株番号 NIES-3600  
門名 Cyanophyta  
綱名 Cyanophyceae 
学名 Trichodesmium sp.  
シノニム  
以前使われていた種名  
一般名 藍藻 ; シアノバクテリア  
地名(採集年月) 日本 黒潮海流、伊豆半島沖 (1983-10-13)  
緯度/経度  
生息環境(分離源) 海域 (海水)  
分離後の履歴 ← 大城香  
分離者(分離年月) 大城香 (1983-10-13)  
同定者 大城香  
株の状態 継代培養; 非無菌; 単藻; クローン  
培養条件
(前培養条件)
培地名:  改変AQUIL  
温度: 25 ℃
光強度: 20 µmol photons/m2/sec,  明暗周期: 10L:14D
継代培養周期: 20 D  
遺伝子情報 nifH ( L00688 )  
特性 窒素固定  
サイズ (min - max)  
体制 糸状 
その他の株番号 その他の株番号:NIBB1067  
注意事項 不安定; 輸送時の衝撃に弱い; 輸送時の温度変化に弱い  
動画  
文献
Ohki, K. 2008 Intercellular localization of nitrogenase non-heterocystous Cyanobacterium (Cyanophyte), Trichodesmium sp. NIBB 1067. J. Oceanogr., 64, 211-216.
キーワード: Trichodesmium spp.; nitrogenase; whole-cell immunolocalization; immunocytochemical detection; in situ hybridization; diazotrophic cyanobacteria; horseradish peroxidaseconjugated probe; 3,3'-diaminobenzidine
Strain(s): 3600 

Taniuchi, Y., Murakami, A., Ohki, K. 2008 Whole-cell immunocytochemical detection of nitrogenase in cyanobacteria: improved protocol for highly fluorescent cells. Aquat. Microb. Ecol., 51, 237-247.
キーワード: Diazotrophic unicellular cyanobacteria; Marine cyanobacteria; Immunocytochemical detection; Nitrogenase; Unicellular cyanobacteria
Strain(s): 3600 
DOI: 10.3354/ame01197

Mulhollannd, M. R., Ohki, K., Capone, D. G. 2001 Nutrient controls on nitrogen uptake and metabolism by natural populations and cultures of Trichodesmium (Cyanobacteria). J. Phycol., 37, 1001-1009.
キーワード: Glutamate; glutamine; glutamine synthetase; N2 fixation; N uptake; Trichodesmium
Strain(s): 3600 
DOI: 10.1046/j.1529-8817.2001.00080.x

Mulhollannd, M. R., Ohki, K., Capone, D. G. 1999 Nitrogen utilization and metabolism relative to pattern of N2 fixation in cultures of Trichodesmium NIBB1067. J. Phycol., 35, 977-988.
キーワード: amino acids; glutamate; glutamine; glutamine synthetase; N turnover; N2 fixation; NH4+ uptake; NO3-; Trichodesmium; urea
Strain(s): 3600 
DOI: 10.1046/j.1529-8817.1999.3550977.x

Mulholland, M.R., Shoemaker, C., Ohki, K., Capone, D.G. 1999 Utilization of combined forms of N in cultures and populations of Trichodesmium spp.. In Marine cyanobacteria and related organisms, Eds. Charpy, L., Larkum, Bulletin de l'Institut Oceanographique, Monaco, Special paper, 19, p. 279-286.
Strain(s): 3600 

Ohki, K. 1999 A possible role of temperate phage in the regulation of Trichodesmium biomass. In Marine cyanobacteria and related organisms, Eds. Charpy, L., Larkum, Bulletin de l'Institut Oceanographique, Monaco, Special paper, 19, p. 287-292.
Strain(s): 3600 

Ohki, K., Kumazawa, S., Yoe, T-W., Ho, K-K. 1998 Improvement of artificial medium for the culture of marine microalgae and isolation and characterization of marine cyanobactria(Cyanophyta) from Singapore. In Asian Network on Microbial Researches, Eds. Gadjah Mada University, Riken and Science and Technology Agency, Japan 147-154.
Strain(s): 3600 

