The Microbial Culture Collection at the National Institute for Environmental Studies

Strain Data

Strain number NIES-3575  
Phylum Chlorophyta   Class Chlorophyceae 
Scientific name Koshicola spirodelophila Watanabe, Fučíková & Lewis  
Former name  
Common name Green alga  
Locality (Date of collection) Gofuku Toyama Toyama Japan (2005-12-01)  
Habitat (Isolation source) Freshwater (Plant)  
History < Watanabe, Shin  
Isolator (Date of isolation) Watanabe, Shin (2005-12-01)  
Identified by Watanabe, Shin  
State of strain Unialgal; Clonal; Non-axenic  
Preservation state
(State of preservation)
Culture condition
( Preculture condition )
Medium:  9:1 agar medium (S)  
Temperature: 20 C
Light intensity: 3-5 µmol photons/m2/sec     L/D cycle: 10L:14D
Duration: 3 M  
Gene information 18S rRNA ( AB936292 ) , 18S-ITS1-5.8S-ITS2-28S ( KT693223 )
Cell size (min - max) 12 - 55 μm   Organization  
Characteristics Attached ; Authentic strain (Watanabe, S. et al. 2016)  
Other strain no. Other strain no. : W1C4  

1788)   Watanabe, S., Fučíková, K., Lewis, L. A., Lewis P. O. 2016 Hiding in plain sight: Koshicola spirodelophila gen. et sp. nov. (Chaetopeltidales, Chlorophyceae), a novel green alga associated with the aquatic angiosperm Spirodela polyrhiza. Am. J. Bot., 103, 865-875. , Keywords: biodiversity; chloroplast genome; epiphyte; gene loss; pyrenoid; transmission electron microscopy , Strain(s): 3575