The Microbial Culture Collection at the National Institute for Environmental Studies

Strain Data

A big image opens when the thumbnail is clicked.

Strain number NIES-85  
Phylum Heterokontophyta   Class Raphidophyceae 
Scientific name Chattonella marina (Subrahmanyan) Y.Hara et Chihara var. antiqua (Hada) Demura et Kawachi  
Synonym -----  
Former name Chattonella antiqua (Hada) Ono  
Common name  
Locality (Date of collection) Shodo Isl. Shodoshima Kagawa Japan (1978-07-21)  
Habitat (Isolation source) Marine  
History < KAGAWA  
Isolator (Date of isolation) Yoshimatsu, Sadaaki (-----)  
Identified by Yoshimatsu, Sadaaki  
State of strain Unialgal; Clonal; Axenic[2013 Jan]  
Preservation state
(State of preservation)
Subculture  
Culture condition
( Preculture condition )
Medium:  f/2ESM  
Temperature: 20 C
Light intensity: 32-40 µmol photons/m2/sec     L/D cycle: 12L:12D
Duration: 1 M  
Gene information ITS1-5.8S-ITS2 ( AB334311 ) , CO1 ( AB334448 ) , rbcL ( AB334375 )
Cell size (min - max) - 43 μm   Organization Unicellular; Flagellate 
Characteristics Red tide  
Other strain no. Other strain no. : KGW-8-5  
Remarks Fragile species to transportation stresses 

Reference
79)   Demura, M., Noël, M.-H., Kasai, F., Watanabe, M. M., Kawachi, M. 2009 Taxonomic revision of Chattonella antiqua, C. marina and C. ovata (Raphidophyceae) based on their morphological characteristics and genetic diversity. Phycologia, 48, 518-535. , Keywords: Chattonella antiqua; Chattonella marina; Chattonella ovata; COI; COX1; Genetic diversity; ITS; Microsatellite; Morphology; Phylogeny; Population; Raphidophyceae; rbcL; Speciation; Taxonomy , Strain(s): 12356910148384858611311411511611812114514616129355755855956160367171684918721873 

226)   Hiroishi, S., Uchida, A., Nagasaki, K., Ishida, Y. 1988 A new method for identification of inter- and intra-species of the red tide algae Chattonella antiqua and Chattonella marina (Raphidophyceae) by means of monoclonal antibodies. J. Phycol., 24, 442-444. , Keywords: Chattonella; identification; immunofluorescence; inter-species; intra-species; method; monoclonal antibody; red tide , Strain(s): 121483848586113114115116118121 

227)   Hiroishi, S., Uchida, A., Nagasaki, K., Ishida, Y. 1989 A new method for inter- and intra-species identification of red tide algae Chattonella antiqua and Chattonella marina by means of monoclonal antibodies. In Red Tides: Biology, Environmental Science, and Toxicology, Eds. by Okaichi, T., Anderson, D. M. & Nemoto, T., Elsevier Science Pub., New York, p. 299-300. , Strain(s): 8586118121