The Microbial Culture Collection at the National Institute for Environmental Studies

Strain Data

Strain number NIES-3749  
Phylum Chlorophyta   Class Chlorophyceae 
Scientific name Pascherina tetras (Korshikov) P.C.Silva  
Synonym Pascheriella tetras Korshikov 1928; Archiv fur Protistenkunde:232  
Former name  
Common name Green alga  
Locality (Date of collection) Kawagoe Saitama Japan (2011-02-24)  
Habitat (Isolation source) Freshwater (Water)  
History < Matsuzaki, Ryo  
Isolator (Date of isolation) Matsuzaki, Ryo (2011-02-24)  
Identified by Sugasawa, Mizuho; Matsuzaki, Ryo; Nozaki, Hisayoshi  
State of strain Unialgal; Clonal  
Preservation state
(State of preservation)
Culture condition
( Preculture condition )
Medium:  AF-6  
Temperature: 20 C
Light intensity: 60 µmol photons/m2/sec     L/D cycle: 10L:14D
Duration: 1 M  
Gene information 18S rRNA ( LC053638 ) , rbcL ( LC053639 ) , psaB ( LC053640 )
Cell size (min - max)   Organization Colonial 
Characteristics Phototaxis  
Other strain no. Other strain no. : Isa28  

1521)   Sugasawa, M., Matsuzaki, R., Arakaki, Y., Nozaki, H. 2015 Morphology and phylogenetic position of a rare four-celled green alga, Pascherina tetras (Volvocales, Chlorophyceae), based on cultured material. Phycologia, 54, 342-348. , Keywords: Culture strain; Molecular phylogeny; Morphology; Multicellularity; Spondylomoraceae; Ultrastructure , Strain(s): 37493750