Strain Data

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Strain number		NIES-12
Phylum		Dinophyta
Class		Dinophyceae
Scientific name		Prorocentrum micans Ehrenberg
Synonym
Former name
Common name		Dinoflagellate
Locality (Date of collection)		Osaka Bay Osaka Japan (1981-07-**)
Latitude / Longitude
Habitat (Isolation source)		Marine
History		< Yamochi, Susumu
Isolator (Date of isolation)		Yamochi, Susumu
Identified by
State of strain		Subculture; Unialgal; Clonal; Axenic[2018 Feb]
Culture condition (Preculture condition)		Medium: ESM Temperature: 20 C Light intensity: 32-40 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 1 M
Gene information		CO1 ( AB000134 AB000133 ) , psbA ( AB025585 )
Cell size (min - max)		30 - 38 μm
Organization		Unicellular; Flagellate
Characteristics		Red tide
Other strain no.		Other strain no. : OPm
Remarks		Fragile species to transportation stresses; Read and agree "How to order 4.1"; Axenic
Movie

Reference
Hosoi-Tanabe, S., Sako, Y. 2005 Species-speciﬁc detection and quantiﬁcation of toxic marine Dinoﬂagellates Alexandrium tamarense and A. catenella by reai-time PCR assay. Mar. Biotechnol., 7, 506-514. Keywords: toxic dinoﬂagellate; — Alexandrium; paralytic shellfish poisoning (PSP); real-time PCR; species-specific identification; quantification Strain(s): 1, 7, 12, 293, 331, 559, 603, 670 PubMed: 16007374 DOI: 10.1007/s10126-004-4128-4 Hamana, K., Sakamoto, A., Nishina, M., Niitsu, M. 2004 Cellular polyamine profile of the phyla Dinophyta, Apicomplexa, Ciliophora, Euglenozoa, Cercozoa and Heterokonta. J. Gen. Appl. Microbiol., 50, 297-303. Keywords: Apicomlexa; Cercozoa; Ciliophora; Dinophyta; Euglenozoa; Heterokonta; norspermidine; polyamine Strain(s): 12, 286, 304, 331, 381, 387, 463 PubMed: 15747233 DOI: 10.2323/jgam.50.297 Sako, Y., Hosoi-Tanabe, S., Uchida, A. 2004 Fluorescence in situ hybridization using rRNA-targeted probes for simple and rapid identification of the toxic dinoflagelates Alexandrium tamarense and Alexandrium catenella. J. Phycol., 40, 598-605. Keywords: Alexandrium; FISH method; LSU rRNA; molecular identiﬁcation; paralytic shellﬁsh poisoning (PSP); probe; toxic dinoﬂagellate Strain(s): 1, 7, 12, 331 DOI: 10.1111/j.1529-8817.2004.03035.x Takishita, K., Uchida, A. 1999 Molecular cloning and nucleotide sequence analysis of psbA from the dinoflagellates: origin of the dinoflagellate plastid. Phycol. Res., 47, 207-216. Keywords: D1; dinoflagellate; phylogenetic analysis; plastid; psbA Strain(s): 12, 331 DOI: 10.1046/j.1440-1835.1999.00163.x Inagaki, Y., Ehara, M., Watanabe, K. I., Hayashi-Ishimaru, Y., Ohama, T. 1998 Directionally evolving genetic code: the UGA codon from stop to tryptophan in mitochondria. J. Mol. Evol., 47, 378-384. Keywords: COXI phylogenetic tree; UGA codon; deviant genetic code; directional codon reassignment Strain(s): 12, 227, 274 PubMed: 9767683 DOI: 10.1007/PL00006395 Inagaki, Y., Hayashi-Ishimaru, Y., Ehara, M., Igarashi, I., Ohama, T. 1997 Algae or protozoa: phylogenetic position of euglenophytes and dinoflagellates as inferred from mitochondrial sequences. J. Mol. Evol., 45, 295-300. Keywords: mitochondrial COXI gene; Dinoflagellata; Apicomplexa; Kinetoplastida; Euglenophyta; endosymbiosis; genetic code; malarian circle Strain(s): 12, 369, 381 DOI: 10.1007/PL00006233 Yasumoto, T. 1992 Toxin of the marine micro-algae [Kaiyô bisaisôrui no Tokishin]. BIO Medica, 7, 57-62 (in Japanese without English title). Strain(s): 1, 6, 12, 15, 369 Nagai, H., Satake, M., Murata, M., Yasumoto, T. 1990 Screening of marine phytoplankton for antifungal substances. In Toxic Marine Phyto-plankton, Eds. by Graneli, E., Sundstrom, B., Edler, L. & Anderson, D. M., Elsevier Science Pub., New York, p. 385-390. Strain(s): 1, 6, 12, 15, 369 Uye, S., Takamatsu, K. 1990 Feeding inter-actions between planktonic copepods and red-tide flagellates from Japanese coastal waters. Mar. Ecol. Prog. Ser., 59, 97-107. Strain(s): 12, 15, 145, 221, 235, 251

