Strain number NIES-2838  
Phylum Heterokontophyta  
Class Bacillariophyceae  
Scientific name Skeletonema costatum (Grev.) Cleve emend. Zingone & Sarno  
Synonym Melosira costata Greville  
Former name  
Common name Centric diatom  
Locality (Date of collection) Dokai Bay Kitakyushu Fukuoka Japan (2007-10-02)  
Latitude / Longitude 33.88388888 / 130.78722222 
Habitat (Isolation source) Marine (Seawater)  
History < Yamada, Machiko  
Isolator (Date of isolation) Yamada, Machiko (2007-10-02)  
Identified by Yamada, Machiko  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  ESMMNK  
Temperature:  15 C
Light intensity:  3-10 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  21 D  
Gene information 28S rRNA ( AB572617 ) , 18S rRNA ( AB948138 ) , CO1 ( AB706217 )  
Cell size (min - max)  
Organization Unicellular 
Characteristics Red tide (Yamada et al. 2010) ; Planktonic  
Other strain no. Other strain no. : FDK009  
Remarks Unstable 
Movie  
Reference
Yamada, M., Otsubo, M., Tsutsumi, Y., Mizota, C., Nakamura, Y., Takahashi, K., Iwataki M. 2017 Utility of mitochondrial-encoded cytochrome c oxidase I gene for phylogenetic analysis and species identification of the planktonic diatom genus Skeletonema. Phycol. Res., 65, 217-225.
Keywords: cox1; genetic marker; LSU rDNA; phylogenetictree; species delimitation; SSU rDNA
Strain(s): 16223283728382842 
DOI: 10.1111/pre.12179

Kaeriyama, H., Katsuki, E., Otsubo, M., Yamada, K., Ichimi, K., Tada, K., Harrison, P. J. 2011 Effects of temperature and irradiance on growth of strains belonging to seven Skeletonema species isolated from Dokai Bay, southern Japan. Eur. J. Phycol., 46, 113-124.
Keywords: Dokai Bay; irradiance; physiology; Skeletonema species; specific growth rate; temperature
Strain(s): 2837283828392841284228432844 
DOI: 10.1080/09670262.2011.565128

Yamada, M., Katsuki, E., Otsubo, M., Kawaguchi, M., Ichimi, K., Keriyama, H., Tada, K., Harrison, P. J. 2010 Species diversity of the genus Skeltonema (Bacillariophyceae) in the Industrial Harbor Dokai Bay, Japan. J. Oceanogr., 66, 755-771.
Keywords: Skeletonema; species diversity; isolation characteristics; Dokai Bay; LSU rDNA; SSU rDNA; SEM
Strain(s): 2837283828392841284228432844 
DOI: 10.1007/s10872-010-0062-4

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