Strain Data

Strain number	NIES-545
Phylum	Chlorophyta	Class	Chlorophyceae
Scientific name	Volvulina steinii Playfair
Synonym	-----
Former name
Common name	Green alga
Locality (Date of collection)	Hayama Hayama Kanagawa Japan (1980-12-**)
Habitat (Isolation source)	Freshwater (Paddy soil)
History	< Nozaki, Hisayoshi
Isolator (Date of isolation)	Nozaki, Hisayoshi (1981-01-07)
Identified by	Nozaki, Hisayoshi
State of strain	Unialgal; Clonal; Axenic[2013 Jan]
Preservation state (State of preservation)	Subculture
Culture condition ( Preculture condition )	Medium: VTAC Temperature: 20 C Light intensity: 15-27 µmol photons/m²/sec L/D cycle: 12L:12D Duration: 1 M
Gene information	atpB ( AB044713 ) , psaA ( AB044221 AB044222 ) , psaB ( AB044448 ) , psbC ( AB044500 ) , rbcL ( AB044159 )
Cell size (min - max)		Organization	Colonial; Flagellate
Characteristics	Heterothallic; Mating type(−); Crosses with NIES-546
Other strain no.	Other strain no. : 1107-5 (-)
Remarks

Reference
16) Ando, T., Otsuka, S., Nishiyama, M., Sendo, K., Watanabe, M. M., Matsumoto, S. 2003 Toxic effects of dichloromethane and trichloroethlene on the growth of planktonic green algae, Chlorella vulgaris NIES227, Selenastrum capricornutum NIES35, and Volvulina steinii NIES545. Microb. Environ., 18, 43-46. , Keywords: algal growth potential (AGP); bioindicator; dichloromethane; organic solvents; trichloroethlene , Strain(s): 35, 227, 545 632) Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. , Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection , Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 124, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 148, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 262, 263, 266, 267, 268, 271, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 311, 312, 313, 329, 334, 336, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729 731) Nakazawa, A., Nishii, I. 2012 Amidic and acetonic cryoprotectants improve cryopreservation of volvocine green algae. CryoLetters, 33, 201-212. , Keywords: cryopreservation; dimethylformamide; hydroxyacetone; Volvocaceae; Volvox; Chlamydomonas , Strain(s): 418, 468, 545, 564, 574, 717, 721, 728, 729, 730, 731, 877, 1331, 1361, 1710 760) Nozaki, H. 1982 Morphology and reproduction of Japanese Volvulina steinii (Chlorophyta, Volvocales). J. Jpn. Bot., 57, 105-113, pls. 3-4. , Strain(s): 545, 546, 896, 897, 898 783) Nozaki, H., Hara, Y., Kasaki, H. 1987 Light and electron microscopy of pyrenoids and species delimitation in Volvulina (Chlorophyta, Volvocaceae). J. Phycol., 23, 359-364. , Keywords: Chlorophyta; culture age; pyrenoid; pyrenoid development; taxonomy; ultrastructure; Volvocaceae; Volvulina pringsheimii; Volvulina steinii , Strain(s): 545, 896, 897, 898 804) Nozaki, H., Misawa, K., Kajita, T., Kato, M., Nohara, S., Watanabe, M. M. 2000 Origin and evolution of the colonial Volvocales (Chlorophyceae) as inferred from multiple, chloroplast gene sequences. Mol. Phylog. Evol., 17, 256-268. , Strain(s): 418, 456, 460, 474, 541, 545, 564, 566, 569, 571, 572, 574, 577, 582, 653, 654, 666, 720, 721, 722, 724, 725, 727, 728, 730, 731, 732, 734, 735, 736, 737, 851, 854, 872, 882, 884, 886, 887, 888, 889, 890, 893, 895, 896, 898