The Microbial Culture Collection at the National Institute for Environmental Studies

Strain Data

Strain number NIES-3383  
Phylum Chlorophyta   Class Chlorophyceae 
Scientific name Colemanosphaera charkowiensis (Korshikov) Nozaki et al.  
Synonym Pandorina charkowiensis Korshikov  
Former name  
Common name Green alga  
Locality (Date of collection) Isanuma Pond Kawagoe Saitama Japan (2005-06-24)  
Habitat (Isolation source) Freshwater (Pond water)  
History < Yamada, Toshihiro  
Isolator (Date of isolation) Yamada, Toshihiro (2005-06-24)  
Identified by Nozaki, Hisayoshi  
State of strain Unialgal; Clonal; Non-axenic  
Preservation state
(State of preservation)
Culture condition
( Preculture condition )
Medium:  AF-6VTAC  
Temperature: 20 C
Light intensity: 20 µmol photons/m2/sec     L/D cycle: 10L:14D
Duration: 1 M  
Gene information 18S-ITS1-5.8S-ITS2-28S ( AB905583 ) , rbcL ( AB905591 ) , psbC ( AB905597 ) , psaB ( AB905595 ) , psaA ( AB905593 ) , atpB ( AB905589 )
Cell size (min - max)   Organization  
Characteristics Planktonic  
Other strain no. Other strain no. : Isa 7-1  

1133)   Nozaki, H., Yamada, K. T., Takahashi, F., Matsuzaki, R., Nakada, T. 2014 New "missing link" genus of the colonial volvocine green algae gives insights into the evolution of oogamy. BMC Evol. Biol., 14, 37 (article ID). , Strain(s): 3382338333843385338633873388