Reference

Hosoi-Tanabe, S., Sako, Y. 2005 Species-speciﬁc detection and quantiﬁcation of toxic marine Dinoﬂagellates Alexandrium tamarense and A. catenella by reai-time PCR assay. Mar. Biotechnol., 7, 506-514.
Keywords: toxic dinoﬂagellate; — Alexandrium; paralytic shellfish poisoning (PSP); real-time PCR; species-specific identification; quantification
Strain(s): 1, 7, 12, 293, 331, 559, 603, 670
PubMed: 16007374
DOI: 10.1007/s10126-004-4128-4

Hamana, K., Sakamoto, A., Nishina, M., Niitsu, M. 2004 Cellular polyamine profile of the phyla Dinophyta, Apicomplexa, Ciliophora, Euglenozoa, Cercozoa and Heterokonta. J. Gen. Appl. Microbiol., 50, 297-303.
Keywords: Apicomlexa; Cercozoa; Ciliophora; Dinophyta; Euglenozoa; Heterokonta; norspermidine; polyamine
Strain(s): 12, 286, 304, 331, 381, 387, 463
PubMed: 15747233
DOI: 10.2323/jgam.50.297

Sako, Y., Hosoi-Tanabe, S., Uchida, A. 2004 Fluorescence in situ hybridization using rRNA-targeted probes for simple and rapid identification of the toxic dinoflagelates Alexandrium tamarense and Alexandrium catenella. J. Phycol., 40, 598-605.
Keywords: Alexandrium; FISH method; LSU rRNA; molecular identiﬁcation; paralytic shellﬁsh poisoning (PSP); probe; toxic dinoﬂagellate
Strain(s): 1, 7, 12, 331
DOI: 10.1111/j.1529-8817.2004.03035.x

Takishita, K., Uchida, A. 1999 Molecular cloning and nucleotide sequence analysis of psbA from the dinoflagellates: origin of the dinoflagellate plastid. Phycol. Res., 47, 207-216.
Keywords: D1; dinoflagellate; phylogenetic analysis; plastid; psbA
Strain(s): 12, 331
DOI: 10.1046/j.1440-1835.1999.00163.x

Inagaki, Y., Ehara, M., Watanabe, K. I., Hayashi-Ishimaru, Y., Ohama, T. 1998 Directionally evolving genetic code: the UGA codon from stop to tryptophan in mitochondria. J. Mol. Evol., 47, 378-384.
Keywords: COXI phylogenetic tree; UGA codon; deviant genetic code; directional codon reassignment
Strain(s): 12, 227, 274
PubMed: 9767683
DOI: 10.1007/PL00006395

Inagaki, Y., Hayashi-Ishimaru, Y., Ehara, M., Igarashi, I., Ohama, T. 1997 Algae or protozoa: phylogenetic position of euglenophytes and dinoflagellates as inferred from mitochondrial sequences. J. Mol. Evol., 45, 295-300.
Keywords: mitochondrial COXI gene; Dinoflagellata; Apicomplexa; Kinetoplastida; Euglenophyta; endosymbiosis; genetic code; malarian circle
Strain(s): 12, 369, 381
DOI: 10.1007/PL00006233

Yasumoto, T. 1992 Toxin of the marine micro-algae [Kaiyô bisaisôrui no Tokishin]. BIO Medica, 7, 57-62 (in Japanese without English title).
Strain(s): 1, 6, 12, 15, 369

Nagai, H., Satake, M., Murata, M., Yasumoto, T. 1990 Screening of marine phytoplankton for antifungal substances. In Toxic Marine Phyto-plankton, Eds. by Graneli, E., Sundstrom, B., Edler, L. & Anderson, D. M., Elsevier Science Pub., New York, p. 385-390.
Strain(s): 1, 6, 12, 15, 369

Uye, S., Takamatsu, K. 1990 Feeding inter-actions between planktonic copepods and red-tide flagellates from Japanese coastal waters. Mar. Ecol. Prog. Ser., 59, 97-107.
Strain(s): 12, 15, 145, 221, 235, 251

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