The Microbial Culture Collection at the National Institute for Environmental Studies

Strain Data

Strain number NIES-3637  
Phylum Dinophyta   Class Dinophyceae 
Scientific name Coolia malayensis Leaw, P.-T.Lim & Usup  
Former name  
Common name Dinoflagellate  
Locality (Date of collection) Teniya, Nago Okinawa Japan (2013-08-**)  
Habitat (Isolation source) Marine (Seaweed)  
History < Wakeman, Kevin  
Isolator (Date of isolation) Wakeman, Kevin (2013-08-**)  
Identified by Wakeman, Kevin  
State of strain Unialgal; Non-axenic  
Preservation state
(State of preservation)
Culture condition
( Preculture condition )
Medium:  f/2  
Temperature: 20 C
Light intensity: 17 µmol photons/m2/sec     L/D cycle: 10L:14D
Duration: 1 M  
Gene information 18S-ITS1-5.8S-ITS2 ( KJ933433 ) , 18S-ITS1-5.8S-ITS2-28S ( KJ933432 KJ933434 KJ933435 ) , 28S rRNA ( KJ933436 KJ933437 KJ933438 KJ933439 )
Cell size (min - max)   Organization Unicellular 
Characteristics Toxic , Benthic; Epiphytic  
Other strain no. Other strain no. : C1  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1" 

1713)   Wakeman, K. C., Yamaguchi, A., Roy, M. C., Jenke-Kodama, H. 2015 Morphology, phylogeny and novel chemical compounds from Coolia malayensis (Dinophyceae) from Okinawa, Japan. Harmful Algae, 44, 8-19. , Keywords: Coolia malayensis; Cooliatoxin; High-resolution MS; Morphology; NanoLC/MS; Molecular phylogeny , Strain(s): 3637