Ohki, K., Zehr, J. P., Fujita, Y. 1992 Regulation of nitrogenase activity in relation to the light-dark regime in the filamentous non-heterocystous cyanobacterium Trichodesmium sp. NIBB 1067. J. Gen. Microbiol., 138, 2679-2685.
Strain(s): 3600 
DOI: 10.1099/00221287-138-12-2679

Ohki, K., Zehr, J. P., Fujita, Y. 1992 Trichodesmium: Establishment of culture and characteristics of N2-fixation. In Marine Pelagic Cyanobacteria: Trichodesmium and other Diazotrophs, Eds. by Carpenter, E.J., Capone, D.G., Rueter, J.G., Springer, Dordrecht, 362, p. 307-318.
Strain(s): 3600 
DOI: 10.1007/978-94-015-7977-3_20

Ohki, K., Fujita, Y. 1992 Light-dependent maintenance of active nitrogenase in the non-heterocystous cyanophyte Trichodesmium sp. NIBB10687. In Research in photosynthesis, Ed. Murata, N., Kluwer Academic Publishers, IV, p. 103-106.
Strain(s): 3600 

Ohki, K., Zehr, J. P., Falkowsk, P. G., Fujita, Y. 1991 Regulation of nitrogen-fixation by different nitrogen sources in the marine non-heterocystous cyanobacterium Trichodesmium sp. NIBB1067. Arch. Microbiol., 156, 335-337.
キーワード: Cyanobacterium; Trichodesmium; Nitrogen-fixation; Nitrogenase
Strain(s): 3600 
DOI: 10.1007/BF00248706

Zehr, J. P., Ohki, K., Fujita, Y. 1991 Arrangement of nitrogenase structural genes in an aerobic filamentous nonheterocystous cyanobacterium. J. Bacteriol., 173, 7055-7058.
Strain(s): 3600 
PubMed: 1938909
DOI: 10.1128/jb.173.21.7055-7058.1991

Zehr, J. P., Ohki, K., Fujita, Y., Landry, D. 1991 Unique modification of adenine in genomic DNA of the marine cyanobacterium Trichodesmium sp. strain NIBB 1067. J. Bacteriol., 173, 7059-7062.
Strain(s): 3600 
PubMed: 1657876
DOI: 10.1128/jb.173.21.7059-7062.1991

Ohki, K., Falkowski, P.G., Rueter, J. G., Fujita, Y. 1991 Experimental study of the marine cyanophyte Trichodesmium sp., a nitrogen-fixing phytoplankton in tropical and subtropical sea area. In Marine biology: Its accomplishment and future prospect, Eds. by Mauchline, J., Nemoto, T., Hokusen-Sha, p. 205-216.
Strain(s): 3600 

Rueter, J. G., Ohki, K., Fujita,Y. 1990 The effect of iron nutrition on photosynthesis and nitrogen fixation in cultures of Trichodesmium (cyanophyceae). J. Phycol., 26, 30-35.
Strain(s): 3600 
DOI: 10.1111/j.0022-3646.1990.00030.x

Ohki, K., Fujita, Y. 1988 Aerobic nitrogenase activity measured as acetylene reduction in the marine non-heterocystous cyanobacterium Trichodesmium spp. grown under artificial conditions. Mar. Biol., 98, 111-114.
Strain(s): 3600 
DOI: 10.1007/BF00392665

Ohki, K., Rueter, J. G., Fujita, Y. 1986 Cultures of the pelagic cyanophytes Trichodesmium erythraeum and T. thiebautii in synthetic medium. Mar. Biol., 91, 9-13.
Strain(s): 3600 
DOI: 10.1007/BF00397565

Ohki, K., Fujita, Y. 1982 Laboratory culture of the pelagic blue-green alga Trichodesmium thiebautii: conditions for unialgal culture. Mar. Ecol. Prog. Ser., 7, 185-190.
Strain(s): 3600 

Morel, F.M.M., Rueter, J.G., Anderson,D.M., Guillard, R.R. 1979 Aquil: a chemically defined phytoplankton culture medium for trace metal studies. J. Phycol., 15, 135-141.
Strain(s): 3600 
DOI: 10.1111/j.1529-8817.1979.tb02976.x

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