Strain number NIES-3576  
Phylum Heterokontophyta  
Class Bacillariophyceae  
Scientific name Nitzschia sp.  
Synonym  
Former name  
Common name Pennate diatom  
Locality (Date of collection) Miyara River Ishigaki Okinawa Japan (2012-03-06)  
Latitude / Longitude  
Habitat (Isolation source) Brackish water (Plant (Mangrove))  
History < Nakayama, Takeshi < Kamikawa, Ryoma < Taira, Misaka  
Isolator (Date of isolation) Taira, Misaka (2012-03-**)  
Identified by Mayama, Shigeki  
State of strain Cryopreservation; Unialgal; Clonal; Axenic  
Culture condition
(Preculture condition)
Medium:  ESM + CB-V  
Temperature:  20 C
Light intensity:  0 µmol photons/m2/sec,
Duration:  21 D  
Gene information Mitochondrial DNA ( AP018509 ) , Plastid DNA ( AP018508 ) , 16S rRNA ( AB899716 ) , 28S rRNA ( AB899702 )  
Cell size (min - max) - 20 μm  
Organization Unicellular 
Characteristics Heterotrophic ; Benthic ; Nonphotosynthetic plastids ; Genome decoded strain (Kamikawa et al. 2018)  
Other strain no. Other strain no. : A4  
Remarks Cryopreserved; Axenic 
Movie  
Reference
Kamikawa, R., Azuma, T., Ishii, K-I., Matsuno, Y., Miyashita, H. 2018 Diversity of organellar genomes in non-photosynthetic diatoms. Protist, 169, 351-361.
Keywords: ATP synthase complex; mitochondria; Nitzschia; plastids; vitamin B1
Strain(s): 272935763581 
PubMed: 29803116
DOI: 10.1016/j.protis.2018.04.009

Kamikawa, R., Yubuki, N., Yoshida, M., Taira, M., Nakamura, N., Ishida, K-I., Leander, B. S., Miyashita, H., Hashimoto, T., Mayama, S., Inagaki, Y. 2015 Multiple losses of photosynthesis in Nitzschia (Bacillariophyceae). Phycol. Res., 63, 19-28.
Keywords: apochlorotic diatoms; genetic diversity; large subunit rRNA; molecular phylogenetic analysis; nonphotosynthetic plastids; plastid 16S rRNA
Strain(s): 357635773578357935803581 
DOI: 10.1111/pre.12072

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