The Microbial Culture Collection at the National Institute for Environmental Studies

Strain Data

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Strain number NIES-512  
Phylum Cyanophyta   Class Cyanophyceae 
Scientific name Pseudanabaena foetida Niiyama, Tuji & Ichise var. intermedia Tuji & Niiyama  
Synonym Phormidium tenue Gomont; Pseudanabaena galeata Böcher  
Former name Formerly identified as Pseudanabaena galeata, re-identified by Tuji & Niiyama 2016[2016 Oct]; Phormidium tenue  
Common name Blue-green alga; Cyanobacteria  
Locality (Date of collection) Nagoya Castle Nagoya Aichi Japan (1981-11-01)  
Habitat (Isolation source) Freshwater (Pond water)  
History < Yamada, Naoki  
Isolator (Date of isolation) Yamada, Naoki (1985-05-22)  
Identified by Tuji, Akihiro; Niiyama, Yuko  
State of strain Unialgal; Non-clonal; Axenic[2014 Mar]  
Preservation state
(State of preservation)
Cryopreservation  
Culture condition
( Preculture condition )
Medium:  CT  
Temperature: 20(20) C
Light intensity: 4-10(13-21) µmol photons/m2/sec     L/D cycle: 10L:14D
Duration: 20 D(7 D)  
Gene information 16S rRNA ( AB042838 ) , pgmtc ( AB826230 ) , pgmtf ( AB826229 ) , 16S rRNA ( LC153790 )
Cell size (min - max) 2 - 8 μm   Organization Filamentous 
Characteristics Offensive odor , 2-MIB production (Iwase, S. & Abe, T. et al. 2010) , Type strain  
Other strain no. Other strain no. : PM-81A  
Remarks Unstable; Cryopreserved 

Reference
90)   Chiu, Y.-T., Yen, H.-K., Lin, T.-F. 2016 An alternativemethodtoquantify2-MIB producing cyanobacteriain in drinking water reservoirs: Method development and field applications. Environ. Res., 151, 618-627. , Keywords: Cyanobacteria; 2-methylisoborneol(2-MIB); 2-MIB-synthesis gene(mibC gene); Tasteandodor; Real-timePCR , Strain(s): 8151282490521042116 

334)   Hodoki, Y., Ohbayashi, K., Kobayashi, Y., Okuda, N., Nakano, S. 2012 Detection and identification of potentially toxic cyanobacteria: ubiquitous distribution of Microcystis aeruginosa and Cuspidothrix issatschenkoi in Japanese lakes. Harmful Algae, 16, 49-57. , Keywords: Anatoxin-a; Automated rRNA intergenic spacer analysis (ARISA); Cuspidothrix issatschenkoi; Cyanotoxin; Microcystin; Microcystis aeruginosa , Strain(s): 304077102111230512843905928932937991164516691698172919502116 

364)   Hosoya, R., Hamana, K., Isobe, M., Yokota, A. 2005 Polyamine distribution profiles within Cyanobacteria. Microbiol. Cult. Coll., 21, 3-8. , Keywords: cyanobacteria; homospermidine; polyamine; spermidine , Strain(s): 41507378798081512515592593610846847 

435)   Ishibashi, Y., Oikawa, E., Honda, H., Adachi, T., Nakatochi, M. 2007 Analyses of growth factors of taste and odor producing algae by a fuzzy neural network. Environ. Eng. Res. [Kankyô-kôgaku Kenkyû Ronbunshû], 44, 255-263 (in Japanese with English summary). , Keywords: musty odor; Phormidium tenue; Uroglena americana; fuzzy neural network; wind speed , Strain(s): 512 

493)   Iwase, S., Abe, T. 2010 Identification and change in concentration of musty-odor compounds during frowth in blue-green algae. J. Sch. Marine Sci. Tech., Tokai Univ., 8, 27-33. , Strain(s): 5122119 

520)   Kakimoto, M., Ishikawa, T., Miyagi, A., Saito, K., Miyazaki, M., Asaeda, T., Yamaguchi, M., Uchiyama, H., Kawai-Yamada, M. 2014 Culture temperature affects gene expression and metabolic pathways in the 2-methylisoborneol-producing cyanobacterium Pseudanabaena galeata. J. Plant Res., 171, 292-300. , Keywords: 2-MIB; Cyanobacteria; Gene expression; Metabolomics; Temperature-induction , Strain(s): 512 

607)   Kaya, K., Shiraishi, F., Uchida, H., Sano, T. 2011 A novel retinoic acid analogue, 7-hydroxy retinoic acid, isolated from cyanobacteria. Biochim. Biophys. Acta, 1810, 414-419. , Keywords: 7-hydroxy retinoic acid; RAR agonistic activity; cyanobacteria; UV stability , Strain(s): 444546512610931 

743)   Lee, T., Tsuzuki, M., Takeuchi, T., Yokoyama, K., Karube, I. 1994 In vivo fluorometric method for early detection of cyanobacterial waterblooms. J. Appl. Phycol., 6, 489-495. , Keywords: cyanobacteria; waterblooms; in vivo fluorescence; monitoring; Microcystis aeruginosa , Strain(s): 298512 

744)   Lee, T., Tsuzuki, M., Takeuchi, T., Yokoyama, K., Karube, I. 1995 Quantitative determination of cyanobacteria in mixed phytoplankton assemblages by an in vivo fluorimetric method. Anal. Chim. Acta, 302, 81-87. , Keywords: cyanobacteria; quantitative estimation; phycocyanin; waterblooms; Microcystis aeruginosa , Strain(s): 298512 

904)   Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. , Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection , Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

