The Microbial Culture Collection at the National Institute for Environmental Studies

Strain Data

Strain number NIES-3753  
Phylum Chlorophyta   Class Chlorophyceae 
Scientific name Chloromonas krienitzii Matsuzaki & Nozaki  
Former name  
Common name Green alga; Snow alga  
Locality (Date of collection) Yamagata Prefectural Nature Conservation Park, Mt. Gassan Nishikawa Yamagata Japan (2013-06-30)  
Habitat (Isolation source) Terrestrial (Snow)  
History < Matsuzaki, Ryo  
Isolator (Date of isolation) Matsuzaki, Ryo (2014-01-24)  
Identified by Matsuzaki, Ryo; Nozaki, Hisayoshi  
State of strain Unialgal; Clonal  
Preservation state
(State of preservation)
Culture condition
( Preculture condition )
Medium:  AF-6  
Temperature: 5 C
Light intensity: 10 µmol photons/m2/sec     L/D cycle: 10L:14D
Duration: 3 M  
Gene information 18S rRNA ( LC012712 ) , 26S rRNA ( LC060474 ) , ITS2 ( LC012756 ) , atpB ( LC012720 ) , psaB ( LC012728 ) , rbcL ( LC012740 )
Cell size (min - max) 5 - 10 μm   Organization Unicellular 
Characteristics ; Authentic strain (Matsuzaki, R. et al. 2015); Cryophilic  
Other strain no. Other strain no. : GsCl-54  

826)   Matsuzaki, R., Kawai-Toyooka, H., Hara, Y., Nozaki, H. 2015 Revisiting the taxonomic significance of aplanozygote morphologies of two “cosmopolitan” snow species of the genus Chloromonas (Volvocales, Chlorophyceae). Phycologia, 54, 491–502. , Keywords: Chloromonas brevispina; Chloromonas krienitzii sp. nov.; Chloromonas nivalis; Molecular phylogeny; Morphology; Snow algae; Ultrastructure , Strain(s): 2379238037523753