Strain number NIES-2744  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Microglena opisthopyren (Skuja) Demchenko, Mikhailyuk & Pröschold  
Synonym Chlamydomonas opisthopyren Skuja; Chlamydomonas macroplastida J.W.G. Lund f. macrostigma Bourrelly; Chlamydomonas pseudomacrostigma Péterfi  
Former name Chlamydomonas pseudomacrostigma L.Ş. Péterfi ex H.Ettl  
Common name Green alga  
Locality (Date of collection) Tsuruoka Yamagata Japan (2008-05-29)  
Latitude / Longitude  
Habitat (Isolation source) Freshwater (Sediment)  
History < Nakada, Takashi  
Isolator (Date of isolation) Nakada, Takashi (2008-07-23)  
Identified by Nakada, Takashi  
State of strain Subculture; Unialgal; Clonal; Axenic[2013 Dec]  
Culture condition
(Preculture condition)
Medium:  AF-6  
Temperature:  20 C
Light intensity:  12-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M  
Gene information 18S rRNA ( AB694004 ) , 5.8S-ITS2-28S ( AB872921 )  
Cell size (min - max)  
Organization Unicellular; Flagellate 
Characteristics Phototaxis ; Authentic strain, Epitype (Nakada, T. et al. 2014)  
Other strain no. Other strain no. : TkS0811B3  
Nakada, T., Tomita, M. 2014 Light microscopy and phylogenetic asalyses of Chlamydomonas species (Volvocales, Chlorophyceae). II. Molecular phylogeny, secondary Microglena opisthopyren, and M. media, comb. nov. Acta Phytotax. Geobot., 65, 67-73.
Keywords: Chlamydomonas; ITS-2; Microglena; molecular phylogeny; phycology; synonymy
Strain(s): 27432744 

Nakada, T., Nozaki, H., Tomita, M. 2012 Light microscopy and phylogenetic analyses of Chlamydomonas species (Volvocales, Chlorophyceae). I. Chlamydomonas pseudomacrostigma and Chlamydomonas media. Acta Phytotax. Geobot., 62, 69-78.
Keywords: 18S rRNA; Chlamydomonas media; Chlamydomonas pseudomacrostigma; light microscopy; molecular phylogeny, phycology
Strain(s): 27432744 

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