Strain number NIES-3669  
Phylum Chlorophyta  
Class Chloropicophyceae  
Scientific name Chloroparvula pacifica Lopes dos Santos, Noël & Eikrem  
Synonym  
Former name  
Common name  
Locality (Date of collection) the western North Pacific Ocean Japan (2012-10-03)  
Latitude / Longitude 42.1634 / 145.0674 
Habitat (Isolation source) Marine (Seawater)  
History < Noël, Mary-Hélène  
Isolator (Date of isolation) Noël, Mary-Hélène (2013-02-08)  
Identified by Identified at NIES by DNA analysis  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  ESM  
Temperature:  20 C
Light intensity:  24 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  25 D  
Gene information Mitochondrial DNA ( MK086011 ) , Plastid DNA ( MK085995 ) , 16S rRNA ( KU843560 ) , 18S rRNA ( KU843574 ) , ITS1-5.8S-ITS2-28S ( MF077486 )  
Cell size (min - max)  
Organization Unicellular 
Characteristics Genome decoded strain (Turmel et al. 2019) ; Authentic strain (Lopes dos Santos et al. 2017) ; Clade VIIB1  
Other strain no. Other collection strain no. : RCC 4656
Other strain no. : MH 509  
Remarks  
Movie  
Reference
Turmel, M., Lopes Dos Santos, A., Otis, C., Sergerie, R., Lemieux, C. 2019 Tracing the evolution of the plastome and mitogenome in the Chloropicophyceae uncovered convergent tRNA gene losses and a variant plastid genetic code. Genome Biol. Evol., 11, 1275–1292.
Keywords: prasinophyte green algae; Picocystis salinarum; chloroplast genome; mitochondrial genome; phylogenomic analyses; AUA codon reassignment
Strain(s): 275527583669 
PubMed: 30937436
DOI: 10.1093/gbe/evz074

Lopes dos Santos, A., Pollina, T., Gourvil, P., Corre, E., Marie, D., Garrido, J. L., Rodríguez, F., Noël, M.-H., Vaulot, D., Eikrem, W. 2017 Chloropicophyceae, a new class of picophytoplanktonic prasinophytes. Sci Rep, 7, 14019 (article ID).
Strain(s): 27552756275836673668366936703671 
PubMed: 10.3775/jie.96.352
DOI: 10.1038/s41598-017-12412-5

Lopes dos Santos, A., Gourvil, P., Tragin, M., Noël, M.-H., Decelle, J., Romac, S., Vaulot, D. 2016 Diversity and oceanic distribution of prasinophytes clade VII, the dominant group of green algae in oceanic waters. ISME J., 11, 512-528.
Strain(s): 2755275627583668366936703671 
PubMed: 27779617
DOI: 10.1038/ismej.2016.120

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