii (Zygnematales, Chlorophyta). Int. J. Plant Sci., 161, 609-614. , Keywords: chloroplast; Closterium; conjugation; disintegration of chloroplast; Zygnematales; zygote maturation , Strain(s): 228229 

1016)   Sasaki, G., Katoh, K., Hirose, N., Suga, H., Kuma, K., Miyata, T., Su, Z. H. 2007 Multiple receptor-like kinase cDNAs from liverwort Marchantia polymorpha and two charophycean green algae, Closterium ehrenbergii and Nitella axillaris: Extensive gene duplications and gene shufflings in the early evolution of streptophytes. Gene, 401(1-2), 135-144. , Keywords: conjugate alga; stonewort; gene family; divergence; phylogenetic tree; molecular evolution , Strain(s): 228 

1044)   Sekimoto, H. 2000 Intercellular communication during sexual reproduction of Closterium (Conjugatophyceae). J. Plant Res., 113, 343-352. , Keywords: chemotaxis; Closterium; conjugation; mating; sex pheromone; sexual reproduction , Strain(s): 6465228229 

1089)   Shinozaki, A., Sato, N., Hayashi, Y. 2009 Peroxisomal targeting signals in green algae. Protoplasma, 235, 57-66. , Keywords: Closterium; green algae; peroxisomal targeting signal (PTS); peroxisome; transport , Strain(s): 228