The Microbial Culture Collection at the National Institute for Environmental Studies

Strain Data

Strain number NIES-3774  
Phylum Chlorophyta   Class Chlorophyceae 
Scientific name Pyrobotrys squarrosa (Korshikov) Korshikov  
Synonym Chlamydobotrys squarrosa Korshikov 1928; Uva squarrosa (Korshikov) Fott 1967; Pyrobotrys squarrosa (Korshikov) Huber-Pestalozzi in Fott 1967  
Former name  
Common name Green alga  
Locality (Date of collection) Akiragi, Hagi Yamaguchi Japan (2013-01-08)  
Habitat (Isolation source) Freshwater (Sediment)  
History < Sugasawa, Mizuho  
Isolator (Date of isolation) Sugasawa, Mizuho (2014-04-08)  
Identified by Sugasawa, Mizuho  
State of strain Unialgal; Clonal  
Preservation state
(State of preservation)
Culture condition
( Preculture condition )
Medium:  AF-6  
Temperature: 20 C
Light intensity: 24-35 µmol photons/m2/sec     L/D cycle: 10L:14D
Duration: 21 D  
Gene information
Cell size (min - max)   Organization Colonial; Flagellate 
Characteristics Mixotrophic; Osmotrophic; Phototaxis; Microaerophilic  
Other strain no. Other strain no. : YS21  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1"; Culturing under microaerophilic condition 

1522)   Sugasawa, M., Matsuzaki, R., Kawafune, K., Takahashi, T., Kawachi, M., Krientiz, L., Nozaki, H. 2015 Taxonomic study of Pyrobotrys (Spondylomoraceae, Chlorophyceae) based on comparative morphological and molecular analyses of culture strains established using novel methods. Cytologia, 80, 513-524. , Keywords: Molecular phylogeny; Morphology; Pyrobotrys; Pyrobotrys elongata; Spondylomoraceae; Stigma; Taxonomy; Ultrastructure , Strain(s): 3768376937703771377237733774