The Microbial Culture Collection at the National Institute for Environmental Studies

Strain Data

Strain number NIES-3388  
Phylum Chlorophyta   Class Chlorophyceae 
Scientific name Colemanosphaera charkowiensis (Korshikov) Nozaki et al.  
Synonym Pandorina charkowiensis Korshikov  
Former name  
Common name Green alga  
Locality (Date of collection) Isanuma Pond Kawagoe Saitama Japan (2013-06-15)  
Habitat (Isolation source) Freshwater (Ditch water)  
History < Nozaki, Hisayoshi  
Isolator (Date of isolation) Nozaki, Hisayoshi (2013-06-15)  
Identified by Nozaki, Hisayoshi  
State of strain Unialgal; Clonal; Non-axenic  
Preservation state
(State of preservation)
Culture condition
( Preculture condition )
Medium:  AF-6VTAC  
Temperature: 20 C
Light intensity: 20 µmol photons/m2/sec     L/D cycle: 10L:14D
Duration: 1 M  
Gene information 18S rRNA ( LC086350 ) , 18S-ITS1-5.8S-ITS2-28S ( AB905585 )
Cell size (min - max)   Organization  
Characteristics Planktonic ; Dioecious; Anisogamous; Resting spore-forming; Authentic strain, Epitype(Nozaki, H. et al. 2014)  
Other strain no. Other strain no. : 2013-0615-IC-7  

957)   Nakada, T., Ito, T., Tomita, M. 2016 18S ribosomal RNA gene phylogeny of a colonial volvocalean lineage (Tetrabanaceae-Goniaceae-Volvocaceae, Volvocales, Chlorophyceae) and its close relatives. J. Jpn. Bot., 91, 345-354. , Keywords: 18S rRNA; colonial; green algae; molecular phylogeny; unicellular; Volvocales , Strain(s): 418456460474541544545564566571654666721725728735737867877879880881882883136222422246226625673388 

1133)   Nozaki, H., Yamada, K. T., Takahashi, F., Matsuzaki, R., Nakada, T. 2014 New "missing link" genus of the colonial volvocine green algae gives insights into the evolution of oogamy. BMC Evol. Biol., 14, 37 (article ID). , Strain(s): 3382338333843385338633873388