Strain Data

Strain number	NIES-226
Phylum	Chlorophyta	Class	Chlorophyceae
Scientific name	Graesiella emersonii (Shihira et Krauss) Nozaki et al.
Synonym	Chlorella emersonii Shihira et Krauss; Chlorella fusca Shihira et Krauss var. vacuolata Shihira et Krauss; Graesiella vacuolata (Shihira et Krauss) Kalina et Puncochárová
Former name	Chlorella pyrenoidosa Chick
Common name	Green alga
Locality (Date of collection)	Japan (-----)
Habitat (Isolation source)
History	< IAM (1983);
Isolator (Date of isolation)	(-----)
Identified by	Nozaki, Hisayoshi et al. (Reidentify)
State of strain	Unialgal; Clonal; Axenic
Preservation state (State of preservation)	Cryopreservation
Culture condition ( Preculture condition )	Medium: C (agar) Temperature: 20(25) C Light intensity: 4-10(70-80) µmol photons/m²/sec L/D cycle: 12L:12D Duration: 3 M(14 D)
Gene information
Cell size (min - max)	5 - 9 μm	Organization	Unicellular; Non-flagellate
Characteristics
Other strain no.	Other collection strain no. : IAM C-28
Remarks	Cryopreserved

Reference
230) Hirokawa, T., Takeda, H. 1980 Automatic analysing system for the liquid chromatography. J. Gen. Edu. Dept., Niigata Univ., 10, 97-106. , Strain(s): 226, 2170 273) Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 (in Japanese without English title). , Strain(s): 19, 21, 22, 23, 24, 27, 28, 30, 31, 32, 33, 34, 36, 37, 38, 39, 40, 41, 44, 45, 46, 47, 48, 49, 50, 122, 123, 127, 131, 144, 152, 173, 175, 179, 180, 181, 182, 183, 185, 198, 200, 203, 206, 217, 226, 227, 241, 266, 267, 268, 271, 295, 309, 310, 334, 338, 379, 436, 448, 450, 455, 527 557) Maruyama, I., Ando, Y., Maeda, T., Hirayama, K. 1989 Uptake of vitamin B₁₂ by various strains of unicellular algae Chlorella. Nippon Suisan Gakkaishi, 55, 1785-1790. , Strain(s): 48, 226, 687, 2157, 2169, 2175 559) Maruyama, K. 1977 Classification of Chlorella strain by infrared absorption spectra of cell samples. Bot. Mag. Tokyo, 90, 67-77. , Strain(s): 226, 227, 685, 687, 2151, 2163, 2170, 2175 560) Maruyama, K. 1977 Classification of Chlorella strains by cell appearance and group sera. Bot. Mag. Tokyo, 90, 57-66. , Strain(s): 226, 227, 685, 2151, 2163, 2170, 2175 586) Mihara, S., Hase, E. 1976 A short note on the effects of 6-methyl purine on the revolution of the cell cycle of Chlorella pyrenoidosa in synchronous culture. Plant Cell Physiol., 17, 403-406. , Strain(s): 226 635) Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. , Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection , Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 124, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 148, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 262, 263, 266, 267, 268, 271, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 311, 312, 313, 329, 334, 336, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729 738) Naohara, T., Ishikawa, H. 1975 Assay of the activity of herbicides using Chlorella. Zassô-Kenkyû, 20, 82-86 (in Japanese with English summary). , Strain(s): 226 795) Nozaki, H., Katagiri, M., Nakagawa, M., Aizawa, K., Watanabe, M. M. 1995 Taxonomic re-examination of the two strains labeled Chlorella in the Microbial Culture Collection at the National Institute for Environmental Studies (NIES-Collection). Microbiol. Cult. Coll., 11, 11-18. , Keywords: Chlorella; Graesiella emersonii comb. nov.; green algae; morphology; taxonomy , Strain(s): 226, 227, 686, 687, 688, 689, 690 1136) Takeda, H. 1988 Classification of Chlorella strains by cell wall sugar composition. Phytochemistry, 27, 3823-3826. , Keywords: Chlorella ellipsoidea; C. fusca; C. kessleri; C. saccharophila; C. sorokiniana; C. vulgaris; Chlorophyta; anisotropy; cell wall sugar composition; chemotaxonomy; Ruthenium Red staining , Strain(s): 226, 2150, 2160, 2170 1249) Watanabe, A. 1960 List of algal strains in collection at the Institute of Applied Microbiology, University of Tokyo. J. Gen. Appl. Microbiol., 6, 283-292. , Strain(s): 19, 21, 22, 23, 24, 25, 26, 28, 29, 37, 47, 49, 226, 227 1312) Watanabe, Y., Ohmura, N., Saiki, H. 1992 Microbial CO₂ fixation. 2. Isolation and determination of cultural characteristics of Chlorella strains which function under CO₂ enriched atmosphere. CRIEPI Kenkyû-Hôkoku, U92014, 1-21 (in Japanese with English summary). , Keywords: climate change; CO2; CO2 fixation; microalgae; Chlorella , Strain(s): 27, 45, 92, 94, 122, 158, 204, 226, 227 1399) Yoshizako, F., Nishimura, A., Chubachi, M. 1992 Microbial reduction of cyclohexanone by Chlorella pyrenoidosa Chick. J. Ferment. Bioeng., 74, 395-397. , Strain(s): 226 1400) Yoshizako, F., Nishimura, A., Chubachi, M. 1994 Identification of algal transformation products from alicyclic ketones. J. Ferment. Bioeng., 77, 144-147. , Strain(s): 226 1401) Yoshizako, F., Nishimura, A., Chubachi, M., Horii, T., Ueno, T. 1991 Bioconversion of cyclohexaneacetic acid to monohydroxycyclo-hexaneacetic acids by Chlorella pyrenoidosa Chick. J. Ferment. Bioeng., 72, 343-346. , Strain(s): 226, 227 1402) Yoshizako, F., Ogino, M., Nishimura, A., Chubachi, M., Horii, T. 1995 Biotransformation of cyclic β-keto esters by Chlorella pyrenoidosa Chick. J. Ferment. Bioeng., 79, 141-145. , Keywords: Chlorella pyrenoidosa Chick; cyclic β-keto esters; biotransformation; alkoxycarbolyl group elimination; carbonyl group reduction , Strain(s): 226