944)   Nagai, T., Taya, K., Annoh, H., Ishihara, S. 2013 Application of a fluorometric microplate algal toxicity assay for riverine periphytic algal species. Ecotox. Environ. Safe., 94, 37-44. , Keywords: Attached algae; In-vivo fluorescence; Pesticides; Species sensitivity distribution , Strain(s): 357173414487512797 

945)   Nagai, T., Taya, K., Yoda, I. 2016 Comparative toxicity of 20 herbicides to 5 periphytic algae and the relationship with mode of action. Environ. Toxicol. Chem., 35, 368-375. , Keywords: Attached algae; In-vivo fluorescence; Pesticides; Species sensitivity distribution , Strain(s): 71487512797 

979)   Nakai, S., Inoue, Y., Hosomi, M., Murakami, A. 1999 Growth inhibition of blue-green algae by allelopathic effects of macrophytes. Wat. Sci. Tech., 39, 47-53. , Keywords: allelopathy; blue-green algae; coexistence; growth inhibition; macrophyte; Myriophyllum spicatum , Strain(s): 7587512 

983)   Nakai, S., Yamane, S., Hosomi, M. 2000 Algal growth inhibition effects of Myriophyllum spicatum-releasing four allelopathic polyphenols. J. Jpn. Soc. Wat. Environ., 23, 726-730 (in Japanese with English summary). , Keywords: allelochemical; polyphenol; algae; concentration-dependent synergistic growth inhibition; autoxidation , Strain(s): 35718796489512 

1168)   Ohko, Y., Nagao, Y., Okano, K., Sugiura, N., Fukuda, A., Yang, Y., Negishi, N., Takeuchi, M., Hanada, S. 2009 Prevention of Phormidium tenue biofilm formation by TiO2 photocatalysis. Microbes. Environ., 24, 241-245. , Keywords: Phormidium tenue; cyanobacteria; TiO2 photocatalyst; anti-biofilm; UV light , Strain(s): 512 

1201)   Oikawa, E. Ishibashi, Y., 2004 Species specificity of musty odor producing Phormidium tenue in Lake Kamafusa. Wat. Sci. Tech., 49, 41-46. , Keywords: 16S-rDNA sequence; 2-methylisoborneol; musty odor; Phormidium tenue phylogenetic tree , Strain(s): 213033417376512611 

1202)   Oikawa, E., Ishibashi, Y., Abe, T., Umetsu, H. 2000 Phylogenetic classification of musty odor and/or toxic compounds producing cyanobacteria. Environ. Eng. Res., 37, 183-191 (in Japanese with English summary). , Keywords: cyanobacteria; musty odor; 16S ribosomal RNA; PCR; phylogenetic trees , Strain(s): 213033417376512 

1203)   Oikawa, E., Ohnuma, T., Shimizu, A., Ishibashi, Y. 1994 Phylogenetic studies on musty odor producing cyanobacteria using the 16S ribosomal RNA [16S ribosômu RNA enki-hairetsu no hikaku ni yoru kabi-shû sansei ransô-rui no bunrui]. J. Jpn. Water Works Assoc. [Suidô-Kyôkai Zasshi], 63(12), 56-63 (in Japanese without English title). , Keywords: musty odor; gene; cyanobacteria , Strain(s): 3676512 

1690)   Tuji, A., Niiyama, Y. 2016 The identity and phylogeny of Pseudanabaena Strain, NIES-512, producing 2-methylisoborneol (2-MIB). Bull. Natl. Mus. Nat. Sci., Ser. B, 42, 83-89. , Keywords: 2-MIB; Pseudanabaena foetida var. intermedia; Pseudanabaena foetida var. subfoetida; secondary structure , Strain(s): 512 

1825)   Yamada, N., Aoyama, K., Yamada, M., Hamamura, N. 1985 Studies on earthy-musty odor in natural water (1). Growth characteristics and 2-methylisoborneol production of Phormidium tenue. Jpn. J. Water Poll. Res., 8, 515-521 (in Japanese with English summary). , Keywords: blue-green algae; Phormidium tenue; growth characteristics; headspace-GC analysis; 2-methylisoborneol , Strain(s): 512 

1826)   Yamada, N., Aoyama, K., Yamada, M., Hamamura, N. 1986 Studies on earthy-musty odor in natural water (3). Isolation of bacteria-free Phormidium tenue and the effect of associated bacteria on the growth of axenic P. tenue. Jpn. J. Water Poll. Res., 9, 379-385 (in Japanese with English summary). , Keywords: Phormidium tenue; axenic; growth; 2-methylisoborneol; bacteria interaction , Strain(s): 512 

1936)   Zhou, S., Nakai, S., Hosomi, M., Sezaki, Y., Tominaga, M. 2004 Inhibition of cyanobacterial growth by allelopathy of reed. Jpn. J. Water Treat. Biol., 40, 23-28 (in Japanese with English summary). , Keywords: reed; Phormidium tenue; cyanobacteria; inhibition; allelopathy , Strain(s): 512 

1957)   Oshimi, M., Fujimoto, N., Ohnishi, A., Suzuki, M., Akiba, M., Kunikane, S. 2005 Isolation of bacteria lysing musty odor producing alga Phormidium tenue and its lytic characteristics. J. Jpn. Soc. Wat. Environ., 28, 379-384 (in Japanese with English summary). , Keywords: alga-lytic bacteria; musty odor; Phormidium tenue; Pseudomonas KA6 , Strain(s): 96211227244298512817 

1963)   永井孝志 (Nagai, T. et al.) 2014 河川付着藻類を用いた農薬の毒性試験マニュアル Ver. 1.0 独立行政法人農業環境技術研究所 化学物質環境動態・影響評価リサーチプロジェクト 52 pp. , Strain(s): 71487512797