Strain number NIES-3766  
Phylum Amoebozoa  
Class Discosea  
Scientific name Vannella simplex Wohlfarth-Bottermann  
Synonym  
Former name  
Common name  
Locality (Date of collection) Westveld Pond Sint-Amandsberg, Gent Belgium (2008-07-04)  
Latitude / Longitude 51.06805555 / 3.77383333 
Habitat (Isolation source) Freshwater (Pond Water)  
History < Van Wichelen, Jeroen < Vanormelingen, Pieter  
Isolator (Date of isolation) Vanormelingen, Pieter (2008-07-25)  
Identified by Van Wichelen, Jeroen  
State of strain Subculture; Mixed (NIES-3730 as food)  
Culture condition
(Preculture condition)
Medium:  WC  
Temperature:  20 C
Light intensity:  7-8 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S rRNA ( KP719197 )  
Cell size (min - max)  
Organization  
Characteristics predator of water bloom–forming species ; Phagotrophic ; Planktonic ; Epiphytic ; Resting spore-forming  
Other strain no. Other strain no. : A17WVB  
Remarks  
Movie https://www.youtube.com/watch?v=lGHrTVsWfIA&list=PLFTJlLsZcVbiJY3ygYAPKeVOfDdOh9UEW&index=18  
Reference
Van Wichelen, J., D'hondt, S., Claeys, M., Vyverman, W., Berney, C., Bass, D., Vanormelingen, P. 2016 A hotspot of amoebae diversity: 8 new naked amoebae associated with the planktonic bloom-forming cyanobacterium Microcystis. Acta Protozool., 55, 61-87.
Keywords: Naked amoebae; Microcystis; morphology; 18S rDNA; harmful algal blooms; grazing; Europe
Strain(s): 372237233725372637283729373037663767 
DOI: 10.4467/16890027AP.16.007.4942

Van Wichelen, J., Van Gremberghe, I., Vanormelingen, P., Debeer, A-E., Leporcq, B., Menzel, D., Codd, G. A., Descy, J-P., Vyverman, W. 2010 Strong effects of amoebae grazing on the biomass and genetic structure of a Microcystis bloom (Cyanobacteria). Environ. Microbiol., 12, 2797-2813.
Strain(s): 3722372637303766 
PubMed: 20545742
DOI: 10.1111/j.1462-2920.2010.02249.x

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